CAJ | 학술논문

背景：透骨消痛胶囊是治疗骨性关节炎的临床验方，作用机制尚未完全阐明。尿激酶型纤溶酶原激活系统参与软骨细胞外基质降解和滑膜增生，在骨性关节炎发生、发展中起重要作用。 　　目的：观察透骨消痛胶囊含药血清对体外培养的膝骨性关节炎大鼠滑膜细胞表达尿激酶型纤溶酶原激活剂、尿激酶型纤溶酶原激活剂受体、纤溶酶原激活剂抑制剂、基质金属蛋白酶3、白细胞介素1β和肿瘤坏死因子α的影响，探讨透骨消痛胶囊防治膝骨性关节炎的作用机制。 　　方法：采用4%木瓜蛋白酶注射关节腔复制大鼠膝骨性关节炎模型；胶原酶消化法原代培养正常滑膜细胞与骨性关节炎滑膜细胞，将所培养滑膜细胞分为正常组、模型组和透骨消痛胶囊组，前2组细胞用空白血清培养，透骨消炎胶囊用含药血清培养；透骨消痛胶囊含药血清作用骨性关节炎滑膜细胞72 h后，采用Western Blot法检测滑膜细胞中尿激酶型纤溶酶原激活剂、尿激酶型纤溶酶原激活剂受体、纤溶酶原激活剂抑制剂、基质金属蛋白酶3、白细胞介素1β和肿瘤坏死因子α蛋白的表达。 　　结果与结论：透骨消痛胶囊含药血清组尿激酶型纤溶酶原激活剂、尿激酶型纤溶酶原激活剂受体、基质金属蛋白酶3、白细胞介素1β和肿瘤坏死因子α蛋白表达量均降低，纤溶酶原激活剂抑制剂表达量升高，与模型组相比均有显著性差异，提示透骨消痛胶囊治疗骨性关节炎的部分作用机制是有效抑制骨性关节炎滑膜细胞尿激酶型纤溶酶原激活剂、尿激酶型纤溶酶原激活剂受体、基质金属蛋白酶3、白细胞介素1β和肿瘤坏死因子α的表达，提高纤溶酶原激活剂抑制剂的表达。
배경：투골소통효낭시치료골성관절염적림상험방，작용궤제상미완전천명。뇨격매형섬용매원격활계통삼여연골세포외기질강해화활막증생，재골성관절염발생、발전중기중요작용。 　　목적：관찰투골소통효낭함약혈청대체외배양적슬골성관절염대서활막세포표체뇨격매형섬용매원격활제、뇨격매형섬용매원격활제수체、섬용매원격활제억제제、기질금속단백매3、백세포개소1β화종류배사인자α적영향，탐토투골소통효낭방치슬골성관절염적작용궤제。 　　방법：채용4%목과단백매주사관절강복제대서슬골성관절염모형；효원매소화법원대배양정상활막세포여골성관절염활막세포，장소배양활막세포분위정상조、모형조화투골소통효낭조，전2조세포용공백혈청배양，투골소염효낭용함약혈청배양；투골소통효낭함약혈청작용골성관절염활막세포72 h후，채용Western Blot법검측활막세포중뇨격매형섬용매원격활제、뇨격매형섬용매원격활제수체、섬용매원격활제억제제、기질금속단백매3、백세포개소1β화종류배사인자α단백적표체。 　　결과여결론：투골소통효낭함약혈청조뇨격매형섬용매원격활제、뇨격매형섬용매원격활제수체、기질금속단백매3、백세포개소1β화종류배사인자α단백표체량균강저，섬용매원격활제억제제표체량승고，여모형조상비균유현저성차이，제시투골소통효낭치료골성관절염적부분작용궤제시유효억제골성관절염활막세포뇨격매형섬용매원격활제、뇨격매형섬용매원격활제수체、기질금속단백매3、백세포개소1β화종류배사인자α적표체，제고섬용매원격활제억제제적표체。
BACKGROUND:Tougu Xiaotong Capsule (TGXTC) is a clinical prescription for the treatment of osteoarthritis;however, its mechanism has not been ful y elucidated. Urokinase-type plasminogen activator (uPA) system participating in the degradation of the extracel ular matrix of articular cartilage and hyperplasia of joint synovium plays an important role in the pathological process of osteoarthritis. OBJECTIVE:To investigate the effects of TGXTC medicated serum on the expression of uPA, uPA receptor (uPAR), plasminogen activator inhibitors (PAIs), matrix metal oproteinase-3 (MMP-3), interleukin-1 beta (IL-1β) and tumor necrosis factor-α(TNF-α) in osteoarthritis synovial cel s of rats and to discuss the mechanism by TGXTC medicated serum prevents and cures osteoarthritis. METHODS:Rat models with knee osteoarthritis were established by injecting 4%papain into the knee joint cavity. Primary synoviocytes and osteoarthritis synoviocytes were cultured with col agenase digestion method. The cultured synoviocytes were divided into normal group, model group and TGXTC group. The western blot method was adopted to detect uPA, uPAR, PAI, MMP-3, IL-1βand TNF-αprotein expression of synoviocytes after acting by TGXTC medicated serum for 72 hours. RESULTS AND CONCLUSION:The expression of uPA, uPAR, MMP-3, IL-1βand TNF-αwere decreased, while PAI was increased in the TGXTC group, and there were significant differences when compared with model group. In a word, TGXTC can significantly inhibit the expression of uPA, uPAR, MMP-3, IL-1β, TNF-α, and improve PAI expression in synoviocytes, which may partly explain the mechanism of the treatment of Tougu Xiaotong Capsule on osteoarthritis.