中国美容医学
中國美容醫學
중국미용의학
Chinese Journal of Aesthetic Medicine
2015年
18期
34-40
,共7页
余晶%马晓冬%郗敏%吴毅平
餘晶%馬曉鼕%郗敏%吳毅平
여정%마효동%치민%오의평
年龄%脂肪移植%存活率%组织重塑%超声造影
年齡%脂肪移植%存活率%組織重塑%超聲造影
년령%지방이식%존활솔%조직중소%초성조영
age%fat grafting%retention%tissue remodeling%contrast-enhanced ultrasound(CEUS)
目的:建立不同年龄大鼠来源的脂肪组织注射移植的动物模型,对比观察研究年龄因素对移植脂肪存活的影响,为临床脂肪移植应用提供依据.方法:取年轻(4周龄)、中年(10月龄)、老年(18月龄)三个年龄组的雌性SD大鼠的皮下脂肪组织,注射移植到同一只SD大鼠(10周龄)的背部皮下,在移植术后24h,48h, 72h,96h,5d,7d,14d,4周,8周,分别对脂肪移植物进行超声造影检查,然后取移植物标本,行形态学观察,并制作HE染色、Perilipin免疫荧光染色切片,行组织学观察,比较不同年龄来源的脂肪组织移植后的早期脂肪组织存活情况及远期存活率.结果:SD大鼠的颗粒脂肪组织在移植后出现从缺血退化到再生的动态组织重塑过程,早期仅有移植物最外层的脂肪细胞存活,随后出现血运重建、脂肪细胞再生等组织重塑过程,最终形成正常脂肪组织样结构.4周龄组的脂肪组织在移植后3d内存活脂肪细胞数目,移植后1周时脂肪存活区面积比,血流灌注恢复时间,新生的脂肪细胞数量,移植后8周时移植物存活指数明显优于10月龄、18月龄组(P<0.05);而10月龄与18月龄组间无明显差异(P>0.05).结论:年轻大鼠的脂肪组织在移植后,脂肪组织的早期存活、血运重建、脂肪细胞再生、远期脂肪组织存活率明显优于中、老年组;而中、老年组的脂肪组织在移植后远期存活率较低,且两者间无明显差异.
目的:建立不同年齡大鼠來源的脂肪組織註射移植的動物模型,對比觀察研究年齡因素對移植脂肪存活的影響,為臨床脂肪移植應用提供依據.方法:取年輕(4週齡)、中年(10月齡)、老年(18月齡)三箇年齡組的雌性SD大鼠的皮下脂肪組織,註射移植到同一隻SD大鼠(10週齡)的揹部皮下,在移植術後24h,48h, 72h,96h,5d,7d,14d,4週,8週,分彆對脂肪移植物進行超聲造影檢查,然後取移植物標本,行形態學觀察,併製作HE染色、Perilipin免疫熒光染色切片,行組織學觀察,比較不同年齡來源的脂肪組織移植後的早期脂肪組織存活情況及遠期存活率.結果:SD大鼠的顆粒脂肪組織在移植後齣現從缺血退化到再生的動態組織重塑過程,早期僅有移植物最外層的脂肪細胞存活,隨後齣現血運重建、脂肪細胞再生等組織重塑過程,最終形成正常脂肪組織樣結構.4週齡組的脂肪組織在移植後3d內存活脂肪細胞數目,移植後1週時脂肪存活區麵積比,血流灌註恢複時間,新生的脂肪細胞數量,移植後8週時移植物存活指數明顯優于10月齡、18月齡組(P<0.05);而10月齡與18月齡組間無明顯差異(P>0.05).結論:年輕大鼠的脂肪組織在移植後,脂肪組織的早期存活、血運重建、脂肪細胞再生、遠期脂肪組織存活率明顯優于中、老年組;而中、老年組的脂肪組織在移植後遠期存活率較低,且兩者間無明顯差異.
목적:건립불동년령대서래원적지방조직주사이식적동물모형,대비관찰연구년령인소대이식지방존활적영향,위림상지방이식응용제공의거.방법:취년경(4주령)、중년(10월령)、노년(18월령)삼개년령조적자성SD대서적피하지방조직,주사이식도동일지SD대서(10주령)적배부피하,재이식술후24h,48h, 72h,96h,5d,7d,14d,4주,8주,분별대지방이식물진행초성조영검사,연후취이식물표본,행형태학관찰,병제작HE염색、Perilipin면역형광염색절편,행조직학관찰,비교불동년령래원적지방조직이식후적조기지방조직존활정황급원기존활솔.결과:SD대서적과립지방조직재이식후출현종결혈퇴화도재생적동태조직중소과정,조기부유이식물최외층적지방세포존활,수후출현혈운중건、지방세포재생등조직중소과정,최종형성정상지방조직양결구.4주령조적지방조직재이식후3d내존활지방세포수목,이식후1주시지방존활구면적비,혈류관주회복시간,신생적지방세포수량,이식후8주시이식물존활지수명현우우10월령、18월령조(P<0.05);이10월령여18월령조간무명현차이(P>0.05).결론:년경대서적지방조직재이식후,지방조직적조기존활、혈운중건、지방세포재생、원기지방조직존활솔명현우우중、노년조;이중、노년조적지방조직재이식후원기존활솔교저,차량자간무명현차이.
Objective To expore the effect of donor age on the retention of fat grafting in rat modle,soas to supply evidence in clinical cases. Methods Fat grafts were obtained from young, middle-aged and aged(4 weeks,10 months, and 18 months old)female SD rats,and then injected into the back of a 10-week-old female SD rat.Ultrasonography and Contrast-enhanced ultrasound exam were taken on each fat grafts at 24h,48h,72h,96h,5d,1st week,2nd week,4th week and 8th week after operation,to record tissuse reperfusion dynamically,then samples were harvested and analyzed histologically for adipocyte viability,adipocyte regeneration,and fat grafting retention in different groups. Results Fat grafts from all ages underwent dynamic tissue remodeling after grafting,ischemic degeneration at early stage,then regeneration,approaching to normal adipose tissue finally. Fat grafts from 4-week-old SD rats showed signifiantly more vital adipocytes in 72h,larger survival zones at 1st week,and larger engraftment scores at 8th week,more new adipoctyes at 4th week,and more rapid reperfusion rate, respectively,than 10-month-old or 18-month-old SD rats(P<0.05).The discrepancy between 10-month-old and 18-month-old SD rats was not significant(P>0.05). Conclusion Fat grafts from young rats showed better adipocyte survival, regeneration,and final retention,than middle-aged and aged rats.Final fat grafting retention were similarly low in middle-aged and aged rats.
