CAJ | 학술논문

目的：建立离子色谱法测定食品中甜蜜素含量的分析方法。方法：不同基质的样品经相应方法提取和净化后，以 Dionex IonPacTMAS19（4 mm×250 mm）为分析柱，Dionex IonPacTMAG19（4 mm×50 mm）为保护柱，自动在线淋洗液发生器产生的高纯氢氧化钾溶液为淋洗液进行梯度淋洗，电导检测器检测，外标法定量。结果：该方法在0．50～10．0μg／mL 浓度有良好的线性关系，相关系数 r2为0．9997；不同种类食品中甜蜜素的加标回收率在92．7％～105．6％，相对标准偏差在0．08％～1．68％，方法检出限为0．15 mg／kg ，方法定量限为0．5 mg／kg。结论：该方法具有简便、快速、准确、灵敏度高、实用性强等优点，可用于固体、液体等不同形态不同基质食品中甜蜜素测定。
목적：건립리자색보법측정식품중첨밀소함량적분석방법。방법：불동기질적양품경상응방법제취화정화후，이 Dionex IonPacTMAS19（4 mm×250 mm）위분석주，Dionex IonPacTMAG19（4 mm×50 mm）위보호주，자동재선림세액발생기산생적고순경양화갑용액위림세액진행제도림세，전도검측기검측，외표법정량。결과：해방법재0．50～10．0μg／mL 농도유량호적선성관계，상관계수 r2위0．9997；불동충류식품중첨밀소적가표회수솔재92．7％～105．6％，상대표준편차재0．08％～1．68％，방법검출한위0．15 mg／kg ，방법정량한위0．5 mg／kg。결론：해방법구유간편、쾌속、준학、령민도고、실용성강등우점，가용우고체、액체등불동형태불동기질식품중첨밀소측정。
Obj ect:A method is developed for the determination of the content of sodium cyclamate in food by ion chromatography.Method:Samples of different matrix after corresponding extraction and purification methods,use Dionex IonPacTM AS19 (4 mm × 250 mm)as analytical column,Dionex IonPacTMAG19 (4 mm × 50 mm)as guard column,automatic on-line eluent generator to produce high-purity potassium hydroxide solution as eluent for gradient elution,conductance detector for detection and external standard method for quantitative analysis.Result:This method has a great linear relationship in the concentration range of 0.50~10.0μg/mL,the correlation coefficient r2 is 0.9997;the standard addition recoveries of sodium cyclamate in different kinds of foods are between 92.7%~105.6%,the relative standard deviations are in the range of 0.08%~1.68%,the method detection limit is 0.15 mg/kg,and the method quantitative limit is 0.5 mg/kg.Conclusion:This method is simple,rapid,accurate,with high sensitivity,strong practicability,etc.,it can be used to determine the content of sodium cyclamate in solid,liquid and other different morphological matrix of food.