CAJ | 학술논문

目的:研究补益营卫方、转化生长因子β1(TGFβ1)及碱性成纤维细胞生长因子(bFGF)对衰老人皮肤成纤维细胞c-Jun基因表达的影响.方法:通过连续传代培养复制衰老人皮肤成纤维细胞模型,通过中和抗体阻断TGFβ1及bFGF后,利用荧光定量PCR法检测衰老人皮肤成纤维细胞c-Jun基因表达.结果:人皮肤成纤维细胞衰老时,c-Jun基因表达水平24h和48h比人年轻皮肤成纤维细胞分别下降了70%和71%,72h则两者的表达水平相当.衰老人皮肤成纤维细胞补益营卫药物处理后显著提高了c-Jun基因的表达,相对于衰老人皮肤成纤维细胞,其24h、48h分别提高了4.67倍和2.12倍,而至72h其表达水平相当.中和TGF-β1蛋白后,衰老人皮肤成纤维细胞c-Jun基因的表达水平24h和72h分别是年轻人皮肤成纤维细胞的2.4倍和1.7倍.而48h时,年轻人皮肤成纤维细胞的基因表达水平是衰老人皮肤成纤维细胞的9.2倍.衰老人皮肤成纤维细胞补益营卫药物处理后显著提高了c-Jun基因的表达,48h和72h时c-Jun基因的表达水平分别是衰老人皮肤成纤维细胞的2.3倍和1.5倍.中和TGF-β1和bFGF蛋白后,c-Jun基因的表达水平随着时间的不同被明显下调;与年轻人皮肤成纤维细胞相比,24h、48h和72h衰老人皮肤成纤维细胞c-Jun基因的表达水平分别下降了64.5%、94.2%和73.7%.衰老人皮肤成纤维细胞补益营卫药物处理后c-Jun基因的表达随着时间的不同相对于衰老人皮肤成纤维细胞被显著上调.24h、48h和72h分别是衰老人皮肤成纤维细胞的9.7倍、3.6倍和9.8倍.纵向比较来看,TGF-β1蛋白对衰老人皮肤成纤维细胞c-Jun基因的表达存在复杂的调节作用.bFGF蛋白对衰老人皮肤成纤维细胞c-Jun基因表达有明显的促进作用.结论:衰老时人皮肤成纤维细胞c-Jun基因的表达紊乱.补益营卫方对衰老人皮肤成纤维细胞c-Jun基因表达具有促进作用;其促进c-Jun基因表达存在不依赖于TGF-β1和bFGF的途径.bFGF蛋白是TGF-β1信号调节途径的下游蛋白;是c-Jun基因表达的重要促进因子. 目的:研究補益營衛方、轉化生長因子β1(TGFβ1)及堿性成纖維細胞生長因子(bFGF)對衰老人皮膚成纖維細胞c-Jun基因錶達的影響.方法:通過連續傳代培養複製衰老人皮膚成纖維細胞模型,通過中和抗體阻斷TGFβ1及bFGF後,利用熒光定量PCR法檢測衰老人皮膚成纖維細胞c-Jun基因錶達.結果:人皮膚成纖維細胞衰老時,c-Jun基因錶達水平24h和48h比人年輕皮膚成纖維細胞分彆下降瞭70%和71%,72h則兩者的錶達水平相噹.衰老人皮膚成纖維細胞補益營衛藥物處理後顯著提高瞭c-Jun基因的錶達,相對于衰老人皮膚成纖維細胞,其24h、48h分彆提高瞭4.67倍和2.12倍,而至72h其錶達水平相噹.中和TGF-β1蛋白後,衰老人皮膚成纖維細胞c-Jun基因的錶達水平24h和72h分彆是年輕人皮膚成纖維細胞的2.4倍和1.7倍.而48h時,年輕人皮膚成纖維細胞的基因錶達水平是衰老人皮膚成纖維細胞的9.2倍.衰老人皮膚成纖維細胞補益營衛藥物處理後顯著提高瞭c-Jun基因的錶達,48h和72h時c-Jun基因的錶達水平分彆是衰老人皮膚成纖維細胞的2.3倍和1.5倍.中和TGF-β1和bFGF蛋白後,c-Jun基因的錶達水平隨著時間的不同被明顯下調;與年輕人皮膚成纖維細胞相比,24h、48h和72h衰老人皮膚成纖維細胞c-Jun基因的錶達水平分彆下降瞭64.5%、94.2%和73.7%.衰老人皮膚成纖維細胞補益營衛藥物處理後c-Jun基因的錶達隨著時間的不同相對于衰老人皮膚成纖維細胞被顯著上調.24h、48h和72h分彆是衰老人皮膚成纖維細胞的9.7倍、3.6倍和9.8倍.縱嚮比較來看,TGF-β1蛋白對衰老人皮膚成纖維細胞c-Jun基因的錶達存在複雜的調節作用.bFGF蛋白對衰老人皮膚成纖維細胞c-Jun基因錶達有明顯的促進作用.結論:衰老時人皮膚成纖維細胞c-Jun基因的錶達紊亂.補益營衛方對衰老人皮膚成纖維細胞c-Jun基因錶達具有促進作用;其促進c-Jun基因錶達存在不依賴于TGF-β1和bFGF的途徑.bFGF蛋白是TGF-β1信號調節途徑的下遊蛋白;是c-Jun基因錶達的重要促進因子.
목적:연구보익영위방、전화생장인자β1(TGFβ1)급감성성섬유세포생장인자(bFGF)대쇠노인피부성섬유세포c-Jun기인표체적영향.방법:통과련속전대배양복제쇠노인피부성섬유세포모형,통과중화항체조단TGFβ1급bFGF후,이용형광정량PCR법검측쇠노인피부성섬유세포c-Jun기인표체.결과:인피부성섬유세포쇠로시,c-Jun기인표체수평24h화48h비인년경피부성섬유세포분별하강료70%화71%,72h칙량자적표체수평상당.쇠노인피부성섬유세포보익영위약물처리후현저제고료c-Jun기인적표체,상대우쇠노인피부성섬유세포,기24h、48h분별제고료4.67배화2.12배,이지72h기표체수평상당.중화TGF-β1단백후,쇠노인피부성섬유세포c-Jun기인적표체수평24h화72h분별시년경인피부성섬유세포적2.4배화1.7배.이48h시,년경인피부성섬유세포적기인표체수평시쇠노인피부성섬유세포적9.2배.쇠노인피부성섬유세포보익영위약물처리후현저제고료c-Jun기인적표체,48h화72h시c-Jun기인적표체수평분별시쇠노인피부성섬유세포적2.3배화1.5배.중화TGF-β1화bFGF단백후,c-Jun기인적표체수평수착시간적불동피명현하조;여년경인피부성섬유세포상비,24h、48h화72h쇠노인피부성섬유세포c-Jun기인적표체수평분별하강료64.5%、94.2%화73.7%.쇠노인피부성섬유세포보익영위약물처리후c-Jun기인적표체수착시간적불동상대우쇠노인피부성섬유세포피현저상조.24h、48h화72h분별시쇠노인피부성섬유세포적9.7배、3.6배화9.8배.종향비교래간,TGF-β1단백대쇠노인피부성섬유세포c-Jun기인적표체존재복잡적조절작용.bFGF단백대쇠노인피부성섬유세포c-Jun기인표체유명현적촉진작용.결론:쇠로시인피부성섬유세포c-Jun기인적표체문란.보익영위방대쇠노인피부성섬유세포c-Jun기인표체구유촉진작용;기촉진c-Jun기인표체존재불의뢰우TGF-β1화bFGF적도경.bFGF단백시TGF-β1신호조절도경적하유단백;시c-Jun기인표체적중요촉진인자.
Objective To study the c-Jun gene expression influenced by the recipe of supplementing Ying-wei and transforming growth factor-β1(TGF-β1) and basic fibroblast growth factor(bFGF) in the aging human skin fibroblasts. Methods Firstly,to copy the aging model of human skin fibroblasts by continuous passage culturing.Secondly,to detect the c-Jun gene expression of the aging human skin fibroblasts through the fluorescent quantitative PCR method,after neutralizing antibody blocking TGF-β1 and bFGF. Results When the human skin fibroblasts aging,the expressive level of c-Jun gene,compared to that of young skin fibroblasts,falls by 70%and 71%in 24h and 48h.But theexpressive levels in 72h are both similar.As aging human skin fibroblasts treated by the recipe of supplementing Ying-wei,the c-Jun gene expression which has increased to 4.67 times and 2.12 times in 24h and 48h,has significantly improved,compared to the aging human skin fibroblasts.And the expressive levels of them are equivalent in 72h.After neutralizing TGF-β1 protein,c-Jun gene expressive level of the aging human skin fibroblasts is 2.4 times and 1.7 times as large as that of young skin fibroblasts in 24h and 72h.In 48h,the gene expressive level of young skin fibroblasts is 9.2 times as large as that of aging human skin fibroblasts.After using the recipe of supplementing Ying-wei,the c-Jun gene expression of aging human skin fibroblasts has improved obviously.The c-Jun gene expressive level of human skin fibroblasts raise by 2.3 times and 1.5 times in 48h and 72h.After neutralizing TGF-β1 protein and bFGF protein,c-Jun gene expressive level is decreased obviously with different time;Compared with the young skin fibroblasts,c-Jun gene expression of the aging human skin fibroblasts falls by 64.5%,94.2%and 73.7%in 24h,48h and 72h.As aging human skin fibroblasts treated by the recipe of supplementing Ying-wei,c-Jun gene expression has significantly increased over time.c-Jun gene expression in 24h,48h and 72h is of 9.7 times,3.6 times and 9.8 times as much as that of aging human skin fibroblasts.From the perspective of longitudinal comparison,TGF-β1 protein has a complex regulative effect on c-Jun gene expression of the aging human skin fibroblasts.And bFGF protein has obviously promoted the c-Jun gene expression of the aging human skin fibroblasts. Conclusion When people aging,the c-Jun gene expression of the aging human skin fibroblasts is disorder.The recipe of supplementing Ying-wei can promote the c-Jun gene expression of the aging human skin fibroblasts;And this promotion does not depend on the access of the TGF-β1 and bFGF.bFGF protein is the downstream protein of signal regulating pathways of the TGF-β1;and is one of the important promoting factors of c-Jun gene expression.