北京大学学报（医学版）
北京大學學報（醫學版）
북경대학학보（의학판）
Journal of Peking University (Health Sciences)
2015年
5期
733-736
,共4页
微RNA-29 b%肌萎缩侧索硬化%早期诊断
微RNA-29 b%肌萎縮側索硬化%早期診斷
미RNA-29 b%기위축측색경화%조기진단
MicroRNA-29b%Amyotrophic lateral sclerosis%Early diagnosis
目的：探讨microRNA-29b （miR-29b）在肌萎缩侧索硬化（amyotrophic lateral sclerosis，ALS）中的表达水平及其在该病诊断中的可能价值。方法：留取16只SOD1-G93A ALS模型鼠和16只野生型鼠脑皮质、脊髓、前肢肌肉组织和血浆，提取microRNA，采用实时荧光定量聚合酶链反应（ real-time quantitative polymerase chain reaction ，RT-qPCR）方法检测miR-29b的表达量，并通过受试者工作特征曲线（ receiver operator characteristic curve ，ROC）评估SOD1-G93A ALS模型鼠miR-29b对于ALS的诊断价值。结果：以U6 snRNA为内参，实验组SOD1-G93A ALS模型鼠脑皮质miR-29b相对表达量显著高于对照组水平（P＝0．001）。按周龄分组，8、12和16周龄实验组SOD1-G93A ALS模型鼠脑皮质miR-29b的相对表达量显著高于对照组水平（3个不同周龄的实验组vs．对照组显著性检验分别为P＝0．044、P＝0．018、P＝0．045）。通过SOD1-G93A ALS模型鼠脑皮质miR-29b的相对表达量（以U6 snRNA为内参）对ALS进行诊断时，ROC曲线下面积（area under the curve，AUC）为0．885，如果以0．1856为诊断临界值，灵敏度和特异度均较高，分别为92．9％和71．4％。结论：miR-29b可能会成为早期诊断ALS的检测指标。
目的：探討microRNA-29b （miR-29b）在肌萎縮側索硬化（amyotrophic lateral sclerosis，ALS）中的錶達水平及其在該病診斷中的可能價值。方法：留取16隻SOD1-G93A ALS模型鼠和16隻野生型鼠腦皮質、脊髓、前肢肌肉組織和血漿，提取microRNA，採用實時熒光定量聚閤酶鏈反應（ real-time quantitative polymerase chain reaction ，RT-qPCR）方法檢測miR-29b的錶達量，併通過受試者工作特徵麯線（ receiver operator characteristic curve ，ROC）評估SOD1-G93A ALS模型鼠miR-29b對于ALS的診斷價值。結果：以U6 snRNA為內參，實驗組SOD1-G93A ALS模型鼠腦皮質miR-29b相對錶達量顯著高于對照組水平（P＝0．001）。按週齡分組，8、12和16週齡實驗組SOD1-G93A ALS模型鼠腦皮質miR-29b的相對錶達量顯著高于對照組水平（3箇不同週齡的實驗組vs．對照組顯著性檢驗分彆為P＝0．044、P＝0．018、P＝0．045）。通過SOD1-G93A ALS模型鼠腦皮質miR-29b的相對錶達量（以U6 snRNA為內參）對ALS進行診斷時，ROC麯線下麵積（area under the curve，AUC）為0．885，如果以0．1856為診斷臨界值，靈敏度和特異度均較高，分彆為92．9％和71．4％。結論：miR-29b可能會成為早期診斷ALS的檢測指標。
목적：탐토microRNA-29b （miR-29b）재기위축측색경화（amyotrophic lateral sclerosis，ALS）중적표체수평급기재해병진단중적가능개치。방법：류취16지SOD1-G93A ALS모형서화16지야생형서뇌피질、척수、전지기육조직화혈장，제취microRNA，채용실시형광정량취합매련반응（ real-time quantitative polymerase chain reaction ，RT-qPCR）방법검측miR-29b적표체량，병통과수시자공작특정곡선（ receiver operator characteristic curve ，ROC）평고SOD1-G93A ALS모형서miR-29b대우ALS적진단개치。결과：이U6 snRNA위내삼，실험조SOD1-G93A ALS모형서뇌피질miR-29b상대표체량현저고우대조조수평（P＝0．001）。안주령분조，8、12화16주령실험조SOD1-G93A ALS모형서뇌피질miR-29b적상대표체량현저고우대조조수평（3개불동주령적실험조vs．대조조현저성검험분별위P＝0．044、P＝0．018、P＝0．045）。통과SOD1-G93A ALS모형서뇌피질miR-29b적상대표체량（이U6 snRNA위내삼）대ALS진행진단시，ROC곡선하면적（area under the curve，AUC）위0．885，여과이0．1856위진단림계치，령민도화특이도균교고，분별위92．9％화71．4％。결론：miR-29b가능회성위조기진단ALS적검측지표。
Objective:To investigate microRNA-29b ( miR-29b) expression in cerebral cortex , spinal cord, fore limb muscle, and serum of SOD1-G93A amyotrophic lateral sclerosis ( ALS) mice, and to identify the biomarker and to assess diagnostic values for ALS .Methods:Cerebral cortex , spinal cord , fore limb muscle and serum from 16 SOD1-G93 A ALS mice and 16 wild-type mice were taken and then microRNA extracted , detecting the expression of miR-29 b by real-time quantitative polymerase chain re-action ( RT-qPCR ) .The diagnostic performance of miR-29b for ALS was estimated by the receiver operating characteristic ( ROC ) curve . Results: The results from the validation indicated that the differences in miR-29b between the cerebral cortex of SOD1-G93A ALS and the healthy control subjects were statistically significant (P=0.001).Meanwhile, the expressions 8, 12, and 16 weeks later were higher than those of the controls ( ALS vs.Control: 8 weeks, P=0.044; 12 weeks, P=0.018; 16 weeks, P=0.045).When the relative expression level of miR-29b was used to diagnose ALS in SOD1-G93A ALS mice, the area under the ROC (area under the curve, AUC) was 0.885, if the diagnostic threshold was set at 0.185 6, the sensitivity and specificity were 92.9%and 71.4%.Conclusion:MiR-29 b may act as medical monitoring indices of ALS in early time .
