CAJ | 학술논문

背景：变应性鼻炎是指特应性个体接触变应原后，主要由 IgE 介导的介质组胺释放，并有多种免疫活性细胞和细胞因子等参与的鼻黏膜非感染性炎性疾病，与Th1和Th2免疫失衡相关。干扰素γ是Th1细胞分泌的细胞因子，而白细胞介素4是Th2细胞分泌的细胞因子。目的：构建稳定的129Sv小鼠变应性鼻炎模型，为129Sv小鼠为背景来源的基因敲除小鼠构建实验变应性鼻炎模型奠定基础，观察IgE、白细胞介素4和干扰素γ浓度变化。方法：将小鼠24只随机分2组，模型组采用卵清蛋白腹腔注射致敏激发，建立小鼠变应性鼻炎模型，对照组腹腔注射 PBS。建模成功后用取小鼠鼻黏膜染色后评估鼻黏膜嗜酸性粒细胞、浆细胞浸润的病理变化。用ELISA法检测白细胞介素4和干扰素γ细胞因子水平和卵清蛋白特异性IgE抗体浓度。结果与结论：与对照组相比，ELISA法检测显示，模型组血清中卵清蛋白-IgE和白细胞介素4浓度明显升高，干扰素γ浓度显著降低(P <0.05)；苏木精-伊红染色显示，模型组纤毛倒伏，黏膜下层浆液腺增生明显，嗜酸性粒细胞、浆细胞浸润较明显。结果证实，实验成功构建实验变应性鼻炎小鼠模型，变应性鼻炎的发病机制与Th1/Th2失衡有关。
배경：변응성비염시지특응성개체접촉변응원후，주요유 IgE 개도적개질조알석방，병유다충면역활성세포화세포인자등삼여적비점막비감염성염성질병，여Th1화Th2면역실형상관。간우소γ시Th1세포분비적세포인자，이백세포개소4시Th2세포분비적세포인자。목적：구건은정적129Sv소서변응성비염모형，위129Sv소서위배경래원적기인고제소서구건실험변응성비염모형전정기출，관찰IgE、백세포개소4화간우소γ농도변화。방법：장소서24지수궤분2조，모형조채용란청단백복강주사치민격발，건립소서변응성비염모형，대조조복강주사 PBS。건모성공후용취소서비점막염색후평고비점막기산성립세포、장세포침윤적병리변화。용ELISA법검측백세포개소4화간우소γ세포인자수평화란청단백특이성IgE항체농도。결과여결론：여대조조상비，ELISA법검측현시，모형조혈청중란청단백-IgE화백세포개소4농도명현승고，간우소γ농도현저강저(P <0.05)；소목정-이홍염색현시，모형조섬모도복，점막하층장액선증생명현，기산성립세포、장세포침윤교명현。결과증실，실험성공구건실험변응성비염소서모형，변응성비염적발병궤제여Th1/Th2실형유관。
BACKGROUND:Atopic individuals predispose to alergic rhinitis after contacting with an alergen, which is mainly released by IgE-mediated histamine. Alergic rhinitis is a kind of non-infectious inflammatory disease that involves a variety of immune cels and cytokines and is related to Th1 and Th2 immune imbalance. Interferon γ is a cytokine secreted by Th1 cels, while interleukin-4 is a cytokine secreted by Th2 cels. OBJECTIVE:To establish a stable 129Sv mouse model of alergic rhinitis, so as to lay the foundation of establishing gene knockout 129Sv mouse models of alergic rhinitis, and to observe the concentration variation of IgE, interleukin-4 and interferon γ. METHODS: Twenty-four mice were randomly divided into 2 groups: model and control groups. Mice in the model group were sensitized by intraperitoneal injection of ovalbumin to establish mouse models of alergic rhinitis. Mice in the control group were subjected to intraperitoneal injection of PBS. After successful modeling, pathological changes of nasal eosinophils and plasmocyte infiltration were evaluated by nasal mucosa staining. Interleukin 4 and interferon γ levels and ovalbumin-specific IgE antibody concentration were detected by enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION:Enzyme-linked immunosorbent assay showed that compared with the control group, serum ovalbumin-IgE and interleukin-4 concentrations were significantly increased, and Interferon γ concentration was significantly lower in the model group (P < 0.05). Hematoxylin-eosin staining results showed that cilia lodging and submucosal serous gland hyperplasia, eosinophils and plasmocyte infiltration were obvious. These results confirm that a mouse model of allergic rhinitis was successfully constructed in this study and the pathogenesis of allergic rhinitis was related to Th1/Th2 imbalance.