CAJ | 학술논문

背景：研究发现，去除坐骨神经会导致骨折愈合过程中新生编织骨机械硬度不足，而目前对有关失神经因素在牵张成骨过程中作用的相关报道较少。<br>　　目的：构建新西兰大白兔胫骨牵张成骨模型，观察失坐骨神经对牵张成骨过程中新骨形成的影响及Runx2表达的变化。<br>　　方法：成年新西兰雄性白兔24只建立兔胫骨牵张成骨延长模型，并随机分为切除坐骨神经组和保留左坐骨神经组。完成牵张6周后，用X射线、骨密度及三维CT重建技术检测已延长的胫骨，组织学分析新骨形成情况。结果与结论：影像学、组织学检查显示所有实验动物的牵张裂隙中均可发现有新骨生成，Runx2均有不同程度的表达。但与保留左坐骨神经组相比，切除坐骨神经组牵张裂隙中新生骨量较少、矿化程度低，并且Runx2表达量较低。提示失神经支配抑制牵张成骨中新骨的生成及Runx2的表达，神经支配可能在牵张成骨的新骨生成过程中起重要作用。
배경：연구발현，거제좌골신경회도치골절유합과정중신생편직골궤계경도불족，이목전대유관실신경인소재견장성골과정중작용적상관보도교소。<br>　　목적：구건신서란대백토경골견장성골모형，관찰실좌골신경대견장성골과정중신골형성적영향급Runx2표체적변화。<br>　　방법：성년신서란웅성백토24지건립토경골견장성골연장모형，병수궤분위절제좌골신경조화보류좌좌골신경조。완성견장6주후，용X사선、골밀도급삼유CT중건기술검측이연장적경골，조직학분석신골형성정황。결과여결론：영상학、조직학검사현시소유실험동물적견장렬극중균가발현유신골생성，Runx2균유불동정도적표체。단여보류좌좌골신경조상비，절제좌골신경조견장렬극중신생골량교소、광화정도저，병차Runx2표체량교저。제시실신경지배억제견장성골중신골적생성급Runx2적표체，신경지배가능재견장성골적신골생성과정중기중요작용。
BACKGROUND:During the healing of fractures, removal of sciatic nerve can result in insufficient mechanical rigidity of newborn woven bone. However, there are less reports concerning the denervation effects during distraction osteogenesis. OBJECTIVE:To observe the effect of removal of the sciatic nerve on bone regeneration and the expression of Runt-related transcription factor 2 (Runx2) protein during distraction osteogenesis in a rabbit model. METHODS:Twenty-four adult male New Zealand rabbits were selected and underwent left tibial osteodistraction to construct animal models of distraction osteogenesis. Before distraction, the animals were randomly divided into group R (resecting the left sciatic nerve) and group I (intact left sciatic nerve). Six weeks after completion of distraction, the animals were kil ed and the lengthened tibias were harvested for radiography, three-dimensional CT reconstruction, histological evaluation, connectivity density (Conn.D) evaluation. RESULTS AND CONCLUSION:New regenerated bone was present and Runx2 protein was expressed in the distraction gaps of al animals at the end of the study, as revealed by radiography, three-dimensional CT reconstruction, and histological observation. However, less new bone formation and a lower degree of mineralization and expression of Runx2 protein were observed in group R compared with group I. The results suggest that the denervation appears to have an inhibitory effect on bone formation and the expression of Runx2 protein during distraction osteogenesis.