CAJ | 학술논문

背景：研究发现，无敌丹能够抑制病变关节局部的炎症反应，保护关节软骨。 　　目的：验证无敌丹对家兔软骨细胞活力和软骨细胞凋亡的影响及对家兔骨关节炎病变的治疗效果。 　　方法：用含无敌丹的血清培养基培养大鼠软骨细胞，通过与溶剂对照组对比，观察无敌丹对软骨细胞活力及凋亡的影响；采用改良 Hulth 法构建兔膝骨关节炎模型，无敌丹给药组给予无敌丹；溶剂对照组给予生理盐水，2次/d，连续给药4周。观察无敌丹对兔膝骨关节炎的治疗作用；镜下观察关节局部的软骨细胞情况；用免疫组织化学的方法检测软骨中白细胞介素1、基质金属蛋白酶3的水平。 　　结果与结论：无敌丹血清培养的软骨细胞凋亡率显著低于对照组；兔膝关节病理检查显示溶剂对照组软骨破坏程度较高，无敌丹组软骨破坏程度低；免疫组织化学染色显示无敌丹给药组胞质和胞外基质着色浅，阳性细胞数量少，白细胞介素1表达低；无敌丹给药组阳性表达的细胞数量少，着色浅，基质金属蛋白酶3表达被抑制。结果表明，无敌丹能够有效保护家兔骨关节炎病变部位关节软骨，减少炎症反应，对于骨关节炎有很好的治疗作用，其机制可能与抑制软骨细胞凋亡、减少细胞因子的生成及抑制基质金属蛋白酶的蛋白表达有关。
배경：연구발현，무활단능구억제병변관절국부적염증반응，보호관절연골。 　　목적：험증무활단대가토연골세포활력화연골세포조망적영향급대가토골관절염병변적치료효과。 　　방법：용함무활단적혈청배양기배양대서연골세포，통과여용제대조조대비，관찰무활단대연골세포활력급조망적영향；채용개량 Hulth 법구건토슬골관절염모형，무활단급약조급여무활단；용제대조조급여생리염수，2차/d，련속급약4주。관찰무활단대토슬골관절염적치료작용；경하관찰관절국부적연골세포정황；용면역조직화학적방법검측연골중백세포개소1、기질금속단백매3적수평。 　　결과여결론：무활단혈청배양적연골세포조망솔현저저우대조조；토슬관절병리검사현시용제대조조연골파배정도교고，무활단조연골파배정도저；면역조직화학염색현시무활단급약조포질화포외기질착색천，양성세포수량소，백세포개소1표체저；무활단급약조양성표체적세포수량소，착색천，기질금속단백매3표체피억제。결과표명，무활단능구유효보호가토골관절염병변부위관절연골，감소염증반응，대우골관절염유흔호적치료작용，기궤제가능여억제연골세포조망、감소세포인자적생성급억제기질금속단백매적단백표체유관。
BACKGROUND:Studies have found that Wudi Dan can suppress local inflammatory response of the lesioned joints to protect the articular cartilage. OBJECTIVE:To verify the effect of Wudi Dan on chondrocyte viability and apoptosis as wel as the therapeutic effect on osteoarthritis. METHODS:Rat chondrocytes were cultured in serum medium containing Wudi Dan, and the effects of Wudi Dan on cel viability and apoptosis were observed by comparison with the control group. Rabbit model of knee osteoarthritis was constructed using modified Hulth method. Rabbit models were divided into two groups:Wudi Dan group treated with Wudi Dan and control group treated with normal saline, twice a day, consecutively for 4 weeks. Therapeutic effect of Wudi Dan on knee osteoarthritis was observed;cel viability and apoptosis were observed under microscope;the levels of interleukin-1 and matrix metal oproteinase-3 were determined using immunohistochemical method. RESULTS AND CONCLUSION:The apoptotic rate of chondrocytes was significantly lower in the Wudi Dan group than the control group. Pathological findings of the rabbit knee joints showed that the control group had more severe damage to the articular cartilage than the Wudi Dan group. Immunohistochemical staining revealed that in the Wudi Dan group, the cytoplasm and extracel ular matrix were colored light and there were a smal number of positive cel s as wel as low expression of interleukin-1 and matrix metal oproteinase-3. The results suggest that Wudi Dan can effectively protect against articular cartilage lesions, reduce inflammation, and have a good therapeutic effect on osteoarthritis. Its mechanism may be related to inhibition of chondrocyte apoptosis, reduction of cytokine production and inhibition of protein expression of matrix metal oproteinase.