中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
37期
5978-5982
,共5页
李雷激%徐超然%覃纲%刘跃华%祝琳
李雷激%徐超然%覃綱%劉躍華%祝琳
리뢰격%서초연%담강%류약화%축림
组织构建%组织工程%面神经损伤%神经型钙黏附分子%胎盘型钙黏附分子%面神经核%信号转导%面神经再生%免疫组织化学%实时定量PCR
組織構建%組織工程%麵神經損傷%神經型鈣黏附分子%胎盤型鈣黏附分子%麵神經覈%信號轉導%麵神經再生%免疫組織化學%實時定量PCR
조직구건%조직공정%면신경손상%신경형개점부분자%태반형개점부분자%면신경핵%신호전도%면신경재생%면역조직화학%실시정량PCR
背景：面神经周围性损伤后，首先涉及其中枢神经元轴突的逆行性反应，神经能否再生则取决于神经元胞体的存活及功能状态。<br> 目的：检测面神经损伤后，面神经核中神经型钙黏附分子和胎盘型钙黏附分子的表达变化。<br> 方法：将新西兰大白兔随机分为模型组(n=48)和对照组(n=8)。模型组兔建立右侧面神经压榨损伤模型。模型组分别于损伤后1，4，7，14，21，28 d各取8只兔进行检测。运用免疫组织化学SP法及实时定量PCR法检测兔右侧面神经核运动神经元中神经型钙黏附分子和胎盘型钙黏附分子蛋白及mRNA的表达水平。<br> 结果与结论：对照组兔右侧面神经核运动神经元中无神经型钙黏附分子或胎盘型钙黏附分子标记的阳性神经元。模型组兔右侧面神经核运动神经元中存在神经型钙黏附分子和胎盘型钙黏附分子阳性神经元，2种阳性神经元数量均在第14天时达到峰值。与对照组相比，模型组损伤后4-28 d兔面神经核中神经型钙黏附分子mRNA的表达水平明显增加，损伤后1 d时兔面神经核中胎盘型钙黏附分子mRNA的表达水平明显下降，损伤后7-28 d时兔面神经核中胎盘型钙黏附分子mRNA的表达水平明显增加。提示面神经损伤的早期即出现2种分子的阳性表达，其中胎盘型钙黏附分子的表达自神经损伤后一直存在，而神经型钙黏附分子表达时间相对较短。在面神经损伤时，面神经核中神经型钙黏附分子和胎盘型钙黏附分子均表达增加，说明面神经再生可能与黏附分子的高表达有关。
揹景：麵神經週圍性損傷後，首先涉及其中樞神經元軸突的逆行性反應，神經能否再生則取決于神經元胞體的存活及功能狀態。<br> 目的：檢測麵神經損傷後，麵神經覈中神經型鈣黏附分子和胎盤型鈣黏附分子的錶達變化。<br> 方法：將新西蘭大白兔隨機分為模型組(n=48)和對照組(n=8)。模型組兔建立右側麵神經壓榨損傷模型。模型組分彆于損傷後1，4，7，14，21，28 d各取8隻兔進行檢測。運用免疫組織化學SP法及實時定量PCR法檢測兔右側麵神經覈運動神經元中神經型鈣黏附分子和胎盤型鈣黏附分子蛋白及mRNA的錶達水平。<br> 結果與結論：對照組兔右側麵神經覈運動神經元中無神經型鈣黏附分子或胎盤型鈣黏附分子標記的暘性神經元。模型組兔右側麵神經覈運動神經元中存在神經型鈣黏附分子和胎盤型鈣黏附分子暘性神經元，2種暘性神經元數量均在第14天時達到峰值。與對照組相比，模型組損傷後4-28 d兔麵神經覈中神經型鈣黏附分子mRNA的錶達水平明顯增加，損傷後1 d時兔麵神經覈中胎盤型鈣黏附分子mRNA的錶達水平明顯下降，損傷後7-28 d時兔麵神經覈中胎盤型鈣黏附分子mRNA的錶達水平明顯增加。提示麵神經損傷的早期即齣現2種分子的暘性錶達，其中胎盤型鈣黏附分子的錶達自神經損傷後一直存在，而神經型鈣黏附分子錶達時間相對較短。在麵神經損傷時，麵神經覈中神經型鈣黏附分子和胎盤型鈣黏附分子均錶達增加，說明麵神經再生可能與黏附分子的高錶達有關。
배경：면신경주위성손상후，수선섭급기중추신경원축돌적역행성반응，신경능부재생칙취결우신경원포체적존활급공능상태。<br> 목적：검측면신경손상후，면신경핵중신경형개점부분자화태반형개점부분자적표체변화。<br> 방법：장신서란대백토수궤분위모형조(n=48)화대조조(n=8)。모형조토건립우측면신경압자손상모형。모형조분별우손상후1，4，7，14，21，28 d각취8지토진행검측。운용면역조직화학SP법급실시정량PCR법검측토우측면신경핵운동신경원중신경형개점부분자화태반형개점부분자단백급mRNA적표체수평。<br> 결과여결론：대조조토우측면신경핵운동신경원중무신경형개점부분자혹태반형개점부분자표기적양성신경원。모형조토우측면신경핵운동신경원중존재신경형개점부분자화태반형개점부분자양성신경원，2충양성신경원수량균재제14천시체도봉치。여대조조상비，모형조손상후4-28 d토면신경핵중신경형개점부분자mRNA적표체수평명현증가，손상후1 d시토면신경핵중태반형개점부분자mRNA적표체수평명현하강，손상후7-28 d시토면신경핵중태반형개점부분자mRNA적표체수평명현증가。제시면신경손상적조기즉출현2충분자적양성표체，기중태반형개점부분자적표체자신경손상후일직존재，이신경형개점부분자표체시간상대교단。재면신경손상시，면신경핵중신경형개점부분자화태반형개점부분자균표체증가，설명면신경재생가능여점부분자적고표체유관。
BACKGROUND:Peripheral facial nerve injury first involves the retrograde reactions of central nervous system axons, and nerve regeneration wil depend on the survival and functional status of neuronal cel bodies. OBJECTIVE:To explore the expression of neuronal cadherin and placental cadherin in facial nuclei after facial nerve injury. METHODS:New Zealand white rabbits were randomly divided into model group (n=48) and control group (n=8). In the model group, every eight rabbits were used to carry out the test respectively at 1, 4, 7, 14, 21 days after the model of facial nerve injury (right side) was established. SP and real-time quantitative PCR methods were taken to test the expression of neuronal cadherin and placental cadherin in the facial nerve nucleus at mRNA and RESULTS AND CONCLUSION:The control group had neuronal cadherin-and placental cadherin-positive neurons. In the model group, neuronal cadherin and placental cadherin positively expressed in the facial motorneurons (right side), and their expressions were peaked at 14 days. Compared with the control group, the mRNA expression of neuronal cadherin in the facial motorneurons was increased significantly at 4-28 days after injury;the mRNA expression of placental cadherin in the facial motorneurons was decreased significantly at 1 day after injury, and then increased significantly at 7-28 days. It is suggested that the expression of neuronal cadherin and placental cadherin is positive in the early stage of facial nerve injury, and the expression of placental cadherin is always present, while the expression of neuronal cadherin relatively lasts for a short time. After facial nerve injury, the expression of neuronal cadherin and placental cadherin in the facial nerve nucleus is both increased, which indicates that the facial nerve regeneration may be related to the high expression of adhesion molecules.
