中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
40期
6509-6514
,共6页
实验动物%组织构建实验模型%苯丙酸诺龙%烫伤%类固醇受体辅助活化因子1%c-myc%胰岛素样生长因子1
實驗動物%組織構建實驗模型%苯丙痠諾龍%燙傷%類固醇受體輔助活化因子1%c-myc%胰島素樣生長因子1
실험동물%조직구건실험모형%분병산낙룡%탕상%류고순수체보조활화인자1%c-myc%이도소양생장인자1
背景：中重度烧创伤一直是临床全身治疗的难点，与创面局部处理并重，极大影响治疗预后。同化激素在烧伤动物模型与临床患者的使用治疗中取得安全良好效果。同化激素的应用虽受运动兴奋剂管理的限制，但其雄激素受体与核受体辅助调节因子仍是近年来基因调控机制研究的热门新兴领域。目的：探索苯丙酸诺龙对烫伤大鼠体内雄激素受体介导靶基因转录调控的影响。方法：将36只大鼠随机分为苯丙酸诺龙组、模型组与对照组。苯丙酸诺龙组和模型组大鼠以热水制成20%体表面积深Ⅱ度烫伤大鼠模型后2 d，分别后肢肌肉注射苯丙酸诺龙和生理盐水，隔日1次，连续21 d。结果与结论：苯丙酸诺龙组与模型组大鼠肝脏及性腺(睾丸、卵巢)中类固醇受体辅助活化因子1和胰岛素样生长因子1基因表达水平差异有显著性意义(P <0.05，除肝脏中类固醇受体辅助活化因子1)；而模型组与对照组大鼠肝脏及性腺(睾丸、卵巢)中类固醇受体辅助活化因子1、c-myc和胰岛素样生长因子1基因表达水平差异无显著性意义(P >0.05)。说明在不同组织不同生理病理条件下苯丙酸诺龙对类固醇受体辅助活化因子1、c-myc、胰岛素样生长因子1基因表达的作用是不同的。
揹景：中重度燒創傷一直是臨床全身治療的難點，與創麵跼部處理併重，極大影響治療預後。同化激素在燒傷動物模型與臨床患者的使用治療中取得安全良好效果。同化激素的應用雖受運動興奮劑管理的限製，但其雄激素受體與覈受體輔助調節因子仍是近年來基因調控機製研究的熱門新興領域。目的：探索苯丙痠諾龍對燙傷大鼠體內雄激素受體介導靶基因轉錄調控的影響。方法：將36隻大鼠隨機分為苯丙痠諾龍組、模型組與對照組。苯丙痠諾龍組和模型組大鼠以熱水製成20%體錶麵積深Ⅱ度燙傷大鼠模型後2 d，分彆後肢肌肉註射苯丙痠諾龍和生理鹽水，隔日1次，連續21 d。結果與結論：苯丙痠諾龍組與模型組大鼠肝髒及性腺(睪汍、卵巢)中類固醇受體輔助活化因子1和胰島素樣生長因子1基因錶達水平差異有顯著性意義(P <0.05，除肝髒中類固醇受體輔助活化因子1)；而模型組與對照組大鼠肝髒及性腺(睪汍、卵巢)中類固醇受體輔助活化因子1、c-myc和胰島素樣生長因子1基因錶達水平差異無顯著性意義(P >0.05)。說明在不同組織不同生理病理條件下苯丙痠諾龍對類固醇受體輔助活化因子1、c-myc、胰島素樣生長因子1基因錶達的作用是不同的。
배경：중중도소창상일직시림상전신치료적난점，여창면국부처리병중，겁대영향치료예후。동화격소재소상동물모형여림상환자적사용치료중취득안전량호효과。동화격소적응용수수운동흥강제관리적한제，단기웅격소수체여핵수체보조조절인자잉시근년래기인조공궤제연구적열문신흥영역。목적：탐색분병산낙룡대탕상대서체내웅격소수체개도파기인전록조공적영향。방법：장36지대서수궤분위분병산낙룡조、모형조여대조조。분병산낙룡조화모형조대서이열수제성20%체표면적심Ⅱ도탕상대서모형후2 d，분별후지기육주사분병산낙룡화생리염수，격일1차，련속21 d。결과여결론：분병산낙룡조여모형조대서간장급성선(고환、란소)중류고순수체보조활화인자1화이도소양생장인자1기인표체수평차이유현저성의의(P <0.05，제간장중류고순수체보조활화인자1)；이모형조여대조조대서간장급성선(고환、란소)중류고순수체보조활화인자1、c-myc화이도소양생장인자1기인표체수평차이무현저성의의(P >0.05)。설명재불동조직불동생리병리조건하분병산낙룡대류고순수체보조활화인자1、c-myc、이도소양생장인자1기인표체적작용시불동적。
BACKGROUND:Moderate to severe burn and trauma, treatment of which has been paid equal attention to wound surface, is always a difficulty of clinical systemic treatment and has a poor prognosis. Anabolic hormones have acquired secure and good results in the treatment of burns in both animals and clinical patients. Although use of anabolic hormones is restricted by the doping management, but its androgen receptor and nuclear receptor coregulators are the newly emerging areas of interests in the field of gene regulation mechanism in recent years. OBJECTIVE:To investigate the effect of nandrolone phenylpropionate on androgen receptor-mediated transcriptional regulation of target gene in rat scald models. METHODS: Thirty-six rats were randomly divided into nandrolone phenylpropionate, model and control groups. In the nandrolone phenylpropionate and model groups, rats were subjected to a 20% total body surface area second-degree scald injury by hot water. Two days after model preparation, rats in these two groups were intramuscularly injected with nandrolone phenylpropionate and saline, once every other day, for a total of 21 days. RESULTS AND CONCLUSION:The gene expression levels of steroid receptor coactivator-1 and insulin-like growth factor 1 in the rat livers and gonads (testes, ovaries) were significantly different between the nandrolone phenylpropionate and model groups (P < 0.05), except steroid receptor coactivator-1 in the liver. There were no significant differences in steroid receptor coactivator-1, c-myc and insulin-like growth factor 1 in the rat livers and gonads (testes, ovaries) between model and control groups (P < 0.05). The results demonstrate that nandrolone phenylpropionate has different effects on the gene expression of steroid receptor coactivator-1, c-myc and insulin-like growth factor 1 in different tissues under different physiological and pathological conditions.
