中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
40期
6504-6508
,共5页
实验动物%口腔损伤及修复动物模型%复发性口疮%细胞凋亡%细胞外基质降解%重构%基质金属蛋白酶%黄芩苷%弗氏免疫佐剂
實驗動物%口腔損傷及脩複動物模型%複髮性口瘡%細胞凋亡%細胞外基質降解%重構%基質金屬蛋白酶%黃芩苷%弗氏免疫佐劑
실험동물%구강손상급수복동물모형%복발성구창%세포조망%세포외기질강해%중구%기질금속단백매%황금감%불씨면역좌제
背景：细胞外基质的降解与重构是多种病理及生理过程的共同通路，参与了多种口腔疾病的发病过程。目的：分析复发性口疮大鼠细胞凋亡的细胞外基质重构参与作用及黄芩苷的干预作用，为相关新药开发和临床治疗提供参考。方法：40只SD大鼠随机分为4组：对照组、复发性口疮模型组、小剂量黄芩苷组和大剂量黄芩苷组。后3组大鼠采用口腔黏膜匀浆组织加弗氏免疫佐剂建立大鼠复发性口疮模型。小剂量黄芩苷组和大剂量黄芩苷组大鼠分别给予10 mg/kg和100 mg/kg黄芩苷灌胃，1次/d，连续14 d。结果与结论：与对照组相比，模型组大鼠血清超氧化物歧化酶水平明显降低，而丙二醛水平明显增高，口腔黏膜中Bax、Caspase3和基质金属蛋白酶2/9表达水平增加，而Bcl-2表达水平降低。而小剂量黄芩苷组和大剂量黄芩苷组大鼠上述指标明显恢复，且不同剂量黄芩苷的影响差异有显著性意义。提示黄芩苷主要通过抑制细胞外基质降解与重构过程治疗复发性口疮。
揹景：細胞外基質的降解與重構是多種病理及生理過程的共同通路，參與瞭多種口腔疾病的髮病過程。目的：分析複髮性口瘡大鼠細胞凋亡的細胞外基質重構參與作用及黃芩苷的榦預作用，為相關新藥開髮和臨床治療提供參攷。方法：40隻SD大鼠隨機分為4組：對照組、複髮性口瘡模型組、小劑量黃芩苷組和大劑量黃芩苷組。後3組大鼠採用口腔黏膜勻漿組織加弗氏免疫佐劑建立大鼠複髮性口瘡模型。小劑量黃芩苷組和大劑量黃芩苷組大鼠分彆給予10 mg/kg和100 mg/kg黃芩苷灌胃，1次/d，連續14 d。結果與結論：與對照組相比，模型組大鼠血清超氧化物歧化酶水平明顯降低，而丙二醛水平明顯增高，口腔黏膜中Bax、Caspase3和基質金屬蛋白酶2/9錶達水平增加，而Bcl-2錶達水平降低。而小劑量黃芩苷組和大劑量黃芩苷組大鼠上述指標明顯恢複，且不同劑量黃芩苷的影響差異有顯著性意義。提示黃芩苷主要通過抑製細胞外基質降解與重構過程治療複髮性口瘡。
배경：세포외기질적강해여중구시다충병리급생리과정적공동통로，삼여료다충구강질병적발병과정。목적：분석복발성구창대서세포조망적세포외기질중구삼여작용급황금감적간예작용，위상관신약개발화림상치료제공삼고。방법：40지SD대서수궤분위4조：대조조、복발성구창모형조、소제량황금감조화대제량황금감조。후3조대서채용구강점막균장조직가불씨면역좌제건립대서복발성구창모형。소제량황금감조화대제량황금감조대서분별급여10 mg/kg화100 mg/kg황금감관위，1차/d，련속14 d。결과여결론：여대조조상비，모형조대서혈청초양화물기화매수평명현강저，이병이철수평명현증고，구강점막중Bax、Caspase3화기질금속단백매2/9표체수평증가，이Bcl-2표체수평강저。이소제량황금감조화대제량황금감조대서상술지표명현회복，차불동제량황금감적영향차이유현저성의의。제시황금감주요통과억제세포외기질강해여중구과정치료복발성구창。
BACKGROUND:Extracelular matrix degradation and remodeling is the common pathway of a variety of pathological and physiological processes, and is invovled in the pathogenesis of a variety of oral diseases. OBJECTIVE: To analyze the role of extracelular matrix remodeling folowing celular apoptosis in recurrent aphthous ulcer and the therapeutic effect of baicalin, so as to provide references for new drug development and clinical treatment. METHODS: Forty Sprague-Dawley rats were randomly divided into four groups: control, recurrent aphthous ulcer model, low-dose baicalin and high-dose baicalin groups. Rat models were established using oral mucosal homogenate plus Freund’s immunoadjuvants in the recurrent aphthous ulcer model, low-dose baicalin, and high-dose baicalin groups. Rats in low-dose baicalin and high-dose baicalin groups were intragastricaly administered 10 mg/kg and 100 mg/kg of baicalin respectively for 14 days, once per day. RESULTS AND CONCLUSION:Compared with the control group, serum superoxide dismutase level was distinctly decreased, however, malondialdehyde level distinctly increased; the expression levels of Bax, Caspase3 and matrix metaloproteinase 2/9 were increased, but the expression of Bcl2 was decreased in the recurrent aphthous ulcer model group. The abovementioned indicators of rats returned greatly towards normal levels in the low dose baicalin and high dose baicalin groups, and there was significant difference in the effects of different doses of baicalin on these indicators. These results suggest that baicalin treats recurrent aphthous ulcer mainly through inhibiting extracelular matrix degradation and remodeling.
