安徽农学通报
安徽農學通報
안휘농학통보
Anhui Agricultural Science Bulletin
2015年
19期
25-28
,共4页
陈挺%娄永江%庞林江%王茜茜
陳挺%婁永江%龐林江%王茜茜
진정%루영강%방림강%왕천천
年糕%分离纯化%微生物%鉴定
年糕%分離純化%微生物%鑒定
년고%분리순화%미생물%감정
Rice cakes%Separation and purification%Microbes%Identification
通过对年糕内部和表面的微生物进行分离纯化,并通过形态观察、生化测定的方法来鉴定微生物的组成,结果表明:年糕表面的微生物主要有6种细菌、3种霉菌和2种酵母菌。细菌中4种是芽孢杆菌,分别为巨大芽孢杆菌(Bacillus megaterium)、枯草芽孢杆菌(Bacillus subtilis)、蜡状芽孢杆菌(Bacillus cereus)和短芽孢杆菌(Bacillus brevis),2种是非芽孢杆菌,分别为乙酰短杆菌(Brevibacterium acetylicum)和乙酸钙不动杆菌(Acinetobacter calcoaceticus);霉菌分别为米根霉(Rhizopus oryzae)、构巢曲霉(Aspergillus nidulans)和米曲霉(As?pergillus oryzae);酵母菌分别为茁芽丝孢酵母(Trichosporon pullulans)和白色假丝酵母(Candida albicans)。年糕内部的微生物有巨大芽孢杆菌、枯草芽孢杆菌、短芽孢杆菌和白色假丝酵母,无霉菌。
通過對年糕內部和錶麵的微生物進行分離純化,併通過形態觀察、生化測定的方法來鑒定微生物的組成,結果錶明:年糕錶麵的微生物主要有6種細菌、3種黴菌和2種酵母菌。細菌中4種是芽孢桿菌,分彆為巨大芽孢桿菌(Bacillus megaterium)、枯草芽孢桿菌(Bacillus subtilis)、蠟狀芽孢桿菌(Bacillus cereus)和短芽孢桿菌(Bacillus brevis),2種是非芽孢桿菌,分彆為乙酰短桿菌(Brevibacterium acetylicum)和乙痠鈣不動桿菌(Acinetobacter calcoaceticus);黴菌分彆為米根黴(Rhizopus oryzae)、構巢麯黴(Aspergillus nidulans)和米麯黴(As?pergillus oryzae);酵母菌分彆為茁芽絲孢酵母(Trichosporon pullulans)和白色假絲酵母(Candida albicans)。年糕內部的微生物有巨大芽孢桿菌、枯草芽孢桿菌、短芽孢桿菌和白色假絲酵母,無黴菌。
통과대년고내부화표면적미생물진행분리순화,병통과형태관찰、생화측정적방법래감정미생물적조성,결과표명:년고표면적미생물주요유6충세균、3충매균화2충효모균。세균중4충시아포간균,분별위거대아포간균(Bacillus megaterium)、고초아포간균(Bacillus subtilis)、사상아포간균(Bacillus cereus)화단아포간균(Bacillus brevis),2충시비아포간균,분별위을선단간균(Brevibacterium acetylicum)화을산개불동간균(Acinetobacter calcoaceticus);매균분별위미근매(Rhizopus oryzae)、구소곡매(Aspergillus nidulans)화미곡매(As?pergillus oryzae);효모균분별위촬아사포효모(Trichosporon pullulans)화백색가사효모(Candida albicans)。년고내부적미생물유거대아포간균、고초아포간균、단아포간균화백색가사효모,무매균。
Through the morphology observation and the physiological and biochemical of microorganisms which were separated and purified from the interior and the surface of the rice cake,we can get that there were 6 kinds of bacteri?um(involving 4 kinds of Bacillus which were Bacillus megaterium,Bacillus subtilis,Bacillus cereus and Bacillus bre?vis,2 kinds of non-Bacillus were Brevibacterium acetylicum and Acinetobacter calcoaceticus),3 kinds of molds(Rhizo?pus oryzae,Aspergillus oryzae and Aspergillus nidulans),and 2 kinds of Yeast(they were respectively Candida albi?cans and Trichosporon pullulans)on the surface of rice cake.The inside microorganisms of Rice cake were Bacillus megaterium,Bacillus subtilis,Bacillus brevis and trichosporon pullulans without mold.