CAJ | 학술논문

采用超声波辅助提取辣木叶总黄酮，并利用响应面法对提取工艺参数进行优化。首先通过单因素试验分别考察超声波提取的料液比、提取时间、提取温度和提取功率对辣木叶总黄酮提取率的影响，确定各因素的适宜水平；在此基础上进行四因素三水平的 Box-Behnken 中心组合设计法设计响应面试验，分析不同因素、不同水平对辣木叶总黄酮得率的影响。超声辅助提取辣木叶总黄酮的最佳提取工艺条件为：料液比1∶35（g/mL）、提取时间40 min、提取温度52℃、功率210 W，在此条件下辣木叶总黄酮提取率的预测值为5.082%，验证试验所得总黄酮含量为5.17%，所得回归模型拟合情况良好。
채용초성파보조제취랄목협총황동，병이용향응면법대제취공예삼수진행우화。수선통과단인소시험분별고찰초성파제취적료액비、제취시간、제취온도화제취공솔대랄목협총황동제취솔적영향，학정각인소적괄의수평；재차기출상진행사인소삼수평적 Box-Behnken 중심조합설계법설계향응면시험，분석불동인소、불동수평대랄목협총황동득솔적영향。초성보조제취랄목협총황동적최가제취공예조건위：료액비1∶35（g/mL）、제취시간40 min、제취온도52℃、공솔210 W，재차조건하랄목협총황동제취솔적예측치위5.082%，험증시험소득총황동함량위5.17%，소득회귀모형의합정황량호。
Response surface methodology(RSM)was used to optimize the experimental conditions for ultrasonic-assisted ex-traction of total flavonoids from Moringa oleifera leaves. First, the effect of solid-to-liquid ratio, extraction time, extraction temperature, and extraction power on the extraction yield of total flavonoids were investigated using single factor experiment. Based on the results, the experimental conditions were optimized using a four-variable, three-level Box-Behnken experimental design, and the optimum conditions for the extraction were obtained as follows: 1:35(g/mL)for the solid-to-liquid ratio, 40 min for the extraction time, 52℃ for the extraction temperature, and 210 W for the extraction power. Under the optimum condi-tions, the predicted and actual yields of total flavonoids were 5.082% and 5.17%. The model developed by RSM was adequate to describe the relationships between the factors and the response values..