中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
Chinese Journal of Immunology
2015年
10期
1310-1314
,共5页
王健%赵富玺%杨晶%刘润花%穆雅琴%王喜英%郭俊成%刘丽华%白光梅
王健%趙富璽%楊晶%劉潤花%穆雅琴%王喜英%郭俊成%劉麗華%白光梅
왕건%조부새%양정%류윤화%목아금%왕희영%곽준성%류려화%백광매
5-氮-2′-脱氧胞苷%CD4+CD25+Foxp3+调节性T细胞%过继转输%反复自然流产
5-氮-2′-脫氧胞苷%CD4+CD25+Foxp3+調節性T細胞%過繼轉輸%反複自然流產
5-담-2′-탈양포감%CD4+CD25+Foxp3+조절성T세포%과계전수%반복자연유산
5-aza-2′-deoxycytidine%CD4+CD25+Foxp3+Treg%Adoptivetherapy%Recurrentspontaneousabortion
目的:研究过继转输5-氮-2′-脱氧胞苷(5-aza-2′-deoxycytidine,5AzaD)诱导的 CD4+CD25+Foxp3+调节性T细胞(inducible regulatory T cells,iTregs)对自然流产孕鼠妊娠预后的影响。方法:以60例雌性CBA/J×雄性DBA/2J小鼠自然流产模型为研究对象,随机选取20例孕鼠经免疫磁珠分选脾脏CD4+T细胞,应用5AzaD对CD4+CD25-T细胞进行体外处理得到iTregs,通过流式细胞术检测表观修饰前后Tregs细胞中Foxp3的表达。分别向妊娠第1天和第4天的流产孕鼠体内输注iTregs作为治疗组,于妊娠14天比较各组孕鼠的胚胎吸收率。结果:经5AzaD修饰后,iTregs占CD4+T细胞的比例为(41.50±8.03)%。治疗组中妊娠第1天和第4天过继转输iTregs孕鼠的胎盘吸收率分别为10.47%和21.69%(P<0.05)。结论:对CD4+CD25-T细胞的表观修饰可能解决自然调节性T细胞来源不足的问题,在妊娠早期转输5AzaD诱导的iTreg细胞更有利于维持正常妊娠。
目的:研究過繼轉輸5-氮-2′-脫氧胞苷(5-aza-2′-deoxycytidine,5AzaD)誘導的 CD4+CD25+Foxp3+調節性T細胞(inducible regulatory T cells,iTregs)對自然流產孕鼠妊娠預後的影響。方法:以60例雌性CBA/J×雄性DBA/2J小鼠自然流產模型為研究對象,隨機選取20例孕鼠經免疫磁珠分選脾髒CD4+T細胞,應用5AzaD對CD4+CD25-T細胞進行體外處理得到iTregs,通過流式細胞術檢測錶觀脩飾前後Tregs細胞中Foxp3的錶達。分彆嚮妊娠第1天和第4天的流產孕鼠體內輸註iTregs作為治療組,于妊娠14天比較各組孕鼠的胚胎吸收率。結果:經5AzaD脩飾後,iTregs佔CD4+T細胞的比例為(41.50±8.03)%。治療組中妊娠第1天和第4天過繼轉輸iTregs孕鼠的胎盤吸收率分彆為10.47%和21.69%(P<0.05)。結論:對CD4+CD25-T細胞的錶觀脩飾可能解決自然調節性T細胞來源不足的問題,在妊娠早期轉輸5AzaD誘導的iTreg細胞更有利于維持正常妊娠。
목적:연구과계전수5-담-2′-탈양포감(5-aza-2′-deoxycytidine,5AzaD)유도적 CD4+CD25+Foxp3+조절성T세포(inducible regulatory T cells,iTregs)대자연유산잉서임신예후적영향。방법:이60례자성CBA/J×웅성DBA/2J소서자연유산모형위연구대상,수궤선취20례잉서경면역자주분선비장CD4+T세포,응용5AzaD대CD4+CD25-T세포진행체외처리득도iTregs,통과류식세포술검측표관수식전후Tregs세포중Foxp3적표체。분별향임신제1천화제4천적유산잉서체내수주iTregs작위치료조,우임신14천비교각조잉서적배태흡수솔。결과:경5AzaD수식후,iTregs점CD4+T세포적비례위(41.50±8.03)%。치료조중임신제1천화제4천과계전수iTregs잉서적태반흡수솔분별위10.47%화21.69%(P<0.05)。결론:대CD4+CD25-T세포적표관수식가능해결자연조절성T세포래원불족적문제,재임신조기전수5AzaD유도적iTreg세포경유리우유지정상임신。
Objective:To investigate the effect of the adoptive transfer of CD4+CD25+Foxp3+regulatory T cells ( iTregs) induced by 5-aza-2′-deoxycytidine (5AzaD) on pregnant outcome of the abortion-prone mice.Methods:Sixty cases of female CBA/J × male DBA/2J abortion-prone matings were taken as study group,the CD4+T cells from spleen of twenty female CBA/J mice were separated by magnetic activated cell sorting (MACS),5AzaD was applied to the conversion of CD4+CD25-T cells to iTregs,the expression of Foxp3 in Tregs was characterized by flow cytometry analysis before and after epigenetic modification.The purified iTregs were injected into abortion-prone mice on day 1 or 4 of pregnancy,respectively,which were used as therapy groups,and then the embryo resorption rate was counted on day 14 of pregnancy.Results:After the treatment of 5AzaD,the percentage of iTregs in CD4+T cells was (41.50±8.03)%.The embryonic absorption rates of the two therapy groups were 10.47%(on day 1 of pregnancy) and 21.69%(on day 4 of pregnancy) ,respectively ( P<0.05 ) .Conclusion: Epigenetic modication of CD4+CD25-T cells may solve the problem of nTregs deficiency,particularly adoptive therapy of 5AzaD-induced iTregs at early stage of pregnancy can maintain normal pregnancy.