植物营养与肥料学报
植物營養與肥料學報
식물영양여비료학보
Plant Nutrition and Fertilizer Science
2015年
5期
1361-1365
,共5页
李灿东%郭泰%王志新%郑伟%张振宇%李于%王囡囡%刘忠堂
李燦東%郭泰%王誌新%鄭偉%張振宇%李于%王囡囡%劉忠堂
리찬동%곽태%왕지신%정위%장진우%리우%왕닙닙%류충당
大豆%叶面%氮素%吸收与分配
大豆%葉麵%氮素%吸收與分配
대두%협면%담소%흡수여분배
soybean%leaf%nitrogen%uptake and distribution
【目的】叶面喷施氮肥在大豆生产中已普遍应用,大量研究报道表明叶面喷施氮肥能够使大豆获得不同程度的增产。本研究在前人研究的基础上,采用15 N示踪技术,探索不同施氮量下氮素经大豆叶面吸收后在大豆植株各组织器官的积累与分配情况,为大豆叶面氮肥的高效利用提供理论依据。【方法】在黑龙江省大豆优势产区三江平原,以该地区5年内推广种植面积最大的大豆主栽品种“合丰55”为试验材料,采用15 N示踪技术,以上海化工研究院生产的丰度为20.17%的15 N标记尿素水溶液为叶面肥料,设置不同叶面氮素喷施量处理N 0、3.5、4.0、4.5、5.0 kg/hm2(N0、N1、N2、N3、N4),在大豆重要的需氮时期鼓粒期(R5)进行叶面施氮处理。分析不同叶面氮素喷施量对大豆标记氮吸收、分配利用规律以及对产量的影响。【结果】叶面喷施N 4.5 kg/hm2( N3)大豆各器官干物质积累量、氮素含量及氮素积累量均显著高于其他处理(P<0.05)。与不施氮处理(N0)相比,籽粒干重(21.7 g/plant)和总干物重(70.1 g/plant)分别增加6.37%和8.51%,籽粒氮素含量(6.15 g/kg)增加10.81%,籽粒氮素积累量(133.3 mg/plant)增加18.07%。在同一施氮水平下,大豆不同器官标记N积累量为籽粒>茎>叶>荚皮>叶柄>根,差异达到显著水平( P<0.05)。在施氮量为4.5 kg/hm2处理条件下,籽粒标记氮积累量(9.76 mg/plant)分别较茎(2.46 mg/plant)、叶(1.28 mg/plant)、荚皮(1.26 mg/plant)、叶柄(0.9 mg/plant)及根(0.41 mg/plant)高2.96、6.63、6.75、9.84和22.8倍。不同施氮处理下,各器官标记氮积累量随着施氮量的增加呈先增加后降低的趋势,在施氮量为4.5 kg/hm2处理条件下达到最高值,其中籽粒中标记氮达到9.76 mg/plant。标记氮在各器官的分配比例与积累量无明显相关性,可能是不同施氮量下各器官干物质积累量不同所致,总体表现为籽粒>茎>叶>荚皮>叶柄>根,在施氮量为5.0 kg/hm2条件下籽粒标记氮分配率最高,为63.81%。【结论】在叶面喷施氮4.5 kg/hm2条件下,籽粒标记氮积累量和干物重最高,分别为每株9.76 mg和21.7 g。就“合丰55”品种而言,叶面施氮量为4.5 kg/hm2最有利于籽粒氮素及干物质积累。大豆鼓粒期( R5)进行叶面施氮时,氮素主要积累于籽粒中,有利于籽粒干物质积累,最终获得增产。
【目的】葉麵噴施氮肥在大豆生產中已普遍應用,大量研究報道錶明葉麵噴施氮肥能夠使大豆穫得不同程度的增產。本研究在前人研究的基礎上,採用15 N示蹤技術,探索不同施氮量下氮素經大豆葉麵吸收後在大豆植株各組織器官的積纍與分配情況,為大豆葉麵氮肥的高效利用提供理論依據。【方法】在黑龍江省大豆優勢產區三江平原,以該地區5年內推廣種植麵積最大的大豆主栽品種“閤豐55”為試驗材料,採用15 N示蹤技術,以上海化工研究院生產的豐度為20.17%的15 N標記尿素水溶液為葉麵肥料,設置不同葉麵氮素噴施量處理N 0、3.5、4.0、4.5、5.0 kg/hm2(N0、N1、N2、N3、N4),在大豆重要的需氮時期鼓粒期(R5)進行葉麵施氮處理。分析不同葉麵氮素噴施量對大豆標記氮吸收、分配利用規律以及對產量的影響。【結果】葉麵噴施N 4.5 kg/hm2( N3)大豆各器官榦物質積纍量、氮素含量及氮素積纍量均顯著高于其他處理(P<0.05)。與不施氮處理(N0)相比,籽粒榦重(21.7 g/plant)和總榦物重(70.1 g/plant)分彆增加6.37%和8.51%,籽粒氮素含量(6.15 g/kg)增加10.81%,籽粒氮素積纍量(133.3 mg/plant)增加18.07%。在同一施氮水平下,大豆不同器官標記N積纍量為籽粒>莖>葉>莢皮>葉柄>根,差異達到顯著水平( P<0.05)。在施氮量為4.5 kg/hm2處理條件下,籽粒標記氮積纍量(9.76 mg/plant)分彆較莖(2.46 mg/plant)、葉(1.28 mg/plant)、莢皮(1.26 mg/plant)、葉柄(0.9 mg/plant)及根(0.41 mg/plant)高2.96、6.63、6.75、9.84和22.8倍。不同施氮處理下,各器官標記氮積纍量隨著施氮量的增加呈先增加後降低的趨勢,在施氮量為4.5 kg/hm2處理條件下達到最高值,其中籽粒中標記氮達到9.76 mg/plant。標記氮在各器官的分配比例與積纍量無明顯相關性,可能是不同施氮量下各器官榦物質積纍量不同所緻,總體錶現為籽粒>莖>葉>莢皮>葉柄>根,在施氮量為5.0 kg/hm2條件下籽粒標記氮分配率最高,為63.81%。【結論】在葉麵噴施氮4.5 kg/hm2條件下,籽粒標記氮積纍量和榦物重最高,分彆為每株9.76 mg和21.7 g。就“閤豐55”品種而言,葉麵施氮量為4.5 kg/hm2最有利于籽粒氮素及榦物質積纍。大豆鼓粒期( R5)進行葉麵施氮時,氮素主要積纍于籽粒中,有利于籽粒榦物質積纍,最終穫得增產。
【목적】협면분시담비재대두생산중이보편응용,대량연구보도표명협면분시담비능구사대두획득불동정도적증산。본연구재전인연구적기출상,채용15 N시종기술,탐색불동시담량하담소경대두협면흡수후재대두식주각조직기관적적루여분배정황,위대두협면담비적고효이용제공이론의거。【방법】재흑룡강성대두우세산구삼강평원,이해지구5년내추엄충식면적최대적대두주재품충“합봉55”위시험재료,채용15 N시종기술,이상해화공연구원생산적봉도위20.17%적15 N표기뇨소수용액위협면비료,설치불동협면담소분시량처리N 0、3.5、4.0、4.5、5.0 kg/hm2(N0、N1、N2、N3、N4),재대두중요적수담시기고립기(R5)진행협면시담처리。분석불동협면담소분시량대대두표기담흡수、분배이용규률이급대산량적영향。【결과】협면분시N 4.5 kg/hm2( N3)대두각기관간물질적루량、담소함량급담소적루량균현저고우기타처리(P<0.05)。여불시담처리(N0)상비,자립간중(21.7 g/plant)화총간물중(70.1 g/plant)분별증가6.37%화8.51%,자립담소함량(6.15 g/kg)증가10.81%,자립담소적루량(133.3 mg/plant)증가18.07%。재동일시담수평하,대두불동기관표기N적루량위자립>경>협>협피>협병>근,차이체도현저수평( P<0.05)。재시담량위4.5 kg/hm2처리조건하,자립표기담적루량(9.76 mg/plant)분별교경(2.46 mg/plant)、협(1.28 mg/plant)、협피(1.26 mg/plant)、협병(0.9 mg/plant)급근(0.41 mg/plant)고2.96、6.63、6.75、9.84화22.8배。불동시담처리하,각기관표기담적루량수착시담량적증가정선증가후강저적추세,재시담량위4.5 kg/hm2처리조건하체도최고치,기중자립중표기담체도9.76 mg/plant。표기담재각기관적분배비례여적루량무명현상관성,가능시불동시담량하각기관간물질적루량불동소치,총체표현위자립>경>협>협피>협병>근,재시담량위5.0 kg/hm2조건하자립표기담분배솔최고,위63.81%。【결론】재협면분시담4.5 kg/hm2조건하,자립표기담적루량화간물중최고,분별위매주9.76 mg화21.7 g。취“합봉55”품충이언,협면시담량위4.5 kg/hm2최유리우자립담소급간물질적루。대두고립기( R5)진행협면시담시,담소주요적루우자립중,유리우자립간물질적루,최종획득증산。
[Objectives] Forlia nitrogen application has been reported to increase soybean yield in different degrees. 15 N tracer technique was used to explore the nitrogen accumulation and distribution in different soybean organs with different nitrogen application rate, which can supply theoretical foundation for soybean leaf nitrogen efficient utilization and practical application. [Methods]This research was carried out in the soybean advantage producing area of Sanjiang Plain of Heilongjiang Province, the widely cultivated cultivar ‘HeFeng55’ in the past five years was selected as test materials. The 15N tracer technique and the 15N urea water solution(20. 17%)were produced in Shanghai Chemical Research Institute. The nitrogen spray amounts were set as:0, 3. 5, 4. 0, 4. 5 and 5. 0 kg/hm2 , recordered as N0, N1, N2, N3 and N4. Nitrogen was sprayed at the important needful nitrogen R5 stage, and the 15 N absorption and distribution, and the yield were measured. [Results]The dry weights, nitrogen contents and accumulation in the soybean organs are significantly higher with N 4. 5 kg/hm2 treatment than with other treatments(P<0. 05). The seed dry weightd and total plant dry weight are 70. 1 and 21. 7 g in treatment of N 4. 5 kg/ hm2 , with a significant increase of 8. 51% and 6. 15% compared with N0; the seeds nitrogen content is 6. 15 g/kg with an increase of 10. 81%; the seeds nitrogen accumulation is 133. 3 mg/plant with an increase of 18. 07%. Under the same nitrogen application level, the 15 N accumulation in different organs is in order of seed >stem> leaf > pod > petiole > root, and the differences reach significance level ( P<0. 05 ) . The seed nitrogen accumulation in N 4. 5 kg/hm2 is 9. 76 mg/plant, those in the stems, leaves, pods, petioles and roots are 2. 46, 1. 28, 1. 26, 0. 9 and 0. 41 mg/plant. The N accumulation in seeds is 2. 96, 6. 63, 6. 75, 9. 84 and 22. 8 times higher than the above organs. Within the tested nitrogen levels, the 15 N accumulation is first increased then decreased with the increase of nitrogen levels, and the highest seed 15 N accumulation(9. 76 mg/plant) is achived in the N 4. 5 kg/hm2 treatment. The 15 N distribution ratios and accumulation in different organs are not related to their dry biomass. The distribution ratios in different organs are all in the order of seed >stem>leaf>pod> petiole>root, and the highest seed 15 N distribution ratio(63. 81%) is in the treatment of N 5. 0 kg/hm2 . [Conclusion]The highest seed 15 N accumulation and dry weights are achieved when the forlia application rate of N is 4. 5 kg/hm2 , which should be thought the most optimum amount for the cultivar ‘HeFeng55 ’ . The appropriate forlia spray time is at the R5 stage, during which period, more applied N will be accumulated in seeds and in favour of the seed dry weight accumulation, achieving higher yield at last.