临床肺科杂志
臨床肺科雜誌
림상폐과잡지
Journal of Clinical Pulmonary Medicine
2015年
11期
2086-2089,2093
,共5页
亚甲基四氢叶酸还原酶%基因多态性%肺血栓栓塞症
亞甲基四氫葉痠還原酶%基因多態性%肺血栓栓塞癥
아갑기사경협산환원매%기인다태성%폐혈전전새증
methylene tetrahydrofolate reductase%polymorphism%pulmonary thromboembolism
目的：探讨亚甲基四氢叶酸还原酶( MTHFR)基因多态性与肺栓塞的关系。方法选取肺栓塞患者102例及同期住院或门诊患者及健康体检者120例,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法进行检测两组MTHFR基因C677T、A1298C位点的多态性,比较两组基因型和等位基因分布频率。结果①在肺栓塞组中,MTHFR C677T 位点CC、C/T、TT基因型频率分别为20．6%、31．4%、48%,在对照组中分别为29．2%、39．1%、31．7%,在两组中TT基因型频率差异有统计学意义( P=0．013)。在两组间,T等位基因分布频率差异有统计学意义(P=0．008)。②在肺栓塞组中,MTHFR A1298C位点AA、A/C、CC基因型频率分别为23．5%、31．4%、45．1%,在对照组中分别为31．7%、37．5%、30．8%,在两组中CC基因型频率差异有统计学意义(P=0．029),等位基因C分布频率两组间差异有统计学意义(P=0．018)。结论MTHFR基因C677T位点的TT基因型及A1298C位点的CC基因型多态性可能为肺血栓栓塞症的高危因素。
目的：探討亞甲基四氫葉痠還原酶( MTHFR)基因多態性與肺栓塞的關繫。方法選取肺栓塞患者102例及同期住院或門診患者及健康體檢者120例,應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法進行檢測兩組MTHFR基因C677T、A1298C位點的多態性,比較兩組基因型和等位基因分佈頻率。結果①在肺栓塞組中,MTHFR C677T 位點CC、C/T、TT基因型頻率分彆為20．6%、31．4%、48%,在對照組中分彆為29．2%、39．1%、31．7%,在兩組中TT基因型頻率差異有統計學意義( P=0．013)。在兩組間,T等位基因分佈頻率差異有統計學意義(P=0．008)。②在肺栓塞組中,MTHFR A1298C位點AA、A/C、CC基因型頻率分彆為23．5%、31．4%、45．1%,在對照組中分彆為31．7%、37．5%、30．8%,在兩組中CC基因型頻率差異有統計學意義(P=0．029),等位基因C分佈頻率兩組間差異有統計學意義(P=0．018)。結論MTHFR基因C677T位點的TT基因型及A1298C位點的CC基因型多態性可能為肺血栓栓塞癥的高危因素。
목적：탐토아갑기사경협산환원매( MTHFR)기인다태성여폐전새적관계。방법선취폐전새환자102례급동기주원혹문진환자급건강체검자120례,응용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법진행검측량조MTHFR기인C677T、A1298C위점적다태성,비교량조기인형화등위기인분포빈솔。결과①재폐전새조중,MTHFR C677T 위점CC、C/T、TT기인형빈솔분별위20．6%、31．4%、48%,재대조조중분별위29．2%、39．1%、31．7%,재량조중TT기인형빈솔차이유통계학의의( P=0．013)。재량조간,T등위기인분포빈솔차이유통계학의의(P=0．008)。②재폐전새조중,MTHFR A1298C위점AA、A/C、CC기인형빈솔분별위23．5%、31．4%、45．1%,재대조조중분별위31．7%、37．5%、30．8%,재량조중CC기인형빈솔차이유통계학의의(P=0．029),등위기인C분포빈솔량조간차이유통계학의의(P=0．018)。결론MTHFR기인C677T위점적TT기인형급A1298C위점적CC기인형다태성가능위폐혈전전새증적고위인소。
Objective To investigate the correlation between genetic polymorphism of ( Methylene tetra-hydrofolate reductase ) MTHFR and pulmonary thromboembolism. Methods The polymorphism of C677T and A1298C site on MTHFR gene in 62 patients and 70 healthy controls was detected by PCR-RFLP. Genotype and allel-ic frequencies were compared between the two groups. Results 1. The frequency of CC, CT and TT on MTHFR C677T was 20. 6%, 31. 4% and 48% in the PTE group, and 29. 2%, 39. 1% and 31. 4% in the control group, re-spectively. There was a significant difference in TT genotype frequency between the two groups ( P=0. 013 ) . The differences in allele frequencies of T were also significant between the two groups (P=0. 008). 2. The frequency of AA, A/C and CC on MTHFR A1298C was 23. 5%, 31. 4% and 45. 1% in the PTE group, and 31. 7%, 37. 5%and 30. 8% in the control group, respectively. There was a significant difference in CC genotype frequency between the two groups (P=0. 029). The differences in allele frequencies of C were significant between the two groups (P=0. 018). Conclusion The frequency of MTHFR 677TT and 1298CC genotype may be a genetic risk factor for PTE.
