CAJ | 학술논문

背景：前急进高海拔地区胃肠道急性应激损伤的模型建立鲜有报道，建立稳定的急进高海拔肠应激性损伤模型成为当前研究急进高海拔应激性胃肠损伤及相关疾病的前提。目的：建立急进高海拔引起的急性应激性肠缺血再灌注大鼠模型。方法：将雄性Wistar大鼠24只按随机数字表法分为3组，高海拔下限正常对照组、高海拔假手术组和高海拔缺血再灌注组各8只。大鼠急进海拔地区，缺血再灌注组以血管夹夹闭肠系膜上动脉根部完全阻断血流60 min，之后松开动脉血管夹，恢复肠道血流形成再灌注，时间为60 min。假手术组仅分离肠系膜上动脉不作夹闭。正常对照组不作任何处理。大鼠再灌注60 min后，以化学发光法检测肌酸激酶同工酶、谷草转氨酶、谷丙转氨酶、尿素氮、肌酐水平，并行病理组织学观察分析。结果与结论：急进高海拔并行肠缺血再灌注对大鼠肝、肾、心功能均有不同程度的损伤，肠黏膜出血，腺体断裂甚至发生溶解，黏膜层和黏膜固有层有大量炎性细胞浸润。提示采用急进高海拔地区后动脉夹夹闭肠系膜上动脉的方法制备急进高海拔地区急性应激性胃肠道损伤的大鼠模型病情相对稳定，方法简单，适于进行急进高海拔地区急性应激性胃肠道损伤的防治研究。
배경：전급진고해발지구위장도급성응격손상적모형건립선유보도，건립은정적급진고해발장응격성손상모형성위당전연구급진고해발응격성위장손상급상관질병적전제。목적：건립급진고해발인기적급성응격성장결혈재관주대서모형。방법：장웅성Wistar대서24지안수궤수자표법분위3조，고해발하한정상대조조、고해발가수술조화고해발결혈재관주조각8지。대서급진해발지구，결혈재관주조이혈관협협폐장계막상동맥근부완전조단혈류60 min，지후송개동맥혈관협，회복장도혈류형성재관주，시간위60 min。가수술조부분리장계막상동맥불작협폐。정상대조조불작임하처리。대서재관주60 min후，이화학발광법검측기산격매동공매、곡초전안매、곡병전안매、뇨소담、기항수평，병행병리조직학관찰분석。결과여결론：급진고해발병행장결혈재관주대대서간、신、심공능균유불동정도적손상，장점막출혈，선체단렬심지발생용해，점막층화점막고유층유대량염성세포침윤。제시채용급진고해발지구후동맥협협폐장계막상동맥적방법제비급진고해발지구급성응격성위장도손상적대서모형병정상대은정，방법간단，괄우진행급진고해발지구급성응격성위장도손상적방치연구。
BACKGROUND:At present, there are few reports about construction of the model of acute high altitude stress-induced gastrointestinal injury. Construction of a stable high altitude stress-induced gastrointestinal injury model has become a premise of the current studies on acute stress-induced gastrointestinal injury and related diseases. OBJECTIVE: To establish rat models of acute high altitude stress-induced gastrointestinal ischemia/reperfusion injury. METHODS:Twenty-four male Wistar rats were randomly divided into normal control (lower limit of high altitude) , sham (high altitude) and ischemia/reperfusion (high altitude) groups (n=8 rats/group). Rats from al groups underwent a rush for high altitude region. The root of the superior mesenteric artery of rats in the ischemia/reperfusion group was clamped by a vascular clip to completely block blood flow for 60 minutes, and then intestinal blood flow was recovered for reperfusion for 60 minutes. The rats in the sham group were only&nbsp