CAJ | 학술논문

目的：观察不同血清浓度对心肌细胞自噬的影响。方法以大鼠胚胎心肌细胞系H9c2为标本，建立3个时间点6、12、24 h ，以及四个不同浓度0、2、5、10％来检测细胞的自噬程度。运用Western Blot检测心肌细胞LC3Ⅱ、P62的变化，吖啶橙染色检测酸性膜泡。结果培养6 h之前，较高浓度10％血清自噬程度最低，6～12 h随着血清浓度增高自噬被促进。24 h后去血清组促进自噬，添加2，5，10％血清均能抑制自噬。结论在筛选促进自噬的因子时，应选择背景自噬较低的模型，作用效果在6 h之内的因子首选10％血清培养的心肌细胞，作用效果在6～12 h之间的选择无血清培养体系，作用效果在24 h之后的可选择有血清培养体系。
목적：관찰불동혈청농도대심기세포자서적영향。방법이대서배태심기세포계H9c2위표본，건립3개시간점6、12、24 h ，이급사개불동농도0、2、5、10％래검측세포적자서정도。운용Western Blot검측심기세포LC3Ⅱ、P62적변화，아정등염색검측산성막포。결과배양6 h지전，교고농도10％혈청자서정도최저，6～12 h수착혈청농도증고자서피촉진。24 h후거혈청조촉진자서，첨가2，5，10％혈청균능억제자서。결론재사선촉진자서적인자시，응선택배경자서교저적모형，작용효과재6 h지내적인자수선10％혈청배양적심기세포，작용효과재6～12 h지간적선택무혈청배양체계，작용효과재24 h지후적가선택유혈청배양체계。
Objective To investigate the effect of different serum concentrations on cardiomyocytes'autophagy .Methods Rat embryonic cardiac cell line H9c2 was taken as specimens to detect the levels of cellular autophagy in three time points of 6 ,12 , 24 h and four different concentrations of 0 ,2 ,5 ,10% .Western Blot was used to detect the changes of cardiac cell LC 3 Ⅱand P62 ,and acridine orange staining was used to detect acidic vesicles .Results Before 6 h ,10% concentrated serum had the low‐est level of autophagy .During 6~12 hours ,autophagy was promoted with the increasing of serum concentrations .After 24 h , serum‐deprived group promoted autophagy .Adding 2 ,5 ,10% serum could inhibit autophagy .Conclusion To find out the fac‐tors of promoting autophagy ,the models with lower autophagy should be selected .Within 6 hours ,10% concentrated serum cultured cardiac cells are the first choice .During 6~12 hours ,serum‐deprived culture system should be used .After 24 hours , serum culture system can be used .