CAJ | 학술논문

【目的】使用两个番茄品种YSL189高镉积累和HZ903低Cd积累进行苗期水培实验,旨在揭示番茄品种间Cd耐性差异的机理,为分子育种改良番茄重金属耐性提供新的信息。【方法】本研究以前期鉴定的番茄高Cd积累品种YSL189和低Cd积累品种HZ903为材料,采用苗期人工气候箱水培的方式,分析了在Cd 50和100μmol/L胁迫下,供试品种间形态学指标、抗氧化酶活性、Cd积累量及相关基因表达的差异,同时分析了供试品种苗期对离子态Cd的吸收速率差异。【结果】1)在Cd 50和100μmol/L胁迫下,根长对Cd胁迫最敏感,株高和生物量次之；与低Cd积累品种HZ903相比,高Cd积累品种YSL189表现出生物量大、根系短、株高较高的特点。2)在50和100μmol/L Cd胁迫下,植株Cd积累量以根>茎>叶的趋势递减；同等胁迫下, YSL189植株根、茎、叶部的Cd积累量都显著高于HZ903。3)当培养介质中Cd浓度大于20μmol/L时, YSL189根系对离子态Cd的吸收速率显著高于HZ903；定量PCR结果显示,在50和100μmol/L Cd胁迫下,品种间Nramp2、Nramp3和ZIP的表达量差异趋势与Cd积累的差异趋势一致,即YSL189根系Cd吸收相关基因Nramp2, Nramp3和 ZIP的表达量高于HZ903。4)在Cd 50和100μmol/L胁迫下,根、茎部的SOD(超氧化物歧化酶)、POD(过氧化物酶)和CAT(过氧化氢酶)活性都是YSL189高于HZ903,同时YSL189叶部POD和SOD活性也显著高于HZ903,同时, TBARS(巴比妥酸反应底物)含量所指示的根、茎部膜质过氧化伤害程度则没有品种间差异。【结论】YSL189根系对离子态Cd的高吸收速率以及Nramp2、Nramp3和ZIP基因的高表达量,可能是其获得高镉积累量的原因之一；同等胁迫条件下, YSL189植株体内高Cd浓度诱导了抗氧化酶的高活性,这说明YSL189植株对活性氧的清除能力强于HZ903。
【목적】사용량개번가품충YSL189고력적루화HZ903저Cd적루진행묘기수배실험,지재게시번가품충간Cd내성차이적궤리,위분자육충개량번가중금속내성제공신적신식。【방법】본연구이전기감정적번가고Cd적루품충YSL189화저Cd적루품충HZ903위재료,채용묘기인공기후상수배적방식,분석료재Cd 50화100μmol/L협박하,공시품충간형태학지표、항양화매활성、Cd적루량급상관기인표체적차이,동시분석료공시품충묘기대리자태Cd적흡수속솔차이。【결과】1)재Cd 50화100μmol/L협박하,근장대Cd협박최민감,주고화생물량차지；여저Cd적루품충HZ903상비,고Cd적루품충YSL189표현출생물량대、근계단、주고교고적특점。2)재50화100μmol/L Cd협박하,식주Cd적루량이근>경>협적추세체감；동등협박하, YSL189식주근、경、협부적Cd적루량도현저고우HZ903。3)당배양개질중Cd농도대우20μmol/L시, YSL189근계대리자태Cd적흡수속솔현저고우HZ903；정량PCR결과현시,재50화100μmol/L Cd협박하,품충간Nramp2、Nramp3화ZIP적표체량차이추세여Cd적루적차이추세일치,즉YSL189근계Cd흡수상관기인Nramp2, Nramp3화 ZIP적표체량고우HZ903。4)재Cd 50화100μmol/L협박하,근、경부적SOD(초양화물기화매)、POD(과양화물매)화CAT(과양화경매)활성도시YSL189고우HZ903,동시YSL189협부POD화SOD활성야현저고우HZ903,동시, TBARS(파비타산반응저물)함량소지시적근、경부막질과양화상해정도칙몰유품충간차이。【결론】YSL189근계대리자태Cd적고흡수속솔이급Nramp2、Nramp3화ZIP기인적고표체량,가능시기획득고력적루량적원인지일；동등협박조건하, YSL189식주체내고Cd농도유도료항양화매적고활성,저설명YSL189식주대활성양적청제능력강우HZ903。
[Objectives]To investigate the different responses of Cd accumulation in tomato cultivars exposed to Cadmium stress, a hydroponics experiment at seedling stage was performed using two tomato varieties, YSL189 with high Cd accumulation and HZ903 with low Cd accumulation, which were confirmed by our previous research.[Methods]The experiment was performed in growth chamber, and morphological index, antioxidant enzyme activity, differences in Cd accumulation, ionic absorption rate and related gene expression in varieties were analyzed using seedling exposed to Cd 50 and 100 μmol/L. [Results] When exposed to 50 and 100 μmol/L of Cd, the root length of plants was more sensitive to Cd stress than plant height and biomass. Cultivar YSL189 exhibited higher tolerance in biomass and plant height, but lower root length than HZ903. Under both 50 and 100μmol/L Cd treatments, the accumulation of Cd was reduced significantly in the order of roots, stem and leaves accordingly, and the Cd concentration in roots, stem and leaves of YSL189 was significantly higher than in those of HZ903. When the concentration of Cd in culture medium was over 20μmol/L, the uptake rate of Cd in the roots of YSL189 was significantly higher than that in the roots of HZ903. The results of Real-Time PCR proved that theexpression levels of transporter genes Nramp2 , Nramp3 and ZIP in the roots of YSL 189 were higher than those in the roots of HZ903, and higher activities of SOD(superoxide dismutase), POD(peroxidase) and CAT(atalase) in roots and stems, SOD and POD in leaves were higher in YSL189 than those in HZ903, but no significant difference in concentration of TBARS ( thiobarbituric acid reactive substance ) between cultivars was found, indicating the degree of lipid peroxidation. [Conclusion] The higher Cd accumulation in YSL189 may be partly attributed to the higher Cd uptake rate, expression levels of Nramp2, Nramp3, and ZIP in roots of YSL189 were higher than those of HZ903;and the higher activities of antioxidant enzymes in YSL189 were induced by higher Cd concentration, indicating the stronger power to detoxify reactive oxygen species for YSL189 than HZ903 .