CAJ | 학술논문

目的：了解不同分期喉癌患者手术前后的肿瘤-睾丸抗原（CTA）家族中的黑色素瘤抗原-A1（MAGE-A1）和黑色素瘤抗原-A3（MAGE-A3）的血清学浓度变化情况，筛选喉癌诊断的CTA血清标志物。方法采用酶联免疫吸附试验（ELISA）法检测25例喉癌患者手术前、手术后血清中的MAGE-A1和MAGE-A3水平，并进一步检测46例喉癌患者手术前组、33例喉癌患者手术后组和20名健康人血清中的5种CTA。结果 ELISA法检测25例喉癌患者手术前、手术后血清中MAGE-A1和MAGE-A3浓度差异有统计学意义（P＜0.05）。在46例喉癌患者手术前组、33例喉癌患者手术后组和20名健康人中，MAGE-A1和MAGE-A3手术前组和健康人组血清的浓度差异有统计学意义（P＜0.05）。结论 MAGE-A1和MAGE-A3有可能成为喉癌诊断和预测复发的CTA血清标志物。
목적：료해불동분기후암환자수술전후적종류-고환항원（CTA）가족중적흑색소류항원-A1（MAGE-A1）화흑색소류항원-A3（MAGE-A3）적혈청학농도변화정황，사선후암진단적CTA혈청표지물。방법채용매련면역흡부시험（ELISA）법검측25례후암환자수술전、수술후혈청중적MAGE-A1화MAGE-A3수평，병진일보검측46례후암환자수술전조、33례후암환자수술후조화20명건강인혈청중적5충CTA。결과 ELISA법검측25례후암환자수술전、수술후혈청중MAGE-A1화MAGE-A3농도차이유통계학의의（P＜0.05）。재46례후암환자수술전조、33례후암환자수술후조화20명건강인중，MAGE-A1화MAGE-A3수술전조화건강인조혈청적농도차이유통계학의의（P＜0.05）。결론 MAGE-A1화MAGE-A3유가능성위후암진단화예측복발적CTA혈청표지물。
Objective To investigate the serological concentration changes of melanoma antigen-A1 (MAGE-A1) and melanoma antigen-A3 (MAGE-A3) of the cancer-testis antigen (CTA) family in patients with laryngeal carci-noma at different stages before and after the operation, and to screen the CTA serum markers of the laryngeal carcino-ma diagnosis. Methods Enzyme-linked immunosorbent assay (ELISA) was used to determine the serum MAGE-A1 and MAGE-A3 in 25 patients with laryngeal cell carcinoma (LCC) before and after the operation. Then, 5 CTA serum markers were determined in the preoperative LCC group (n=46), postoperative LCC group (n=33) and normal group (n=20). Results ELISA showed that there were significant differences between preoperative and postoperative serum MAGE-A1 and MAGE-A3 concentration in 25 LCC patients (P<0.05). Among the preoperative LCC group (n=46), postoperative LCC group (n=33) and normal group (n=20), there were significant differences in serum MAGE-A1 and MAGE-A3 concentration between the preoperative groups and normal group (P<0.05). Conclusion MAGE-A1 and MAGE-A3 may become CTA serum markers for the LCC diagnosis and prediction of LSCC recurrence.