中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
Chinese Journal of Biochemical Pharmaceutics
2015年
9期
19-22
,共4页
贾越光%丁志英%肖嘉婧%张新然%池季洪%孙波
賈越光%丁誌英%肖嘉婧%張新然%池季洪%孫波
가월광%정지영%초가청%장신연%지계홍%손파
人参皂苷%纳米乳%抗衰老%美白%安全性
人參皂苷%納米乳%抗衰老%美白%安全性
인삼조감%납미유%항쇠로%미백%안전성
ginsenoside%nano emulsion%anti-aging%whitening%security
目的：研究人参皂苷纳米乳的美白抗衰作用及其安全性。方法美白作用：用维生素C为对照药品，测定酪氨酸酶抑制率。抗衰老作用：建立D-半乳糖大鼠衰老模型，在大鼠颈背部皮肤上涂抹人参皂苷纳米乳及对照药品，每日2次，制备涂片，比较皮肤组织纤维的排列变化，测定DPPH自由基清除率。安全性评价：观察皮肤涂抹人参皂苷纳米乳及对照药品后，不同时间的皮肤变化情况。结果人参皂苷浓度为7.00 mg／mL时，酪氨酸酶抑制率顺序是，维生素C水溶液＞人参皂苷纳米乳＞人参皂苷水溶液；在衰老大鼠皮肤上涂抹人参皂苷纳米乳，切片结果显示，弹力纤维与皮肤表面较为平行，而未涂抹的对照组，弹力纤维排列较为曲折，与皮肤表面不平行；DPPH自由基清除率结果显示，维生素C水溶液＞人参皂苷纳米乳＞人参皂苷水溶液，且随着人参皂苷载药量的增大，人参皂苷纳米乳清除DPPH自由基的效率明显上升；安全性结果显示，皮肤涂抹人参皂苷纳米乳后，没有红肿、刺激等现象发生。结论人参皂苷纳米乳比人参皂苷水溶液具有更明显的美白抗衰作用，没有刺激，不会对皮肤造成损伤，安全可靠。
目的：研究人參皂苷納米乳的美白抗衰作用及其安全性。方法美白作用：用維生素C為對照藥品，測定酪氨痠酶抑製率。抗衰老作用：建立D-半乳糖大鼠衰老模型，在大鼠頸揹部皮膚上塗抹人參皂苷納米乳及對照藥品，每日2次，製備塗片，比較皮膚組織纖維的排列變化，測定DPPH自由基清除率。安全性評價：觀察皮膚塗抹人參皂苷納米乳及對照藥品後，不同時間的皮膚變化情況。結果人參皂苷濃度為7.00 mg／mL時，酪氨痠酶抑製率順序是，維生素C水溶液＞人參皂苷納米乳＞人參皂苷水溶液；在衰老大鼠皮膚上塗抹人參皂苷納米乳，切片結果顯示，彈力纖維與皮膚錶麵較為平行，而未塗抹的對照組，彈力纖維排列較為麯摺，與皮膚錶麵不平行；DPPH自由基清除率結果顯示，維生素C水溶液＞人參皂苷納米乳＞人參皂苷水溶液，且隨著人參皂苷載藥量的增大，人參皂苷納米乳清除DPPH自由基的效率明顯上升；安全性結果顯示，皮膚塗抹人參皂苷納米乳後，沒有紅腫、刺激等現象髮生。結論人參皂苷納米乳比人參皂苷水溶液具有更明顯的美白抗衰作用，沒有刺激，不會對皮膚造成損傷，安全可靠。
목적：연구인삼조감납미유적미백항쇠작용급기안전성。방법미백작용：용유생소C위대조약품，측정락안산매억제솔。항쇠로작용：건립D-반유당대서쇠로모형，재대서경배부피부상도말인삼조감납미유급대조약품，매일2차，제비도편，비교피부조직섬유적배렬변화，측정DPPH자유기청제솔。안전성평개：관찰피부도말인삼조감납미유급대조약품후，불동시간적피부변화정황。결과인삼조감농도위7.00 mg／mL시，락안산매억제솔순서시，유생소C수용액＞인삼조감납미유＞인삼조감수용액；재쇠로대서피부상도말인삼조감납미유，절편결과현시，탄력섬유여피부표면교위평행，이미도말적대조조，탄력섬유배렬교위곡절，여피부표면불평행；DPPH자유기청제솔결과현시，유생소C수용액＞인삼조감납미유＞인삼조감수용액，차수착인삼조감재약량적증대，인삼조감납미유청제DPPH자유기적효솔명현상승；안전성결과현시，피부도말인삼조감납미유후，몰유홍종、자격등현상발생。결론인삼조감납미유비인삼조감수용액구유경명현적미백항쇠작용，몰유자격，불회대피부조성손상，안전가고。
Objective To study on whitening and anti -aging effect of ginseng saponin and its safety.Methods Whitening effect:using vitamin C as the control drug, the inhibition rate of tyrosinase was determined.Anti senescence effect: the aging model of D-was established, and the DPPH was applied to the skin of the rat's neck, and the drug was prepared by 2 times a day.Skin safety evaluation: the skin changes of the skin of the ginseng saponins were observed after the skin was given ginseng saponin and control drugs.Results When the concentration of ginsenoside was 7 mg/mL, the inhibition rate order was, the water solution of vitamin C >ginsenoside nano >ginsenoside aqueous solution ; In the aging model, the surface of the elastic fibers and the skin surface was parallel to the skin, but the elastic fibers were arranged in a more tortuous and non parallel to the skin;DPPH free radical scavenging effect order was, Vitamin C aqueous solution>ginsenoside nano>ginsenoside aqueous solution, and with the dosage of ginsenoside increasing, the efficiency of ginsenoside DPPH scavenging free radical increased significantly;Skin safety evaluation results showed that ginseng saponin nanometer milk smeared skin, no redness, irritation and other phenomena occured.Conclusion Ginseng saponin nanometer milk has obvious whitening effect, and can not cause damage to the skin, is safe and reliable.
