中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
Chinese Journal of Biochemical Pharmaceutics
2015年
9期
15-18
,共4页
陈仕学%姚元勇%卢忠英%唐帮成
陳仕學%姚元勇%盧忠英%唐幫成
진사학%요원용%로충영%당방성
吴茱萸%阳离子交换树脂%纯化工艺%总碱
吳茱萸%暘離子交換樹脂%純化工藝%總堿
오수유%양리자교환수지%순화공예%총감
Evodia rutaecarpa%cation exchange resin%extraction process%total alkaloids
目的：研究吴茱萸总碱最佳纯化工艺路线及其抗乳腺癌活性。方法分别采用传统提取纯化和732型阳离子交换树脂法提取纯化吴茱萸总碱，用碘化汞钾试剂对其进行定性分析，并对洗脱剂进行选择。应用MTT实验对所提取的吴茱萸总碱进行抗乳腺癌活性测试。结果与传统提取工艺相比，732型阳离子交换树脂法提取吴茱萸总碱的产率较高（16.18％）；732型阳离子树脂纯化法提取吴茱萸总碱的最佳洗脱剂为饱和氯化钠溶液；细胞毒性实验结果表明U251乳腺癌细胞对吴茱萸总碱的浓度依赖性不强，即随着载药浓度的增加，细胞的形态改变不大、凋亡的细胞数量增多不明显。当浓度为50μmol／mL，作用时间为24 h时，细胞的存活率为68％；随着浓度的增加（100、150μmol／mL），作用时间为24 h时，细胞的存活率分别为66％和60％。结论与传统提取法相比，732型阳离子树脂纯化法提取的吴茱萸总碱纯度较高；同时吴茱萸总碱对乳腺癌细胞具有明显的抑制作用。
目的：研究吳茱萸總堿最佳純化工藝路線及其抗乳腺癌活性。方法分彆採用傳統提取純化和732型暘離子交換樹脂法提取純化吳茱萸總堿，用碘化汞鉀試劑對其進行定性分析，併對洗脫劑進行選擇。應用MTT實驗對所提取的吳茱萸總堿進行抗乳腺癌活性測試。結果與傳統提取工藝相比，732型暘離子交換樹脂法提取吳茱萸總堿的產率較高（16.18％）；732型暘離子樹脂純化法提取吳茱萸總堿的最佳洗脫劑為飽和氯化鈉溶液；細胞毒性實驗結果錶明U251乳腺癌細胞對吳茱萸總堿的濃度依賴性不彊，即隨著載藥濃度的增加，細胞的形態改變不大、凋亡的細胞數量增多不明顯。噹濃度為50μmol／mL，作用時間為24 h時，細胞的存活率為68％；隨著濃度的增加（100、150μmol／mL），作用時間為24 h時，細胞的存活率分彆為66％和60％。結論與傳統提取法相比，732型暘離子樹脂純化法提取的吳茱萸總堿純度較高；同時吳茱萸總堿對乳腺癌細胞具有明顯的抑製作用。
목적：연구오수유총감최가순화공예로선급기항유선암활성。방법분별채용전통제취순화화732형양리자교환수지법제취순화오수유총감，용전화홍갑시제대기진행정성분석，병대세탈제진행선택。응용MTT실험대소제취적오수유총감진행항유선암활성측시。결과여전통제취공예상비，732형양리자교환수지법제취오수유총감적산솔교고（16.18％）；732형양리자수지순화법제취오수유총감적최가세탈제위포화록화납용액；세포독성실험결과표명U251유선암세포대오수유총감적농도의뢰성불강，즉수착재약농도적증가，세포적형태개변불대、조망적세포수량증다불명현。당농도위50μmol／mL，작용시간위24 h시，세포적존활솔위68％；수착농도적증가（100、150μmol／mL），작용시간위24 h시，세포적존활솔분별위66％화60％。결론여전통제취법상비，732형양리자수지순화법제취적오수유총감순도교고；동시오수유총감대유선암세포구유명현적억제작용。
Objective To optimize conditions for extraction of alkaloids from Evodia rutaecarpa by means of 732 cation exchange resin and performe its anti-breast cancer bioactivity.Methods The optimum processing route on extraction of alkaloids from Evodia rutaecarpa was investigated by means of 732 cation exchange resin.The performance of 732 cation exchange resin was compared with tranditonal process ( aqueous extraction-ethanol precipitation in extraction-purification process).The alkaloid reagent-potassium mercuric iodide test and MTT test were performed on the crudes.Results Compared with traditional purification process, it was much better to use 732 cation exchange resin approach for extraction of alkaloids from Evodia rutaecarp with high yield(16.81%) The best eluent should be saturated brine with excellent purification.No obvious correlations were found between the toxin of breast cancer cell and concentration of total alkaloids.When the concentration of total alkaloids was 50μmol/mL, cellular survival rate was 68%afterward 24 h.When the concentration of total alkaloids comes to 100 μmol/mL and 150 μmol/mL, cellular survival rates were slightly decreased by 66% and 60%.Conclusion 732 cation exchange resin performes much higher than traditional process in purification of total alkaloids extracted from Evodia rutaecarpa.Simultaneously, the extracted total alkaloids showe remarkable inhibition in breast cancer cell.
