CAJ | 학술논문

目的：采用不同浓度丹参酮IIA磺酸钠( STS)治疗火器伤引起豚鼠ALI时的肺保护作用。方法60只豚鼠随机分为正常对照组为N组、模型组为M组、生理盐水对照组为P组、低剂量STS治疗组( A组)、中剂量STS治疗组(B组)、高剂量STS治疗组(C组)，每组10只，制作豚鼠ALI动物模型。造模0.5h后，采用免疫印迹法测定细胞核因子( NF-κB)，用光学显微镜观察实验豚鼠病理变化情况，并及时记录其观察结果。结果病理学结果除N组外，造模12h各组动物的肺泡结构破坏，结构混乱，各种炎性细胞浸润，以M组最明显；用丹参酮IIA干预治疗各组中均有改善，以中等浓度STS干预治疗为最有效( P<0．05)。结论STS治疗对火器伤引起的豚鼠ALI具有保护作用，以STS中剂量保护肺作用最明显。
목적：채용불동농도단삼동IIA광산납( STS)치료화기상인기돈서ALI시적폐보호작용。방법60지돈서수궤분위정상대조조위N조、모형조위M조、생리염수대조조위P조、저제량STS치료조( A조)、중제량STS치료조(B조)、고제량STS치료조(C조)，매조10지，제작돈서ALI동물모형。조모0.5h후，채용면역인적법측정세포핵인자( NF-κB)，용광학현미경관찰실험돈서병리변화정황，병급시기록기관찰결과。결과병이학결과제N조외，조모12h각조동물적폐포결구파배，결구혼란，각충염성세포침윤，이M조최명현；용단삼동IIA간예치료각조중균유개선，이중등농도STS간예치료위최유효( P<0．05)。결론STS치료대화기상인기적돈서ALI구유보호작용，이STS중제량보호폐작용최명현。
Objective To study the lung protective effect of tanshinone IIA sulfoacid sodium ( STS) in the treatment of firearm injury-induced acute lung injury ( ALI) . Methods 60 guinea pigs were randomly divided into six groups:the normal control group ( Group N) , the acute lung injury model group ( Group M) , the physiological saline injection group ( Group P) , the low dose of STS treatment group ( Group A) , the middle dose of STS treatment group (Group B), and the high dose of STS treatment group (Group C). All groups had 10 guinea pigs to be in-duced with ALI. 0. 5 hours after modeling, the expression of NF-κB in lung tissues was detected by Western blot, and pathological changes in guinea pigs were observed by optical microscope. Results Except the N group, guinea pigs in the rest five groups showed alveolar structure damage and disorder, and inflammatory cell infiltration, with the most obvious in the group M. All groups improved after STS intervention, and the medium concentration of STS had the most effective effect (P<0. 05). Conclusion STS has the protective effect for guinea pigs with firearm injury-induced acute lung injury, and the middle dose has the best effect.