南昌大学学报（理科版）
南昌大學學報（理科版）
남창대학학보（이과판）
Journal of Nanchang University (Natural Science)
2015年
3期
275-279
,共5页
秦燕%邓泽元%姚昭%李红艳
秦燕%鄧澤元%姚昭%李紅豔
진연%산택원%요소%리홍염
有机硒%无机硒%VE%复配%原子荧光光度法
有機硒%無機硒%VE%複配%原子熒光光度法
유궤서%무궤서%VE%복배%원자형광광도법
Organic selenium%inorganic selenium%Vitamin E%supplements containing selenium%AFS
探究大鼠摄食不同形态硒和复配后，肾脏、肝脏、心脏和骨骼中硒的沉积规律。大鼠连续灌胃有机硒（L-SeMC），无机硒（亚硒酸钠），有机硒＋VE，L-SeMC＋VE＋原花青素等30 d 后，采集其肾脏、肝脏、心脏和骨骼，经原子荧光光度法测定各组织总硒含量。结果显示：添加不同形态的硒对大鼠各组织硒沉积量均有所提高；有机硒与 VE 联用组硒含量比单独添加有机硒或无机硒组有显著性提高（P ＜0．05），表明 VE 可促进硒在组织中的沉积；L-SeMC＋VE＋原花青素组大鼠组织中的硒沉积量与硒的添加量成正相关关系，但其大鼠组织中硒沉积量低于其他同等剂量硒添加组，表明原花青素可能会降低硒在组织中的沉积量。结论：添加不同形式的硒均能提高大鼠组织硒的沉积量；有机硒与 VE 联用能明显促进组织中硒元素的沉积；在一定范围内，硒在组织中的沉积量随含硒剂量的增加而升高。
探究大鼠攝食不同形態硒和複配後，腎髒、肝髒、心髒和骨骼中硒的沉積規律。大鼠連續灌胃有機硒（L-SeMC），無機硒（亞硒痠鈉），有機硒＋VE，L-SeMC＋VE＋原花青素等30 d 後，採集其腎髒、肝髒、心髒和骨骼，經原子熒光光度法測定各組織總硒含量。結果顯示：添加不同形態的硒對大鼠各組織硒沉積量均有所提高；有機硒與 VE 聯用組硒含量比單獨添加有機硒或無機硒組有顯著性提高（P ＜0．05），錶明 VE 可促進硒在組織中的沉積；L-SeMC＋VE＋原花青素組大鼠組織中的硒沉積量與硒的添加量成正相關關繫，但其大鼠組織中硒沉積量低于其他同等劑量硒添加組，錶明原花青素可能會降低硒在組織中的沉積量。結論：添加不同形式的硒均能提高大鼠組織硒的沉積量；有機硒與 VE 聯用能明顯促進組織中硒元素的沉積；在一定範圍內，硒在組織中的沉積量隨含硒劑量的增加而升高。
탐구대서섭식불동형태서화복배후，신장、간장、심장화골격중서적침적규률。대서련속관위유궤서（L-SeMC），무궤서（아서산납），유궤서＋VE，L-SeMC＋VE＋원화청소등30 d 후，채집기신장、간장、심장화골격，경원자형광광도법측정각조직총서함량。결과현시：첨가불동형태적서대대서각조직서침적량균유소제고；유궤서여 VE 련용조서함량비단독첨가유궤서혹무궤서조유현저성제고（P ＜0．05），표명 VE 가촉진서재조직중적침적；L-SeMC＋VE＋원화청소조대서조직중적서침적량여서적첨가량성정상관관계，단기대서조직중서침적량저우기타동등제량서첨가조，표명원화청소가능회강저서재조직중적침적량。결론：첨가불동형식적서균능제고대서조직서적침적량；유궤서여 VE 련용능명현촉진조직중서원소적침적；재일정범위내，서재조직중적침적량수함서제량적증가이승고。
The current stuy aimed to investigate the deposition regularity of selenium on rat's kidney,liver, heart and bones after been fed with different forms of selenium.The rats were fed by gavage once a day for 4 weeks with organic selenium (L-SeMC),inorganic selenium (Na2SeO3),vitamin E and supplements (containing L-SeMC、Na2 SeO3 and procyanidins),respectively.The samples of kidney,liver,heart and bones from the sacrificed rats were collected and digested by a wet digestion protocol.Total selenium con-tent was determined by Atomic Fluorescence Spectrometry (AFS).In this study,we found that the deposi-tion of selenium in rats tissues increased after fed with the two forms of selenium in the experiment.More-over,when compared with organic selenium/VE group,organic group and VE group,the total selenium content in the selenium/VE group was significantly higher than the other two groups except in kidney (P<0.05),indicating that VE accelerated the deposition of selenium in rat tissues.Different doses of supple-ment groups revealed that the tissues selenium content was proportional to the dose of supplements.But the selenium deposition in supplements group was lower than the organic/inorganic group with the same dose of selenium.It could be speculated that procyanidins might inhibit the deposition of selenium in tis-sues.In conclusion,different forms of selenium exhibited good effects of selenium deposition in rat tissues. Combination of organic selenium and VE significantly increased the total selenium content.Within certain limits,the tissue selenium content increased with the dose of selenium raised.
