CAJ | 학술논문

目的：分离黄蜀葵茎枯病病原菌并鉴定其种类。方法取江苏省宜兴市采集的黄蜀葵发病植株病灶处（ J）和健康处（ W）的茎段，培养并筛选新生菌丝体，将分离获得的几种待定病原菌分别接种至健康黄蜀葵幼苗茎部，进行致病性检测，随后将获得的有效致病菌株进行病原菌形态学鉴定、PCR扩增和rDNA-ITS检测以确定菌种。结果排除J与W中一致的菌种，分离得到3种真菌J2，J5和J6；致病性检测结果发现J5为有效致病菌；根据病原菌形态特征观察，初步鉴定J5为木贼镰刀菌；J5病原菌基因组DNA扩增后得到了长度为524 bp的条带，与木贼镰刀菌（Fusarium equiseti）的ITS序列同源性最高，达到了100％。结论黄蜀葵茎枯病病原菌为木贼镰刀菌F.equiseti。
목적：분리황촉규경고병병원균병감정기충류。방법취강소성의흥시채집적황촉규발병식주병조처（ J）화건강처（ W）적경단，배양병사선신생균사체，장분리획득적궤충대정병원균분별접충지건강황촉규유묘경부，진행치병성검측，수후장획득적유효치병균주진행병원균형태학감정、PCR확증화rDNA-ITS검측이학정균충。결과배제J여W중일치적균충，분리득도3충진균J2，J5화J6；치병성검측결과발현J5위유효치병균；근거병원균형태특정관찰，초보감정J5위목적렴도균；J5병원균기인조DNA확증후득도료장도위524 bp적조대，여목적렴도균（Fusarium equiseti）적ITS서렬동원성최고，체도료100％。결론황촉규경고병병원균위목적렴도균F.equiseti。
Objective To isolation and identify the pathogen of stem blight of Malvaceae.Methods The stems were collected from stem blight-diseased plants (J) and healthy ones (W) of Abelmoschus manihot (L.) Medic.in Yixing City of Jiangsu Province then cultured to isolate newborn mycelium.The pathogen isolated but unidentified were inoculated in stems of healthy plants of Abelmoschus manihot ( L.) Medic.and pathogenicity was verified.Finally, the pathogenic specie( s) was or were identified by morphological characteristic, rDNA-ITS analysis and polymerase chain reaction (PCR) method.Results The same fungus were excluded which were the same species in J and W, the three fungus of J2, J5 and J6 were acquired.J5 was preliminarily identified to have pathogenicity and it was Fusarium equiseti under the microscope.The genome DNA of J5 was amplified to a length of 524bp, and homology highly with Fusarium equiseti (100%).Conclusion The pathogen was identified as Fusarium equiseti.