CAJ | 학술논문

免疫磁分离是一种食源性致病菌特异分离与高效富集的方法。由于常规的磁力架存在磁场强度和梯度均较低的不足，很难高效捕获纳米磁珠，因此本研究开发了一种新型的纳米磁分离器，并以大肠杆菌 O157∶ H7为研究模型，利用平板菌落计数法，从分离时间、重复性和细菌浓度范围等方面开展了该纳米磁分离器结合不同粒径的纳米磁珠对大肠杆菌的分离效率评价。实验结果表明：对于30、100和180 nm 磁珠，当磁分离时间分别大于60 min、60 s 和40 s 时，该纳米磁分离器对102－106 CFU·mL－1的大肠杆菌的捕获效率不小于95％，平行实验相对标准偏差不大于7．0％。
면역자분리시일충식원성치병균특이분리여고효부집적방법。유우상규적자력가존재자장강도화제도균교저적불족，흔난고효포획납미자주，인차본연구개발료일충신형적납미자분리기，병이대장간균 O157∶ H7위연구모형，이용평판균락계수법，종분리시간、중복성화세균농도범위등방면개전료해납미자분리기결합불동립경적납미자주대대장간균적분리효솔평개。실험결과표명：대우30、100화180 nm 자주，당자분리시간분별대우60 min、60 s 화40 s 시，해납미자분리기대102－106 CFU·mL－1적대장간균적포획효솔불소우95％，평행실험상대표준편차불대우7．0％。
Immune magnetic separation is a new method for separation and concentration of foodborne pathogens.The conventional magnetic separator with low strength and gradient could not capture magnetic beads in nano sizes.This study developed a magnetic bioseparator based nano-sized magnetic beads and it was evaluated using E.coli O157∶H7 as model and conventional standard method,colony counting,for the determination of E.coli cells.Three different sizes of magnetic nanoparticles with the diameters of 30 nm, 100 nm and 180 nm were used for separation of E.coli cells at the concentrations of 102 -10 6 CFU·mL-1 . When the magnetic separation times were no less than 60 min,60 s and 40 s respectively,the capture effi-ciency of E.coli cells was no less than 95%.The relative standard deviations for parallel tests were less than 7.0%.