CAJ | 학술논문

目的：评价支气管肺泡灌洗液结核分枝杆菌RNA恒温扩增检测( simultaneous amplification and testing for Mycobacterium tuberculosis, SAT-TB)对涂阴肺结核的诊断价值。方法选取涂阴肺结核组(观察组)95例和非结核病组(对照组)67例共计162例,两组患者均行纤维支气管镜检查,留取支气管肺泡灌洗液行荧光定量PCR( FQ-PCR),RNA恒温扩增检测( SAT-TB),结核菌快速培养,计算BALF 的SAT-TB对涂阴肺结核诊断的敏感度,特异度,阳性预测值,阴性预测值。结果 SAT-TB检测BALF对诊断涂阴肺结核的敏感度为53．7%,特异度为98．5%,阳性预测值为98．1%,阴性预测值为60．0%,BALF的SAT-TB检测在敏感度,特异度,阳性预测值,阴性预测值与FQ-PCR法相比较均无显著性差异(χ2值分别为3．572,0．341,0．679,1．603,P值均>0．05)。结论 BALF中SAT-TB检测在涂阴肺结核中的敏感度,特异度均较高,且检测快速,对涂阴肺结核的快速诊断具有较大的应用价值。
목적：평개지기관폐포관세액결핵분지간균RNA항온확증검측( simultaneous amplification and testing for Mycobacterium tuberculosis, SAT-TB)대도음폐결핵적진단개치。방법선취도음폐결핵조(관찰조)95례화비결핵병조(대조조)67례공계162례,량조환자균행섬유지기관경검사,류취지기관폐포관세액행형광정량PCR( FQ-PCR),RNA항온확증검측( SAT-TB),결핵균쾌속배양,계산BALF 적SAT-TB대도음폐결핵진단적민감도,특이도,양성예측치,음성예측치。결과 SAT-TB검측BALF대진단도음폐결핵적민감도위53．7%,특이도위98．5%,양성예측치위98．1%,음성예측치위60．0%,BALF적SAT-TB검측재민감도,특이도,양성예측치,음성예측치여FQ-PCR법상비교균무현저성차이(χ2치분별위3．572,0．341,0．679,1．603,P치균>0．05)。결론 BALF중SAT-TB검측재도음폐결핵중적민감도,특이도균교고,차검측쾌속,대도음폐결핵적쾌속진단구유교대적응용개치。
Objective To evaluate the value of simultaneous amplification and testing for mycobacterium tuberculosis ( SAT-TB) in bronchoalveolar lavage fluid ( BALF) in the diagnosis of smear-negative pulmonary tuber-culosis ( PTB) . Methods The study selected 95 cases of active pulmonary tuberculosis as the observation group and 67 cases of non-active pulmonary tuberculosis as the control group. BALF was obtained by brochchoscopy and then tested by FQ-PCR, SAT-TB assay and Bactec MGIT 960 culture. The sensitivity, specificity, PPV and NPV for each diagnostic test were calculated. Results The sensitivity, specificity, positive prediction value, negative prediction value of SAT-TB for smear-negative PTB were 53. 7%, 98. 5%, 98. 1% and 60. 0% respectively. There was no sig-nificantly difference between SAT-TB and FQ-PCR in sensitivity (χ2 =3. 572, P>0. 05), specificity (χ2 =0. 341, P>0. 05), positive prediction value (χ2 =0. 679, P>0. 05), negative prediction value (χ2 =1. 063, P>0. 05). Conclusion The sensitivity and specificity of SAT-TB in BALF are very high and the detection time of SAT-TB is very short, which has a good application value for fast diagnosis in smear-negative PTB.