中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
Chinese Journal of Biochemical Pharmaceutics
2015年
9期
8-11
,共4页
张海静%胡兆秋%报孙玉卿%冯占芹%商洪才%张维芬
張海靜%鬍兆鞦%報孫玉卿%馮佔芹%商洪纔%張維芬
장해정%호조추%보손옥경%풍점근%상홍재%장유분
放射性肺损伤%黄芪多糖%炎症细胞%肺泡灌洗液
放射性肺損傷%黃芪多糖%炎癥細胞%肺泡灌洗液
방사성폐손상%황기다당%염증세포%폐포관세액
radio-pulmonary lesion%astragaluspolysaccharides%inflammation cells%bronchoalveolar lavage fluid
目的：探讨黄芪多糖对大鼠急性放射性肺损伤的治疗作用。方法48只Wistar大鼠随机分成正常组、模型组、黄芪多糖组、地塞米松组，每组12只。后3组大鼠采用6MV-X线直线加速器对大鼠双肺进行照射（20 Gy／次）造模，随后分别给予0.9％氯化钠注射液、黄芪多糖（8 mg／mL）和地塞米松（0.05 mg／mL）持续灌胃治疗8周，正常组佯装照射，给予生理盐水灌胃于放疗后2、8周，每组随机抽取6只大鼠处死，心脏取血进行白细胞、红细胞计数；取各组大鼠右上肺组织，HE染色观察病理改变。结果成功构建了放射性肺损伤大鼠模型，一般情况观察显示：照射后的大鼠陆续出现精神萎靡，活动量减少、弓背、脱毛等症状，黄芪多糖及地塞米松处理后的大鼠与模型组比较，脱毛等现象逐渐改善。正常组未见任何明显异常变化。与正常组比较，模型组大鼠血液中白细胞及红细胞计数明显降低（P＜0.05），肺泡灌洗液（bronchoalveolar lavage fluid，BALF）中白细胞及红细胞明显升高（P＜0.05）；与模型组比较，地塞米松组与黄芪多糖组血液中白细胞及红细胞计数明显回升（ P＜0.05），且BALF中红细胞及白细胞明显降低（ P＜0.05）；但黄芪多糖组与地塞米松组比较差异无统计学意义。肺组织病理学显示：模型组较正常组大鼠，肺泡壁破裂、肺泡间隔增宽、肺间质增生、肺泡腔及肺间质有大量炎性细胞渗出；地塞米松组及黄芪多糖组大鼠均较模型组明显减轻，但2者之间差异无统计学意义。结论黄芪多糖能显著改善大鼠急性放射性肺损伤，作用与地塞米松相近，为放射性肺损伤提供了治疗新方法。
目的：探討黃芪多糖對大鼠急性放射性肺損傷的治療作用。方法48隻Wistar大鼠隨機分成正常組、模型組、黃芪多糖組、地塞米鬆組，每組12隻。後3組大鼠採用6MV-X線直線加速器對大鼠雙肺進行照射（20 Gy／次）造模，隨後分彆給予0.9％氯化鈉註射液、黃芪多糖（8 mg／mL）和地塞米鬆（0.05 mg／mL）持續灌胃治療8週，正常組佯裝照射，給予生理鹽水灌胃于放療後2、8週，每組隨機抽取6隻大鼠處死，心髒取血進行白細胞、紅細胞計數；取各組大鼠右上肺組織，HE染色觀察病理改變。結果成功構建瞭放射性肺損傷大鼠模型，一般情況觀察顯示：照射後的大鼠陸續齣現精神萎靡，活動量減少、弓揹、脫毛等癥狀，黃芪多糖及地塞米鬆處理後的大鼠與模型組比較，脫毛等現象逐漸改善。正常組未見任何明顯異常變化。與正常組比較，模型組大鼠血液中白細胞及紅細胞計數明顯降低（P＜0.05），肺泡灌洗液（bronchoalveolar lavage fluid，BALF）中白細胞及紅細胞明顯升高（P＜0.05）；與模型組比較，地塞米鬆組與黃芪多糖組血液中白細胞及紅細胞計數明顯迴升（ P＜0.05），且BALF中紅細胞及白細胞明顯降低（ P＜0.05）；但黃芪多糖組與地塞米鬆組比較差異無統計學意義。肺組織病理學顯示：模型組較正常組大鼠，肺泡壁破裂、肺泡間隔增寬、肺間質增生、肺泡腔及肺間質有大量炎性細胞滲齣；地塞米鬆組及黃芪多糖組大鼠均較模型組明顯減輕，但2者之間差異無統計學意義。結論黃芪多糖能顯著改善大鼠急性放射性肺損傷，作用與地塞米鬆相近，為放射性肺損傷提供瞭治療新方法。
목적：탐토황기다당대대서급성방사성폐손상적치료작용。방법48지Wistar대서수궤분성정상조、모형조、황기다당조、지새미송조，매조12지。후3조대서채용6MV-X선직선가속기대대서쌍폐진행조사（20 Gy／차）조모，수후분별급여0.9％록화납주사액、황기다당（8 mg／mL）화지새미송（0.05 mg／mL）지속관위치료8주，정상조양장조사，급여생리염수관위우방료후2、8주，매조수궤추취6지대서처사，심장취혈진행백세포、홍세포계수；취각조대서우상폐조직，HE염색관찰병리개변。결과성공구건료방사성폐손상대서모형，일반정황관찰현시：조사후적대서륙속출현정신위미，활동량감소、궁배、탈모등증상，황기다당급지새미송처리후적대서여모형조비교，탈모등현상축점개선。정상조미견임하명현이상변화。여정상조비교，모형조대서혈액중백세포급홍세포계수명현강저（P＜0.05），폐포관세액（bronchoalveolar lavage fluid，BALF）중백세포급홍세포명현승고（P＜0.05）；여모형조비교，지새미송조여황기다당조혈액중백세포급홍세포계수명현회승（ P＜0.05），차BALF중홍세포급백세포명현강저（ P＜0.05）；단황기다당조여지새미송조비교차이무통계학의의。폐조직병이학현시：모형조교정상조대서，폐포벽파렬、폐포간격증관、폐간질증생、폐포강급폐간질유대량염성세포삼출；지새미송조급황기다당조대서균교모형조명현감경，단2자지간차이무통계학의의。결론황기다당능현저개선대서급성방사성폐손상，작용여지새미송상근，위방사성폐손상제공료치료신방법。
Objective To investigate the effects of astragalus polysaccharides on radio-pulmonary lesion.Methods 48 Wistar rats were divided into normal group, model group, astragalus polysaccharides treated group and dexamethasone treated group, each had 12 rats.Models of radio-pulmonary lesion of rats in later three groups were established by whole-thorax irradiation 20 Gy, then normal group was pretending to irradiation.Rats in normal group and model group were given 0.9% NaCl, astragalus polysaccharides treated group and dexamethasone treated group were given astragalus polysaccharides(8mg/mL) and dexamethasone(0.05mg/mL), accordingly.All rats were with 8 weeks, at 2 w and 8 w post-injury, 6 rats in each group were selected randomly and anesthesia to death.The erythrocytes and leukocytes in blood and bronchoalveolar lavage fluid ( BALF) in each group were detected and histological examination of lung tissues were performed by Haematoxylin and Eosin staining to study general morphology.Results Radiation-induced lung injury rat model were successfully constructed, general observation showed that irradiated rats gradually appear listlessness, reduce the volume of activities, arched, hair removal and other symptoms, astragalus polysaccharide (APS) and dexamethasone treated rats had gradual improvement than model group.Compared with control group, erythrocytes and leukocytes counts in serum of model group at 2 w and 8 w post-injury were significantly decreased(P <0.05), the numbers of erythrocytes and leukocytes in BALF were significantly increased(P <0.05). Compared with model group group, the leukocytes, erythrocytes and neutrophils counts in serum of astragalus polysaccharides treated group and dexamethasone treated group were all increased(P <0.05), respectively, and the number of leukocytes and erythrocytes in BLAF were significantly decreased(P<0.05).All the indexes between two groups had no significant difference.The pathological changes of lung tissues showed that rats in model group had rupture alveolar wall, widened alveolar interval, pulmonary interstitial hyperplasia, and the alveolar space and interstitial lung stroma had a large number of inflammatory cells exudation.The symptoms in astragalus polysaccharides treated group and dexamethasone treated group were all alleviated, and there was no significant difference between the two groups.Conclusion Astragalus polysaccharides have therapeutic effect on radio-pulmonary lesion in rats, and the therapeutic effects of astragalus polysaccharides are roughly similar to dexamethasone.Further research is needed to elucidate the mechanism behind the effects of astragalus polysaccharides in order to develop appropriate treatment.
