CAJ | 학술논문

系统性红斑狼疮患者外周血T、B细胞自噬水平及其临床意义
계통성홍반랑창환자외주혈T、B세포자서수평급기림상의의
Levels of autophagy in T cells and B cell of patients with systemic lupus erythe-matosus and its clinical significance

万方数据

中国免疫学杂志中國免疫學雜誌 중국면역학잡지
Chinese Journal of Immunology
2015年 10期 1380-1384,1388 ,共6页

陈燕文%王超%王旭光%冯永民%安宁%吴洪銮%李尚妹%陶静莉%刘伟敬%刘华锋%潘庆军

진연문%왕초%왕욱광%풍영민%안저%오홍란%리상매%도정리%류위경%류화봉%반경군

系统性红斑狼疮%T细胞%B细胞%自噬%LC3-Ⅱ 繫統性紅斑狼瘡%T細胞%B細胞%自噬%LC3-Ⅱ
계통성홍반랑창%T세포%B세포%자서%LC3-Ⅱ
Systemic lupus erythematous%T lymphocytes%B lymphocytes%Autophagy%LC3-Ⅱ

目的：探讨系统性红斑狼疮（Systemic lupus erythematosus，SLE）患者外周血T、B 淋巴细胞自噬水平及其临床意义。方法：入组就诊前4周内未用药的SLE 患者68例；用流式细胞术检测23例正常人和68例经激素和免疫抑制剂治疗前后的SLE 患者外周血T、B 淋巴细胞的自噬水平及其变化情况，并分析其与补体C3、SLEDAI 评分和血清Anti－dsDNA 水平的相关性。结果：治疗前SLE 患者T 淋巴细胞自噬水平显著高于正常人，活动组（SLEDAI score≥10）SLE 患者显著高于稳定组（SLEDAI score＜10），且活动组中初发组（即首次确诊）显著高于复发组（P 均＜0．05）。而SLE 患者B 淋巴细胞自噬水平显著低于正常人，活动组SLE 患者显著低于稳定组，且活动组中初发组显著低于复发组（P 均＜0．05）。相关性分析发现，SLE 患者T 淋巴细胞自噬水平与SLEDAI 评分呈正相关（rs ＝0．289，P＜0．05），B 淋巴细胞自噬水平与血清补体C3水平呈正相关（rs ＝0．371，P＜0．01）。经激素和免疫抑制剂治疗5 d 后，SLE 患者预后良好组（SLEDAI 降低≥4分）的T 淋巴细胞自噬水平即可显著降低（P＜0．05）；治疗3 d 后，SLE 患者预后良好组的B 淋巴细胞自噬水平即可显著升高（P＜0．05）；预后不良组治疗前后T、B 淋巴细胞的自噬水平变化无统计学意义。结论：SLE 患者外周血T、B 淋巴细胞自噬水平异常，其变化与疾病活动度相关，有望作为疾病活动度指标和潜在的临床治疗靶标。
목적：탐토계통성홍반랑창（Systemic lupus erythematosus，SLE）환자외주혈T、B 림파세포자서수평급기림상의의。방법：입조취진전4주내미용약적SLE 환자68례；용류식세포술검측23례정상인화68례경격소화면역억제제치료전후적SLE 환자외주혈T、B 림파세포적자서수평급기변화정황，병분석기여보체C3、SLEDAI 평분화혈청Anti－dsDNA 수평적상관성。결과：치료전SLE 환자T 림파세포자서수평현저고우정상인，활동조（SLEDAI score≥10）SLE 환자현저고우은정조（SLEDAI score＜10），차활동조중초발조（즉수차학진）현저고우복발조（P 균＜0．05）。이SLE 환자B 림파세포자서수평현저저우정상인，활동조SLE 환자현저저우은정조，차활동조중초발조현저저우복발조（P 균＜0．05）。상관성분석발현，SLE 환자T 림파세포자서수평여SLEDAI 평분정정상관（rs ＝0．289，P＜0．05），B 림파세포자서수평여혈청보체C3수평정정상관（rs ＝0．371，P＜0．01）。경격소화면역억제제치료5 d 후，SLE 환자예후량호조（SLEDAI 강저≥4분）적T 림파세포자서수평즉가현저강저（P＜0．05）；치료3 d 후，SLE 환자예후량호조적B 림파세포자서수평즉가현저승고（P＜0．05）；예후불량조치료전후T、B 림파세포적자서수평변화무통계학의의。결론：SLE 환자외주혈T、B 림파세포자서수평이상，기변화여질병활동도상관，유망작위질병활동도지표화잠재적림상치료파표。
Objective:To investigate levels of autophagy in T cells and B cell of patients with systemic lupus erythematosus ( SLE) and its clinical significance.Methods: 68 SLE patients without treatment within 4 weeks were enrolled in this study.We accessed the levels of autophagy in T cells and B cells of 23 healthy controls and 68 patients before and after treatment by flow cytometry,and analyzed their correlations with serum levels of C3 and anti-dsDNA antibodies,SLEDAI score,et al.Results: Before treatment,a significantly increased levels of LC3-Ⅱ was observed in SLE patients than healthy controls, the active group ( SLEDAI score≥10) was significantly higher than the stable group(SLEDAI score<10),and the newly diagnosed group was significantly higher than the recurrent group(all P<0.05).While the levels of LC3-Ⅱ was decreased in B lymphocytes in SLE patients,the active group was significantly lower than stable group,and the active group was significantly lower than the newly diagnosed group(all P<0.05). Correlation analysis found that,a positively correlation was observed for the levels of LC3-Ⅱwith SLEDAI score in T lymphocyte( rs=0.289,P<0.05),and the levels of C3 were positively correlated the levels of LC3-Ⅱ in B lymphocyte(rs=0.371,P<0.01).After treatment for five days, levels of autophagy in T lymphocytes of SLE patients with good prognosis ( SLEDAI score decreased ≥4 ) significantly decreased(P<0.05).Also,three days after treatment,levels of autophagy in B lymphocytes of SLE patients with good prognosis were increased significantly ( P<0.05 ) .However, SLE patients with poor prognosis had no such difference ( P>0.05 ) . Conclusion:Levels of autophagy in T and B lymphocytes of SLE patients are abnormal compared to healthy controls,and these changes are associated with disease activity.Also,these changes are expected to be the indicators of disease activity and potential therapeutic targets in SLE.