CAJ | 학술논문

采用蒸发光散射高效液相色谱法测定黄芪饮片中黄芪甲苷的含量。黄芪饮片分别用甲醇和正丁醇提取，以NaOH溶液洗涤，蒸干，再用甲醇溶解，采用Agilent SB–C18柱(250 mm×4.6 mm，5μm)分离测定，流动相为乙腈–水(32∶68)，流量为1.0 mL／min，漂移管温度为80℃。方法检出限为1.5μg／mL，黄芪甲苷溶液的质量浓度在2～12μg／mL范围内与色谱峰面积线性关系良好(r2=0.9996)，测定结果的相对标准偏差为0.65%（n=6），平均加标回收率为99.6%。该方法稳定可靠，可用于黄芪药材及其生物制品中黄芪甲苷含量的测定。
채용증발광산사고효액상색보법측정황기음편중황기갑감적함량。황기음편분별용갑순화정정순제취，이NaOH용액세조，증간，재용갑순용해，채용Agilent SB–C18주(250 mm×4.6 mm，5μm)분리측정，류동상위을정–수(32∶68)，류량위1.0 mL／min，표이관온도위80℃。방법검출한위1.5μg／mL，황기갑감용액적질량농도재2～12μg／mL범위내여색보봉면적선성관계량호(r2=0.9996)，측정결과적상대표준편차위0.65%（n=6），평균가표회수솔위99.6%。해방법은정가고，가용우황기약재급기생물제품중황기갑감함량적측정。
HPLC–ELSD method was used to determine astragalosideⅣ in astraglus decoction pieces. The sample of astragalous decoction pieces was extracted by methanol andn-butyl alchol, after cleaning with NaOH solution, the sample was dried and disolved in methanol, and was determined by Agilent SB–C18 column (250 mm×4.6 mm, 5μm), acetonitrile–water(32∶68) was as the mobile with theflow rate of 1.0 mL/min, the drifttube temperature was 80℃. The detection limit of the method was 1.5μg/mL. The linear range was 2–12μg/mL(r2=0.999 6). The RSD of determination results was 0.65%(n=6). The average recovery of astragalosideⅣ was 99.6%. This method is accurate, reliable and with good reproducibility, and it can be used for the determination of astragalosideⅣ in Radix Astragali and its products.