检验医学与临床
檢驗醫學與臨床
검험의학여림상
Laboratory Medicine and Clinic
2015年
20期
2991-2993
,共3页
傅蔷%林晓岚%陈万紫%陈加弟%黄慧芳
傅薔%林曉嵐%陳萬紫%陳加弟%黃慧芳
부장%림효람%진만자%진가제%황혜방
慢性淋巴细胞白血病%荧光原位杂交%分子遗传学
慢性淋巴細胞白血病%熒光原位雜交%分子遺傳學
만성림파세포백혈병%형광원위잡교%분자유전학
chronic lymphocytic leukemia%FISH%molecular genetics
目的:探讨间期荧光原位杂交(FISH)技术检测慢性淋巴细胞白血病(CLL)常见分子遗传学异常的意义。方法采用CSF12(12p11.1~12q11.1)、D13S25(13q14.3)、RB1(13q14)、ATM(11q22.3)及p53(17p13.1)等5种探针对59例初诊CLL患者的骨髓细胞进行间期FISH检测,分析上述分子遗传学异常与患者的临床Binet分期、Ria分期及相关实验室检查,包括初诊时外周血淋巴细胞绝对值计数、血红蛋白(Hb)水平、血小板计数(PLT),血清乳酸脱氢酶(LDH)、β2‐微球蛋白(β2‐MG)、CD38和ZAP70水平的相关性。结果59例CLL初诊患者中,存在1种及1种以上分子遗传学异常的有49例,占83.1%。其中,del(13q)34例(69.4%),12号染色体三体(+12)和p53基因缺失各11例(22.4%),ATM基因缺失5例(10.2%)。各分子遗传学异常与患者临床分期、外周血淋巴细胞计数绝对值、Hb、PLT、LDH、β2‐MG、CD38和ZAP70表达水平之间均无明显相关性(P>0.05)。≥60岁患者del(13q)异常率(67.6%)显著高于<60岁患者(40.9%),女性患者p53基因缺失的异常率(35.2%)显著高于男性患者(11.9%),差异均有统计学意义(P<0.05)。结论FISH技术是检测CLL患者分子遗传学异常快速、准确、敏感的方法,分子遗传学异常与患者初诊时临床表现之间无明显相关性。
目的:探討間期熒光原位雜交(FISH)技術檢測慢性淋巴細胞白血病(CLL)常見分子遺傳學異常的意義。方法採用CSF12(12p11.1~12q11.1)、D13S25(13q14.3)、RB1(13q14)、ATM(11q22.3)及p53(17p13.1)等5種探針對59例初診CLL患者的骨髓細胞進行間期FISH檢測,分析上述分子遺傳學異常與患者的臨床Binet分期、Ria分期及相關實驗室檢查,包括初診時外週血淋巴細胞絕對值計數、血紅蛋白(Hb)水平、血小闆計數(PLT),血清乳痠脫氫酶(LDH)、β2‐微毬蛋白(β2‐MG)、CD38和ZAP70水平的相關性。結果59例CLL初診患者中,存在1種及1種以上分子遺傳學異常的有49例,佔83.1%。其中,del(13q)34例(69.4%),12號染色體三體(+12)和p53基因缺失各11例(22.4%),ATM基因缺失5例(10.2%)。各分子遺傳學異常與患者臨床分期、外週血淋巴細胞計數絕對值、Hb、PLT、LDH、β2‐MG、CD38和ZAP70錶達水平之間均無明顯相關性(P>0.05)。≥60歲患者del(13q)異常率(67.6%)顯著高于<60歲患者(40.9%),女性患者p53基因缺失的異常率(35.2%)顯著高于男性患者(11.9%),差異均有統計學意義(P<0.05)。結論FISH技術是檢測CLL患者分子遺傳學異常快速、準確、敏感的方法,分子遺傳學異常與患者初診時臨床錶現之間無明顯相關性。
목적:탐토간기형광원위잡교(FISH)기술검측만성림파세포백혈병(CLL)상견분자유전학이상적의의。방법채용CSF12(12p11.1~12q11.1)、D13S25(13q14.3)、RB1(13q14)、ATM(11q22.3)급p53(17p13.1)등5충탐침대59례초진CLL환자적골수세포진행간기FISH검측,분석상술분자유전학이상여환자적림상Binet분기、Ria분기급상관실험실검사,포괄초진시외주혈림파세포절대치계수、혈홍단백(Hb)수평、혈소판계수(PLT),혈청유산탈경매(LDH)、β2‐미구단백(β2‐MG)、CD38화ZAP70수평적상관성。결과59례CLL초진환자중,존재1충급1충이상분자유전학이상적유49례,점83.1%。기중,del(13q)34례(69.4%),12호염색체삼체(+12)화p53기인결실각11례(22.4%),ATM기인결실5례(10.2%)。각분자유전학이상여환자림상분기、외주혈림파세포계수절대치、Hb、PLT、LDH、β2‐MG、CD38화ZAP70표체수평지간균무명현상관성(P>0.05)。≥60세환자del(13q)이상솔(67.6%)현저고우<60세환자(40.9%),녀성환자p53기인결실적이상솔(35.2%)현저고우남성환자(11.9%),차이균유통계학의의(P<0.05)。결론FISH기술시검측CLL환자분자유전학이상쾌속、준학、민감적방법,분자유전학이상여환자초진시림상표현지간무명현상관성。
Objective To investigate the significance of interphase fluorescence in situ hybridization (FISH ) technology for detecting common molecular cytogenetic abnormalities in the patients with chronic lymphocytic leuke‐mia(CLL) .Methods The bone marrow cells from 59 patients with newly diagnosed CLL were performed the inter‐phase FISH detection by 5 probes ,including CSF12(12p11 .1 -12q11 .1) ,D13S25(13q14 .3) ,RB1(13q14) ,ATM (11q22 .3) and p53(17p13 .1) .Then the correlation between the molecular cytogenetic abnormalities and the clinical Binet staging ,Rai staging and the related laboratory examinations including peripheral blood lymphocyte absolute count ,Hb ,PLT ,LDH ,β2‐MG ,ZAP70 and CD38 levels were analyzed .Results Among 59 cases of newly diagnosed CLL ,49 cases(83 .1% ) had one and more than one kind of molecular cytogenetic aberrations ,in which del(13q) was 34 cases(69 .4% ) ,each of +12 and del(p53) was 11 cases(22 .4% ) and del(ATM ) was 5 cases(10 .2% ) .The mo‐lecular cytogenetic abnormalities had no obvious correlation with the Binet staging ,Rai staging ,peripheral lymphocyte absolute count ,Hb ,PLT ,serum levels of LDH ,β2‐MG ,ZAP70 and CD38(P> 0 .05) .The abnormality rate of del (13q) in the over 60 years old group was significantly higher than that in the less than 60 years old group(P=0 .03) , and the abnormality rate of p53 deletion in female was higher than that in male ,the differences were statistically sig‐nificant(P<0 .05) .Conclusion The FISH technology is a rapid ,accurate and sensitive method in detecting molecular cytogenetic abnormalities in CLL patients ,and there is no significant correlation between molecular cytogenetic ab‐normalities and clinical manifestations in first visiting hospital .