国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
International Journal of Laboratory Medicine
2015年
19期
2840-2841,2843
,共3页
江秀爱%赵自云%姜蓓%乔显森
江秀愛%趙自雲%薑蓓%喬顯森
강수애%조자운%강배%교현삼
嗜血杆菌%耐药性%药物敏感试验%β-内酰胺酶%生物学分型
嗜血桿菌%耐藥性%藥物敏感試驗%β-內酰胺酶%生物學分型
기혈간균%내약성%약물민감시험%β-내선알매%생물학분형
Haemophitus%drug resistance%antimicrobial susceptibility%β-laetamase%biotyping
目的：了解该院临床分离嗜血杆菌属的季节分布、生物学分型及耐药性。方法收集呼吸道感染患者的痰液和咽拭子，采用手工法、MicSCAN4全自动细菌鉴定分析仪、HNID 鉴定板对分离培养的221株嗜血杆菌进行菌种鉴定，并进行生物学分型。用纸片扩散(K-B)法进行药物敏感试验，采用头孢硝噻吩纸片法进行β-内酰胺酶检测。结果临床分离流感嗜血杆菌96株(占1.6%)，生物Ⅰ型10株(10.4%)，生物Ⅱ型31株(32.3%)，生物Ⅲ型40株(41.7%)，其他生物型15株(15.6%)。副流感嗜血杆菌125株(占2.1%)，生物Ⅰ型15株(12.0%)，生物Ⅱ型23株(18.4%)，生物Ⅲ型69株(55.2%)，生物Ⅳ型18株(14.4%)，未检出其他生物型。冬季感染率最高。流感嗜血杆菌和副流感嗜血杆菌对氨苄西林耐药率分别为40.6%%和44.8%，对复方磺胺甲噁唑的耐药率分别为51.0%和66.4%。流感嗜血杆菌和副流感嗜血杆菌β-内酰胺酶株产酶率分别为40.6%和44.8%，对头孢呋辛、头孢噻肟、美罗培南、氯霉素的敏感率都在90.0%以上。结论呼吸道流感嗜血杆菌和副流感嗜血杆菌感染好发于冬季，感染的生物型均以生物Ⅱ型、Ⅲ型为主，对氨苄西林和复方磺胺甲噁唑的耐药率较高，已不宜用于嗜血杆菌引起感染的经验治疗。对该菌所致呼吸道感染可选用头孢呋辛、头孢噻肟、美罗培南作为治疗的首选药物。
目的：瞭解該院臨床分離嗜血桿菌屬的季節分佈、生物學分型及耐藥性。方法收集呼吸道感染患者的痰液和嚥拭子，採用手工法、MicSCAN4全自動細菌鑒定分析儀、HNID 鑒定闆對分離培養的221株嗜血桿菌進行菌種鑒定，併進行生物學分型。用紙片擴散(K-B)法進行藥物敏感試驗，採用頭孢硝噻吩紙片法進行β-內酰胺酶檢測。結果臨床分離流感嗜血桿菌96株(佔1.6%)，生物Ⅰ型10株(10.4%)，生物Ⅱ型31株(32.3%)，生物Ⅲ型40株(41.7%)，其他生物型15株(15.6%)。副流感嗜血桿菌125株(佔2.1%)，生物Ⅰ型15株(12.0%)，生物Ⅱ型23株(18.4%)，生物Ⅲ型69株(55.2%)，生物Ⅳ型18株(14.4%)，未檢齣其他生物型。鼕季感染率最高。流感嗜血桿菌和副流感嗜血桿菌對氨芐西林耐藥率分彆為40.6%%和44.8%，對複方磺胺甲噁唑的耐藥率分彆為51.0%和66.4%。流感嗜血桿菌和副流感嗜血桿菌β-內酰胺酶株產酶率分彆為40.6%和44.8%，對頭孢呋辛、頭孢噻肟、美囉培南、氯黴素的敏感率都在90.0%以上。結論呼吸道流感嗜血桿菌和副流感嗜血桿菌感染好髮于鼕季，感染的生物型均以生物Ⅱ型、Ⅲ型為主，對氨芐西林和複方磺胺甲噁唑的耐藥率較高，已不宜用于嗜血桿菌引起感染的經驗治療。對該菌所緻呼吸道感染可選用頭孢呋辛、頭孢噻肟、美囉培南作為治療的首選藥物。
목적：료해해원림상분리기혈간균속적계절분포、생물학분형급내약성。방법수집호흡도감염환자적담액화인식자，채용수공법、MicSCAN4전자동세균감정분석의、HNID 감정판대분리배양적221주기혈간균진행균충감정，병진행생물학분형。용지편확산(K-B)법진행약물민감시험，채용두포초새분지편법진행β-내선알매검측。결과림상분리류감기혈간균96주(점1.6%)，생물Ⅰ형10주(10.4%)，생물Ⅱ형31주(32.3%)，생물Ⅲ형40주(41.7%)，기타생물형15주(15.6%)。부류감기혈간균125주(점2.1%)，생물Ⅰ형15주(12.0%)，생물Ⅱ형23주(18.4%)，생물Ⅲ형69주(55.2%)，생물Ⅳ형18주(14.4%)，미검출기타생물형。동계감염솔최고。류감기혈간균화부류감기혈간균대안변서림내약솔분별위40.6%%화44.8%，대복방광알갑오서적내약솔분별위51.0%화66.4%。류감기혈간균화부류감기혈간균β-내선알매주산매솔분별위40.6%화44.8%，대두포부신、두포새우、미라배남、록매소적민감솔도재90.0%이상。결론호흡도류감기혈간균화부류감기혈간균감염호발우동계，감염적생물형균이생물Ⅱ형、Ⅲ형위주，대안변서림화복방광알갑오서적내약솔교고，이불의용우기혈간균인기감염적경험치료。대해균소치호흡도감염가선용두포부신、두포새우、미라배남작위치료적수선약물。
Objective To investigate season distribution,biological typing and drug resistant of Haemophitus in Qingdao Central Hospital.Methods The sputum and throat swab were collected from patients with respiratory tract infection,221 Haemophilus strains were identified and typed by the manual method and MicSCAN4 automatic analyzer,HNID identification plate.Antimicrobial susceptibility was tested by Kirby-Bauer method,and cephalosporins nitrate thiophene paper method was used to detect β-lacta-mase.Results A total of 96 strains of Haemophilus influenzae(1.6%)were isolated,10(10.4%)strains of Haemophilus influenzae were identified as type Ⅰ,31(32.3%)as type Ⅱ,40(41.7%)as typeⅢand 1 5(1 5.6%)as other types.A total of 125 strains Hae-mophitus parl influenzae(2.1%)were isolated,1 5 (12.0%)strains of Haemophilus parl influenzae were identified as type Ⅰ,23 (18.4%)as typeⅡ,69(55.2%)as type Ⅲ and 18(14.4%)as type Ⅳ,other types were not identified.The highest infected rate was in winter.Resistance rate of Haemophilus influenzae and Haemophitus parl influenzae to ampicillin were 40.6% and 44.8%,to tri-methoprim-sulfamethoxazole were 5 1.0% and 66.4%.The prevalence ofβ-lactamase of all strains were 40.6%and 44.8%.But sus-ceptible rates of Haemophilus to cefotaxime,cefuroxime,meropenem,chloramphenicol were over 90.0%.Conclusion The respira-tory tract infections to Haemophilus influenzae and Haemophitus parl influenzae is more frequently found in winter.Type Ⅱ and type Ⅲ are the most prevalent types.The resistance rates of Haemophilus to ampicillin and trimethoprim-sulfamethoxazole are in-creasing,should not be used as empirical treatment of Haemophilus infection.Antibiotics such as cefotaxime,cefuroxime,meropen-em could be chosen for the treatment of respiratory tract infection caused by Haemophilus.
