CAJ | 학술논문

目的：观察肝硬化对大鼠心肌转化生长因子β1(transforming growth factor-β1,TGF-β1)及胶原Ⅰ(collagenⅠ,ColⅠ)基因表达的影响及促红细胞生成素的干预作用。方法清洁级SD大鼠36只,随机分为3组：正常对照组、肝硬化组及EPO干预组。在体测定各组心脏血流动力学指标,测血清乳酸脱氢酶( LDH)及肌酸激酶同工酶( CK-MB)水平,Masson染色法观察各组大鼠心肌胶原形成情况, RT-PCR法检测各组心肌 TGF-β1、ColⅠmRNA 基因表达的变化。结果与正常对照组相比,肝硬化组大鼠左心室收缩和舒张功能下降,心肌酶增高,心肌胶原沉积明显增加,心肌TGF-β1及ColⅠ基因表达升高。与肝硬化组相比,EPO干预组大鼠心室收缩和舒张功能提高,心肌酶指标降低,心肌胶原沉积改善,心肌TGF-β1及ColⅠ基因表达降低。结论肝硬化能引起大鼠心肌结构和功能的改变,促进心肌间质纤维化的发生；EPO对肝硬化大鼠心肌损伤有一定的保护作用。
목적：관찰간경화대대서심기전화생장인자β1(transforming growth factor-β1,TGF-β1)급효원Ⅰ(collagenⅠ,ColⅠ)기인표체적영향급촉홍세포생성소적간예작용。방법청길급SD대서36지,수궤분위3조：정상대조조、간경화조급EPO간예조。재체측정각조심장혈류동역학지표,측혈청유산탈경매( LDH)급기산격매동공매( CK-MB)수평,Masson염색법관찰각조대서심기효원형성정황, RT-PCR법검측각조심기 TGF-β1、ColⅠmRNA 기인표체적변화。결과여정상대조조상비,간경화조대서좌심실수축화서장공능하강,심기매증고,심기효원침적명현증가,심기TGF-β1급ColⅠ기인표체승고。여간경화조상비,EPO간예조대서심실수축화서장공능제고,심기매지표강저,심기효원침적개선,심기TGF-β1급ColⅠ기인표체강저。결론간경화능인기대서심기결구화공능적개변,촉진심기간질섬유화적발생；EPO대간경화대서심기손상유일정적보호작용。
Aim To observe the effects of liver cirrho-sis on the expression of transforming growth factor-β1 ( TGF-β1 ) and ColⅠin rat myocardium and interven-tion of erythropoietin ( EPO ) . Methods Thirty-six male Sprague-Dasley rats were randomly divided into three groups:control group, liver cirrhosis group and EPO group, then the cardic hemodynamic parameters in vivo and levels of serum lactate dehydrogenase ( LDH ) as well as creatine kinase isoenzyme ( CK-MB) were measured. With Masson′s trichrome stain, changes of collagen formation of myocardial tissue in different groups were observed. Also the mRNA ex-pressions of TGF-β1 and ColⅠin myocardium were de-tected by RT-PCR. Results In contrast to control group, rats in liver cirrhosis group showed a decline in systolic and diastolic function of left ventricule, rising myocardial enzyme, a distinct increase of cardiac colla-gen deposition, as well as an elevation of TGF-β1 and ColⅠmRNA expressions. In contrast to liver cirrhosis group, rats in EPO group demonstrated an improve-ment in systolic and diastolic function of left ventricule as well as in cardiac collagen deposition, and a de-crease in both myocardial enzyme and TGF-β1 and ColⅠmRNA expressions. Conclusion Liver cirrhosis can lead to the changes of myocardial structure and function in rats,and it can accelerate myocardial inter-stitial fibrosis; EPO can protect the myocardial injury in liver cirrhosis rats.