CAJ | 학술논문

目的：比较 ELISA法和间接免疫荧光(IIF)法诊断自身免疫疾病的价值。方法系统性红斑狼疮(SLE)组33例,非SLE 自身免疫疾病组59例,非自身免疫疾病组43例,同期该院体检中心健康体检者20例纳入健康对照组。采用2种方法检测4组研究对象的抗核抗体(ANA),并进行分析。结果ELISA 法检测 SLE 组、非 SLE 自身免疫疾病组中高效价的 ANA 分别为(2.621±1.700)、(2.248±1.781),IIF 法分别为(2.715±0.730)、(2.544±0.59),但前者测出非自身免疫疾病中的 ANA (1.034±1.050)低于后者 ANA 的效价(2.253±0.691)。结论ELISA 检测可提高 ANA 的检测效果。
목적：비교 ELISA법화간접면역형광(IIF)법진단자신면역질병적개치。방법계통성홍반랑창(SLE)조33례,비SLE 자신면역질병조59례,비자신면역질병조43례,동기해원체검중심건강체검자20례납입건강대조조。채용2충방법검측4조연구대상적항핵항체(ANA),병진행분석。결과ELISA 법검측 SLE 조、비 SLE 자신면역질병조중고효개적 ANA 분별위(2.621±1.700)、(2.248±1.781),IIF 법분별위(2.715±0.730)、(2.544±0.59),단전자측출비자신면역질병중적 ANA (1.034±1.050)저우후자 ANA 적효개(2.253±0.691)。결론ELISA 검측가제고 ANA 적검측효과。
Objective To compare the value of ELISA and indirect immunofluorescence (IIF)method in diagnosis for autoim-mune diseases.Methods A total of 33 patients in systemic lupus erythematosus(SLE)group,59 patients in other autoimmune dis-eases group,43 patients in non-autoimmune disease group,20 people accepted physical examination in control group.The antinuclear antibody (ANA)in each group were detected by two methods and analyzed.Results The high titer ANA detected by ELISA and IIF in SLE group and other autoimmune diseases group were (2.621±1.700),(2.248±1.781);(2.71 5±0.730),(2.544±0.59). The titer ANA detected by ELISA in non-autoimmune disease group was (1.034±1.050),which was lower than(2.253 ±0.691) detected by IIF.Conclusion ELSA might improve the detection effects of ANA antibody.