中国医药导报
中國醫藥導報
중국의약도보
China Medical Herald
2015年
27期
9-13
,共5页
苏海洋%申俊峰%罗雷雷%胡志强
囌海洋%申俊峰%囉雷雷%鬍誌彊
소해양%신준봉%라뢰뢰%호지강
动静脉畸形%模型%E-选择素%P-选择素%细胞间黏附分子-1%血管细胞黏附分子-1%组织因子
動靜脈畸形%模型%E-選擇素%P-選擇素%細胞間黏附分子-1%血管細胞黏附分子-1%組織因子
동정맥기형%모형%E-선택소%P-선택소%세포간점부분자-1%혈관세포점부분자-1%조직인자
AVM%Model%E-selectin%P-selectin%ICAM-1%VCAM-1%TF
目的：探索鼠模型动静脉畸形在放射外科治疗后血清中炎症分子的改变,为进一步寻找人类脑动静脉畸形对放射外科治疗反应的早期监测标志物奠定基础。方法将12只雄性SD大鼠随机分成实验组和对照组,每组6只。利用显微血管吻合法进行造模,造模后42 d,利用酶联免疫吸附试验分别定量测定两组大鼠放疗前血清中可溶性E-选择素、P-选择素、细胞间黏附分子-1(SICAM-1)、血管细胞黏附分子-1(SVCAM-1)、组织因子及实验组放疗后6、12、24、48、72、120 h血清中以上各因子的含量,并利用SPSS17.0统计学软件进行数据分析。结果放疗后实验组大鼠血清可溶性E-选择素及P-选择素、SICAM-1、SVCAM-1及组织因子较放疗前及对照组升高。实验组放疗不同时间点各种炎症分子的表达有差异：放疗后6、12 h时,实验组血清中可溶性E-选择素的平均浓度较放疗前显著升高(P<0.05)；放疗后6、12、24、48、72 h时血清中可溶性P-选择素的水平与放疗前比较显著升高(P<0.05)；放疗后6 h和12 h时,血清中sICAM-1和sVCAM-1的浓度较放疗前均显著升高(P<0.05)；放疗后6 h血浆可溶性组织因子浓度较放疗前升高1倍,在放疗后24 h内维持在高水平(P<0.05)。结论在放射外科治疗动静脉畸形的过程中在不同时间点对以上炎症分子进行组合监测可作为早期预测动静脉畸形对于放射外科治疗反应的标志物。
目的：探索鼠模型動靜脈畸形在放射外科治療後血清中炎癥分子的改變,為進一步尋找人類腦動靜脈畸形對放射外科治療反應的早期鑑測標誌物奠定基礎。方法將12隻雄性SD大鼠隨機分成實驗組和對照組,每組6隻。利用顯微血管吻閤法進行造模,造模後42 d,利用酶聯免疫吸附試驗分彆定量測定兩組大鼠放療前血清中可溶性E-選擇素、P-選擇素、細胞間黏附分子-1(SICAM-1)、血管細胞黏附分子-1(SVCAM-1)、組織因子及實驗組放療後6、12、24、48、72、120 h血清中以上各因子的含量,併利用SPSS17.0統計學軟件進行數據分析。結果放療後實驗組大鼠血清可溶性E-選擇素及P-選擇素、SICAM-1、SVCAM-1及組織因子較放療前及對照組升高。實驗組放療不同時間點各種炎癥分子的錶達有差異：放療後6、12 h時,實驗組血清中可溶性E-選擇素的平均濃度較放療前顯著升高(P<0.05)；放療後6、12、24、48、72 h時血清中可溶性P-選擇素的水平與放療前比較顯著升高(P<0.05)；放療後6 h和12 h時,血清中sICAM-1和sVCAM-1的濃度較放療前均顯著升高(P<0.05)；放療後6 h血漿可溶性組織因子濃度較放療前升高1倍,在放療後24 h內維持在高水平(P<0.05)。結論在放射外科治療動靜脈畸形的過程中在不同時間點對以上炎癥分子進行組閤鑑測可作為早期預測動靜脈畸形對于放射外科治療反應的標誌物。
목적：탐색서모형동정맥기형재방사외과치료후혈청중염증분자적개변,위진일보심조인류뇌동정맥기형대방사외과치료반응적조기감측표지물전정기출。방법장12지웅성SD대서수궤분성실험조화대조조,매조6지。이용현미혈관문합법진행조모,조모후42 d,이용매련면역흡부시험분별정량측정량조대서방료전혈청중가용성E-선택소、P-선택소、세포간점부분자-1(SICAM-1)、혈관세포점부분자-1(SVCAM-1)、조직인자급실험조방료후6、12、24、48、72、120 h혈청중이상각인자적함량,병이용SPSS17.0통계학연건진행수거분석。결과방료후실험조대서혈청가용성E-선택소급P-선택소、SICAM-1、SVCAM-1급조직인자교방료전급대조조승고。실험조방료불동시간점각충염증분자적표체유차이：방료후6、12 h시,실험조혈청중가용성E-선택소적평균농도교방료전현저승고(P<0.05)；방료후6、12、24、48、72 h시혈청중가용성P-선택소적수평여방료전비교현저승고(P<0.05)；방료후6 h화12 h시,혈청중sICAM-1화sVCAM-1적농도교방료전균현저승고(P<0.05)；방료후6 h혈장가용성조직인자농도교방료전승고1배,재방료후24 h내유지재고수평(P<0.05)。결론재방사외과치료동정맥기형적과정중재불동시간점대이상염증분자진행조합감측가작위조기예측동정맥기형대우방사외과치료반응적표지물。
Objective To study serum inflammatory molecular changes in response to radiosurgery in rat model of ar-teriovenous malformation, and to find the early and effective marker to detect responsiveness after using radiosurgery for human AVMs. Methods 12 male SD rats were randomly divided into experimental group and control group, 6 rats in each group. The rat models of AVMs were built by microvascular anastomosis method, 42 days after modeling, solu-ble E-selectin and P-selectin, ICAM-1, VCAM-1 and tissue factor in animal model of AVMs were quantified by ELISA before radiosurgery of rats in two groups and 6, 12, 24, 48, 72, 120 hours after radiosurgery of rats in experi-mental group, and data were analyzed by SPSS 17.0 statistical software. Results Compared with the control group and experimental group before radiotherapy, the serum concentrations of soluble E-selectin and P-selectin, SICAM-1, SV-CAM-1, and tissue factor of the rats in the experimental group were significantly increased. The expression of inflam-matory molecular changes at different time point in rats of the experimental group had statistically significant differ-ence:serum concentrations of soluble E-selectin were significantly increased at 6 and 12 hours after radiosurgery (P<0.05); serum levels of soluble P-selectin were significantly increased at 6,12, 24, 48, 72 hours after radiosurgery (P<0.05); serum concentrations of soluble ICAM-1 and VCAM-1 were significantly increased at 6 and 12 hours after ra-diosurgery (P < 0.05); serum concentrations of soluble tissue factor were doubled at 6 hours after radiosurgery and maintained at high levels for 24 hours (P<0.05). Conclusion A combination of these molecules measured at different time points may serve as an early predictor of responsiveness of AVMs to radiosurgery.
