CAJ | 학술논문

目的：精确鉴定醋酸钙-鲍曼不动杆菌复合菌菌种，了解第三军医大学大坪医院醋酸钙-鲍曼不动杆菌复合菌的菌种分布情况；比较分析各菌种对临床常用抗菌药物的体外敏感率。方法选取2014年2~4月该院临床分离的95株醋酸钙-鲍曼不动杆菌复合菌非重复菌株，采用16S rRNA 基因测序法鉴定所有菌株到种；利用 VITEK-2 Compact 自动细菌鉴定药敏仪检测所有细菌对临床常用抗菌药物的体外敏感率。结果95株醋酸钙-鲍曼不动杆菌复合菌精确鉴定为鲍曼不动杆菌81株(85.26%)、皮特不动杆菌10株(10.53%)、医院不动杆菌4株(4.21%)。药敏试验结果显示，鲍曼不动杆菌对三代头孢菌素、氨基糖苷类和碳青霉烯类抗菌药物耐药情况严重，敏感率在20.00%左右。而皮特不动杆菌和医院不动杆菌对常用抗菌药物的敏感率都较高。结论传统的生化鉴定方法在不动杆菌种属鉴定中存在局限性，利用16S rRNA 基因测序分析，就可以精确鉴定醋酸钙-鲍曼不动杆菌复合菌，复合菌中各菌种的耐药特性存在较大差异。
목적：정학감정작산개-포만불동간균복합균균충，료해제삼군의대학대평의원작산개-포만불동간균복합균적균충분포정황；비교분석각균충대림상상용항균약물적체외민감솔。방법선취2014년2~4월해원림상분리적95주작산개-포만불동간균복합균비중복균주，채용16S rRNA 기인측서법감정소유균주도충；이용 VITEK-2 Compact 자동세균감정약민의검측소유세균대림상상용항균약물적체외민감솔。결과95주작산개-포만불동간균복합균정학감정위포만불동간균81주(85.26%)、피특불동간균10주(10.53%)、의원불동간균4주(4.21%)。약민시험결과현시，포만불동간균대삼대두포균소、안기당감류화탄청매희류항균약물내약정황엄중，민감솔재20.00%좌우。이피특불동간균화의원불동간균대상용항균약물적민감솔도교고。결론전통적생화감정방법재불동간균충속감정중존재국한성，이용16S rRNA 기인측서분석，취가이정학감정작산개-포만불동간균복합균，복합균중각균충적내약특성존재교대차이。
Objective To identify strains of Acinetobacter(A.)calcoaceticus-A.baumannii complex accurately,and investigate the species distribution of Acinetobacter calcoaceticus-A.baurnannii(ACB)complex isolated in Daping Hospital.Analysis the anti-biotics resistance of ACB complex.Methods A total of 95 clinica1 isolates of ACB complex were collected from Daping Hospital from February to April 2014,identify by sequence analysis of 1 6S rRNA gene spacer region.Use VITEK-2compact automatic iden-tification of bacteria by susceptibility meter to detect the precise identification of all ACB complex group of drug sensitivity.Results Among all the 95 strains of ACB complex identification results were A.baumanni (81,85.26%),A.pittii(10,10.53%),A.noso-comialis (4,4.21%).A.baumannii to third generation cephalosporins,aminoglycosides and carbapenem antibiotic resistance in seri-ous condition,the sensitive rate was only about 20.00%.But A.pittii and A.nosocomialis had a high sensitivity rate to commonly used antibiotics.Conclusion VITEK-2 Compact automatic identification of bacteria susceptibility meter has limitations in identifica-tion of Acinetobacter,with analysis of 1 6S rRNA gene sequencing,the ACB complex could be accurately identified.There is signifi-cant difference in the composite group-resistant characteristics of each species,clinical infection is also various.