国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
International Journal of Laboratory Medicine
2015年
19期
2800-2802
,共3页
冯琳涵%徐修礼%刘家云%郝晓柯
馮琳涵%徐脩禮%劉傢雲%郝曉柯
풍림함%서수례%류가운%학효가
血培养%病原菌%抗菌药物%耐药性
血培養%病原菌%抗菌藥物%耐藥性
혈배양%병원균%항균약물%내약성
blood culture%pathogens%antimicrobial agents%drug resistance
目的：动态监测该院2007~2013年临床送检血培养标本中分离的病原菌分布及其耐药性变迁，为临床合理应用抗菌药物提供实验室依据。方法采用 BACTEC 全自动血培养仪对63229份血液标本进行检测；细菌鉴定应用全自动细菌鉴定分析仪鉴定到种；细菌药敏试验采用纸片扩散(K-B)法，按照美国临床和实验室标准化协会(CLSI)规定的标准进行；病原菌分布和药敏试验结果应用 Whonet5.6软件进行统计分析。结果从临床送检的63229份血液标本中，培养阳性4914份，阳性率7.77%，共检出病原菌2196株，革兰阴性菌主要为大肠埃希菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌，检出率分别为25.50%、11.47%、7.14%、5.91%。革兰阳性菌中以凝固酶阴性葡萄球菌、金黄色葡萄球菌、肠球菌、草绿色链球为主，分离率分别为10.40%、7.42%、6.87%、3.91%。真菌分离率为7.40%(164/2196)，产超广谱β-内酰胺酶的大肠埃希菌和肺炎克雷伯菌分离率分别为75.53%、55.51%。结论血流感染病原菌主要为大肠埃希菌、肺炎克雷伯菌、凝固酶阴性葡萄球菌、金黄色葡萄球菌，临床应严格按血培养标本的操作规范执行，避免污染并提高送检质量。
目的：動態鑑測該院2007~2013年臨床送檢血培養標本中分離的病原菌分佈及其耐藥性變遷，為臨床閤理應用抗菌藥物提供實驗室依據。方法採用 BACTEC 全自動血培養儀對63229份血液標本進行檢測；細菌鑒定應用全自動細菌鑒定分析儀鑒定到種；細菌藥敏試驗採用紙片擴散(K-B)法，按照美國臨床和實驗室標準化協會(CLSI)規定的標準進行；病原菌分佈和藥敏試驗結果應用 Whonet5.6軟件進行統計分析。結果從臨床送檢的63229份血液標本中，培養暘性4914份，暘性率7.77%，共檢齣病原菌2196株，革蘭陰性菌主要為大腸埃希菌、肺炎剋雷伯菌、鮑曼不動桿菌、銅綠假單胞菌，檢齣率分彆為25.50%、11.47%、7.14%、5.91%。革蘭暘性菌中以凝固酶陰性葡萄毬菌、金黃色葡萄毬菌、腸毬菌、草綠色鏈毬為主，分離率分彆為10.40%、7.42%、6.87%、3.91%。真菌分離率為7.40%(164/2196)，產超廣譜β-內酰胺酶的大腸埃希菌和肺炎剋雷伯菌分離率分彆為75.53%、55.51%。結論血流感染病原菌主要為大腸埃希菌、肺炎剋雷伯菌、凝固酶陰性葡萄毬菌、金黃色葡萄毬菌，臨床應嚴格按血培養標本的操作規範執行，避免汙染併提高送檢質量。
목적：동태감측해원2007~2013년림상송검혈배양표본중분리적병원균분포급기내약성변천，위림상합리응용항균약물제공실험실의거。방법채용 BACTEC 전자동혈배양의대63229빈혈액표본진행검측；세균감정응용전자동세균감정분석의감정도충；세균약민시험채용지편확산(K-B)법，안조미국림상화실험실표준화협회(CLSI)규정적표준진행；병원균분포화약민시험결과응용 Whonet5.6연건진행통계분석。결과종림상송검적63229빈혈액표본중，배양양성4914빈，양성솔7.77%，공검출병원균2196주，혁란음성균주요위대장애희균、폐염극뢰백균、포만불동간균、동록가단포균，검출솔분별위25.50%、11.47%、7.14%、5.91%。혁란양성균중이응고매음성포도구균、금황색포도구균、장구균、초록색련구위주，분리솔분별위10.40%、7.42%、6.87%、3.91%。진균분리솔위7.40%(164/2196)，산초엄보β-내선알매적대장애희균화폐염극뢰백균분리솔분별위75.53%、55.51%。결론혈류감염병원균주요위대장애희균、폐염극뢰백균、응고매음성포도구균、금황색포도구균，림상응엄격안혈배양표본적조작규범집행，피면오염병제고송검질량。
Objective To dynamic monitor and analyze pathogen distribution and drug resistance changes isolated from blood culture samples in our hospital from 2007 to 2013 years.Methods BACTEC automated blood culture system was used to detect 63 229 blood specimens,automatic identification of bacterial identification analyzer was applied to identify species,bacterial suscepti-bility testing was detected by K-B method according to the American Society for Clinical Laboratory Standards Institute (CLSI)re-quirements standards.Pathogen distribution and antimicrobial susceptibility test results applied Whonet5.6 software for statistical analysis.Results In 63 229 blood specimens,the positive rate was 7.77% (4 914 specimens),2 1 96 strains were detected,mainly Gram-negative bacteria were Escherichia coli,Klebsiella pneumoniae,Acinetobacter baumannii,Pseudomonas aeruginosa,the detec-tion rates were 25.50%,1 1.47%,7.14%,5.91%.Gram-positive bacteria to coagulase-negative staphylococci,Staphylococcus au-reus,Enterococcus,grass green hammer main separation rates were 10.40%,7.42%,6.87%,3.91%.Fungal 7.40%(1 64/2 1 96), producing ESBLs of Escherichia coli and Klebsiella pneumoniae separation rates were 75.53%,55.5 1%.Conclusion Bloodstream infection pathogen distribution as Escherichia coli,Klebsiella pneumoniae,coagulase-negative staphylococci,Staphylococcus aureus. Clinical blood culture specimens should be strictly in accordance with the norms adopted send execution to avoid pollution and im-prove the quality inspection.
