CAJ | 학술논문

背景：食管癌是常见的消化道恶性肿瘤，预后较差，目前缺乏对其有效的化疗药物。研究发现强心苷类药物可抑制肿瘤细胞生长，但其作用机制尚未完全明确。目的：探讨哇巴因对人食管癌细胞的增殖调控作用及其机制。方法：以哇巴因处理人食管癌细胞 OE19，以 DMSO 处理的 OE19细胞作为对照组。采用细胞计数法检测细胞增殖情况；采用 real-time PCR 检测 Sox2、Sox4、Sox7、Sox9、Sox10 mRNA 表达；采用蛋白质印迹法检测 Sox4蛋白表达；采用免疫荧光染色检测细胞增殖标记物磷酸化组蛋白 H3（ph3）和 Sox4基因表达。结果：哇巴因（≥40 nmol/ L）可显著抑制 OE19细胞增殖。哇巴因40 nmol/ L 组 OE19细胞 Sox4 mRNA 和蛋白相对表达量较对照组显著降低（ P <0.05），Sox2、Sox7、Sox9、Sox10 mRNA 表达与对照组相比则无明显差异（P >0.05）；细胞核内 ph3和 Sox4基因表达较对照组显著减弱。结论：哇巴因可能通过下调 Sox4表达，调节细胞周期，从而抑制人食管癌细胞增殖。
배경：식관암시상견적소화도악성종류，예후교차，목전결핍대기유효적화료약물。연구발현강심감류약물가억제종류세포생장，단기작용궤제상미완전명학。목적：탐토왜파인대인식관암세포적증식조공작용급기궤제。방법：이왜파인처리인식관암세포 OE19，이 DMSO 처리적 OE19세포작위대조조。채용세포계수법검측세포증식정황；채용 real-time PCR 검측 Sox2、Sox4、Sox7、Sox9、Sox10 mRNA 표체；채용단백질인적법검측 Sox4단백표체；채용면역형광염색검측세포증식표기물린산화조단백 H3（ph3）화 Sox4기인표체。결과：왜파인（≥40 nmol/ L）가현저억제 OE19세포증식。왜파인40 nmol/ L 조 OE19세포 Sox4 mRNA 화단백상대표체량교대조조현저강저（ P <0.05），Sox2、Sox7、Sox9、Sox10 mRNA 표체여대조조상비칙무명현차이（P >0.05）；세포핵내 ph3화 Sox4기인표체교대조조현저감약。결론：왜파인가능통과하조 Sox4표체，조절세포주기，종이억제인식관암세포증식。
Background:Esophageal cancer is a common gastrointestinal cancer with poor prognosis,and effective chemotherapy is lacking currently. Studies have shown that cardiac glycosides can inhibit tumor cells growth,but its mechanism has not been fully clarified. Aims:To investigate the effect and mechanism of ouabain in regulating proliferation of human esophageal carcinoma cells. Methods:OE19 human esophageal carcinoma cells were treated with ouabain,and cells in control group were treated with DMSO. Cell proliferation was assessed by cell counting method. mRNA expressions of Sox2,Sox4,Sox7,Sox9 and Sox10 were determined by real-time PCR. Protein expression of Sox4 was determined by Western blotting. Gene expressions of phospho-histone3( ph3),a cell proliferation marker and Sox4 were detected by immunofluorescence staining. Results:Ouabain( ≥ 40 nmol/ L)could significantly inhibit OE19 cells proliferation. mRNA and protein expressions of Sox4 were significantly decreased in OE19 cells in ouabain(40 nmol/ L)group than those in control group(P < 0. 05). No significant differences in mRNA expressions of Sox2,Sox7,Sox9 and Sox10 were found between the two groups(P > 0. 05). Gene expressions of ph3 and Sox4 in nucleus of OE19 cells were decreased in ouabain (40 nmol/ L)group than those in control group. Conclusions:Ouabain is effective in inhibiting human esophageal carcinoma cells proliferation,the underlying mechanism might be related with down-regulation of Sox4 expression and the subsequent cell cycle modulation.