海峡药学
海峽藥學
해협약학
Strait Pharmaceutical Journal
2015年
9期
59-60
,共2页
八珍丸(浓缩丸)%芍药苷%高效液相色谱
八珍汍(濃縮汍)%芍藥苷%高效液相色譜
팔진환(농축환)%작약감%고효액상색보
BaZhen Pills ( Concentrated pills )%Paeoniflorin%HPLC
目的:建立HPLC法测定八珍丸(浓缩丸)中芍药苷的含量。方法采用 Agilent C18(4.6mm ×100mm,2.7μm)色谱柱,以乙腈∶0.1%磷酸(17∶83)为流动相,流速为0.4mL· min -1,检测波长为230nm。结果芍药苷的线性范围为20.3~406ng(r =0.9998),平均回收率为99.54%,RSD为0.8%( n=9)。结论该方法结果准确,灵敏度高,重复性好,可用于八珍丸(浓缩丸)的质量控制。
目的:建立HPLC法測定八珍汍(濃縮汍)中芍藥苷的含量。方法採用 Agilent C18(4.6mm ×100mm,2.7μm)色譜柱,以乙腈∶0.1%燐痠(17∶83)為流動相,流速為0.4mL· min -1,檢測波長為230nm。結果芍藥苷的線性範圍為20.3~406ng(r =0.9998),平均迴收率為99.54%,RSD為0.8%( n=9)。結論該方法結果準確,靈敏度高,重複性好,可用于八珍汍(濃縮汍)的質量控製。
목적:건립HPLC법측정팔진환(농축환)중작약감적함량。방법채용 Agilent C18(4.6mm ×100mm,2.7μm)색보주,이을정∶0.1%린산(17∶83)위류동상,류속위0.4mL· min -1,검측파장위230nm。결과작약감적선성범위위20.3~406ng(r =0.9998),평균회수솔위99.54%,RSD위0.8%( n=9)。결론해방법결과준학,령민도고,중복성호,가용우팔진환(농축환)적질량공제。
ABSTRACT:OBJECTIVE To establish a method for the determination of paeoniflorin in BaZhen Pills ( Concen-trated pills) by HPLC.METHODS A Agilent C18(4.6mm ×100mm,2.7μm) column was used,the mobile phase consisted of acetonitrile and 0.1%phosphoric acid (17∶83) at the flow rate of 0.4mL· min-1.The detection wave-length was 230 nm.RESULTS A good linear correlation of paeoniflorin was obtained within the range of 20.3~406ng(r=0.9998),and the average recovery was 99.54% with RSD of 0.8%(n =9).CONCLUSION The method is accurate ,sensitive and reproducible ,and can be used in the quality control of BaZhen Pills ( Concentrated pills).