胃肠病学
胃腸病學
위장병학
Chinese Journal of Gastroenterology
2015年
9期
517-522
,共6页
Faecalibacterium prausnitzii%氧化偶氮甲烷%硫酸葡聚糖%结肠肿瘤%细胞因子类
Faecalibacterium prausnitzii%氧化偶氮甲烷%硫痠葡聚糖%結腸腫瘤%細胞因子類
Faecalibacterium prausnitzii%양화우담갑완%류산포취당%결장종류%세포인자류
Faecalibacterium prausnitzii%Azoxymethane%Dextran Sulfate%Colonic Neoplasms%Cytokines
背景：Faecalibacterium prausnitzii（Fp）是人类肠道中数量最占优势的细菌之一，与结肠炎相关结直肠癌（CAC）的发生密切相关。目的：研究 Fp 对 CAC 发生的影响，并初步探讨可能的机制。方法：应用氧化偶氮甲烷（AOM）和葡聚糖硫酸钠（DSS）诱导 CAC 模型，将52只 C57BL/6J 小鼠随机分为 A 组（AOM + DSS）、B 组（AOM + DSS + Fp）、C 组（AOM + DSS + Fp 上清）、D 组（空白对照），第92天处死小鼠。评估 DAI，ELISA 法检测血清 TNF-α和 IL-10含量；HE 染色观察肿瘤分级；免疫组化法检测肠道肿瘤组织中 VEGF、COX-2、NF-κB 表达。结果：A、B、C 组小鼠成瘤率分别为100%、100%和77.8%，主要为高级别上皮内瘤变。A 组肿瘤负荷显著高于 B 组（P <0.01），B 组脾脏指数显著高于 C 组（P <0.01）。A 组血清 TNF-α含量显著低于 B 组（P <0.05），IL-10显著升高（P <0.05）。A、B、C组肠道肿瘤组织中 VEGF、COX-2、NF-κB 表达无明显差异。结论：Fp 活菌对实验性小鼠肠道肿瘤的发生率无明显影响，而其上清能减少肿瘤发生率。Fp 活菌及其上清均可能通过调节 TNF-α、IL-10表达来减轻肠道肿瘤负荷。
揹景：Faecalibacterium prausnitzii（Fp）是人類腸道中數量最佔優勢的細菌之一，與結腸炎相關結直腸癌（CAC）的髮生密切相關。目的：研究 Fp 對 CAC 髮生的影響，併初步探討可能的機製。方法：應用氧化偶氮甲烷（AOM）和葡聚糖硫痠鈉（DSS）誘導 CAC 模型，將52隻 C57BL/6J 小鼠隨機分為 A 組（AOM + DSS）、B 組（AOM + DSS + Fp）、C 組（AOM + DSS + Fp 上清）、D 組（空白對照），第92天處死小鼠。評估 DAI，ELISA 法檢測血清 TNF-α和 IL-10含量；HE 染色觀察腫瘤分級；免疫組化法檢測腸道腫瘤組織中 VEGF、COX-2、NF-κB 錶達。結果：A、B、C 組小鼠成瘤率分彆為100%、100%和77.8%，主要為高級彆上皮內瘤變。A 組腫瘤負荷顯著高于 B 組（P <0.01），B 組脾髒指數顯著高于 C 組（P <0.01）。A 組血清 TNF-α含量顯著低于 B 組（P <0.05），IL-10顯著升高（P <0.05）。A、B、C組腸道腫瘤組織中 VEGF、COX-2、NF-κB 錶達無明顯差異。結論：Fp 活菌對實驗性小鼠腸道腫瘤的髮生率無明顯影響，而其上清能減少腫瘤髮生率。Fp 活菌及其上清均可能通過調節 TNF-α、IL-10錶達來減輕腸道腫瘤負荷。
배경：Faecalibacterium prausnitzii（Fp）시인류장도중수량최점우세적세균지일，여결장염상관결직장암（CAC）적발생밀절상관。목적：연구 Fp 대 CAC 발생적영향，병초보탐토가능적궤제。방법：응용양화우담갑완（AOM）화포취당류산납（DSS）유도 CAC 모형，장52지 C57BL/6J 소서수궤분위 A 조（AOM + DSS）、B 조（AOM + DSS + Fp）、C 조（AOM + DSS + Fp 상청）、D 조（공백대조），제92천처사소서。평고 DAI，ELISA 법검측혈청 TNF-α화 IL-10함량；HE 염색관찰종류분급；면역조화법검측장도종류조직중 VEGF、COX-2、NF-κB 표체。결과：A、B、C 조소서성류솔분별위100%、100%화77.8%，주요위고급별상피내류변。A 조종류부하현저고우 B 조（P <0.01），B 조비장지수현저고우 C 조（P <0.01）。A 조혈청 TNF-α함량현저저우 B 조（P <0.05），IL-10현저승고（P <0.05）。A、B、C조장도종류조직중 VEGF、COX-2、NF-κB 표체무명현차이。결론：Fp 활균대실험성소서장도종류적발생솔무명현영향，이기상청능감소종류발생솔。Fp 활균급기상청균가능통과조절 TNF-α、IL-10표체래감경장도종류부하。
Background:Faecalibacterium prausnitzii( Fp) is one of the most abundant bacterium in human intestinal microbiota,and is closely correlated with the process of colitis-associated colorectal cancer(CAC). Aims:To observe the effect of Fp on CAC,and investigate the possible mechanism. Methods:The model of CAC was induced by azoxymethane (AOM)and dextran sodium sulfate( DSS). Fifty-two C57BL/ 6J mice were randomly divided into 4 groups:group A (AOM + DSS),group B(AOM + DSS + Fp),group C(AOM + DSS + Fp supernatant)and group D(control group). All the mice were sacrificed on day 92. DAI was assessed,serum levels of TNF-α and IL-10 were determined by ELISA. HE staining was used to examine the grade of tumor. Expressions of VEGF,COX-2,NF-κB in tumor tissue were measured by immunohistochemistry. Results:The tumorigenesis rates of group A,B,C were 100% ,100% and 77. 8% ,respectively;mainly were high-grade intraepithelial neoplasia. The tumor load in group A was significantly higher than that in group B (P < 0. 01),and the spleen index in group B was significantly higher than that in group C(P < 0. 01). Serum level of TNF-α was significantly lower(P < 0. 05)and IL-10 was significantly higher(P < 0. 05)in group A than that in group B. No significant differences in expressions of VEGF,COX-2,NF-κB were found among group A,B and C. Conclusions:Fp had no obvious effect on the occurrence rate of CAC,and Fp supernatant could decrease the incidence of CAC in mice. Fp and its supernatant could reduce the tumor load via regulating the expressions of TNF-α,IL-10.
