国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
International Journal of Laboratory Medicine
2015年
19期
2779-2781
,共3页
过敏原%特异性 IgE%荧光酶联免疫吸附法
過敏原%特異性 IgE%熒光酶聯免疫吸附法
과민원%특이성 IgE%형광매련면역흡부법
allergen%allergen specific immunoglobulin E%fluorescence enzyme linked immunosorbent assay
目的：探讨过敏性疾病患者常见过敏原特异性 IgE阳性分布特征。方法选择该院2014年3~10月疑似过敏性疾病患者1884例，采用荧光酶联免疫吸附法有针对性地对其血清进行4种吸入性过敏原组合(尘螨类、霉菌类、动物表皮类、花粉类)和4种食入性过敏原组合(海鲜类、谷类、肉类、奶制品)的特异性 IgE 检测，分析各类过敏原组合的特异性 IgE 表达情况，并观察过敏患者特异性 IgE 的性别与年龄分布差异。结果吸入性过敏原中尘螨类占73.9%、动物表皮类8.5%、霉菌类6.5%、花粉类4.0%；食入性过敏原中海鲜类占47.8%、奶制品31.5%、谷类23.0%、肉类8.5%。同时有2种或2种以上混合过敏原阳性的过敏性疾病患者高达82.0%，且尘螨类和动物表皮类常混合致敏。不同性别过敏原阳性率差异无统计学意义(P >0.05)。过敏原特异性 IgE 阳性率随着年龄增加而降低。结论尘螨类是主要的吸入性过敏原组合；海鲜类、奶制品和谷类是主要的食入性过敏原组合，过敏患者多见混合过敏原致敏，随着年龄增长过敏原阳性率逐渐降低。
目的：探討過敏性疾病患者常見過敏原特異性 IgE暘性分佈特徵。方法選擇該院2014年3~10月疑似過敏性疾病患者1884例，採用熒光酶聯免疫吸附法有針對性地對其血清進行4種吸入性過敏原組閤(塵螨類、黴菌類、動物錶皮類、花粉類)和4種食入性過敏原組閤(海鮮類、穀類、肉類、奶製品)的特異性 IgE 檢測，分析各類過敏原組閤的特異性 IgE 錶達情況，併觀察過敏患者特異性 IgE 的性彆與年齡分佈差異。結果吸入性過敏原中塵螨類佔73.9%、動物錶皮類8.5%、黴菌類6.5%、花粉類4.0%；食入性過敏原中海鮮類佔47.8%、奶製品31.5%、穀類23.0%、肉類8.5%。同時有2種或2種以上混閤過敏原暘性的過敏性疾病患者高達82.0%，且塵螨類和動物錶皮類常混閤緻敏。不同性彆過敏原暘性率差異無統計學意義(P >0.05)。過敏原特異性 IgE 暘性率隨著年齡增加而降低。結論塵螨類是主要的吸入性過敏原組閤；海鮮類、奶製品和穀類是主要的食入性過敏原組閤，過敏患者多見混閤過敏原緻敏，隨著年齡增長過敏原暘性率逐漸降低。
목적：탐토과민성질병환자상견과민원특이성 IgE양성분포특정。방법선택해원2014년3~10월의사과민성질병환자1884례，채용형광매련면역흡부법유침대성지대기혈청진행4충흡입성과민원조합(진만류、매균류、동물표피류、화분류)화4충식입성과민원조합(해선류、곡류、육류、내제품)적특이성 IgE 검측，분석각류과민원조합적특이성 IgE 표체정황，병관찰과민환자특이성 IgE 적성별여년령분포차이。결과흡입성과민원중진만류점73.9%、동물표피류8.5%、매균류6.5%、화분류4.0%；식입성과민원중해선류점47.8%、내제품31.5%、곡류23.0%、육류8.5%。동시유2충혹2충이상혼합과민원양성적과민성질병환자고체82.0%，차진만류화동물표피류상혼합치민。불동성별과민원양성솔차이무통계학의의(P >0.05)。과민원특이성 IgE 양성솔수착년령증가이강저。결론진만류시주요적흡입성과민원조합；해선류、내제품화곡류시주요적식입성과민원조합，과민환자다견혼합과민원치민，수착년령증장과민원양성솔축점강저。
Objective To explore the positive distribution characteristics of common allergen specific immunoglobulin E(slgE)in patients with suspected allergy in the Affiliated Zhongshan Hospital of Sun Yat-sen University.Methods Fluorescence enzyme linked immunosorbent assay was used to detect the serum sIgE antibody of 1 884 patients with suspected allergy diseases,including four kinds of inhalant allergens combinations (dust mites,mold,animal skin,pollen)and four kinds of food allergens combinations (seafood,cereals,meat,dairy products)from March to October 2014.The slgE expression situation of different allergen,different gender and age in allergic patients were compared.Results The slgE positive rates of dust mites,animal fur,mildew,pollen in in-haled allergens were 73.9%,8.5%,6.5%,4.0%.The positive rates of seafood,dairy products,cereals,meat in food allergens were 47.8%,31.5%,23.0%,8.5%.The slgE positive rate of patients with allergic diseases who were allergic to two kinds or more mixed allergens was up to 82.0%,and often allergic to dust mites and animal fur.The slgE positive rate of different gender had no significant difference (P >0.05).The slgE positive rate decreased as age increased.Conclusion Dust mites are the main inhalant al-lergens combination,seafood,dairy products and cereals are the main food allergens combinations.Patients with allergic disease are allergic mixed allergens.SlgE positive rate decreases with increasing age.
