中国卫生标准管理
中國衛生標準管理
중국위생표준관리
China Health Standard Management
2015年
26期
154-156
,共3页
脑缺血%骨髓间充质干细胞%Notch1
腦缺血%骨髓間充質榦細胞%Notch1
뇌결혈%골수간충질간세포%Notch1
Cerebral ischemia%BMSCs%Notch1
目的：探讨骨髓间充质干细胞移植对大鼠脑缺血后Notch1-Dl4信号通路的影响。方法60只SD大鼠随机分为假手术组，MCAO组， BMSC组，DAPT组。制备大鼠MCAO模型，BMSC组及DAPT组分别于造模成功后24 h经侧脑室注入BMSCs或含DAPT的BMSCs，术后4 d、7 d、14 d取脑，计数脑缺血周边区微血管数量，RT-PCR法检测Notch1、Dl4的表达。结果 BMSC组大鼠脑缺血周边区的微血管数量明显多于MCAO组及DAPT组（P＜0.05）；BMSC组大鼠Notch1、Dl 4表达水平较MCAO组及DAPT组明显增高（P＜0.05）。结论骨髓间充质干细胞移植可能通过上调Notch1-Dl 4信号通路的表达来促进大鼠脑缺血周边区血管新生。
目的：探討骨髓間充質榦細胞移植對大鼠腦缺血後Notch1-Dl4信號通路的影響。方法60隻SD大鼠隨機分為假手術組，MCAO組， BMSC組，DAPT組。製備大鼠MCAO模型，BMSC組及DAPT組分彆于造模成功後24 h經側腦室註入BMSCs或含DAPT的BMSCs，術後4 d、7 d、14 d取腦，計數腦缺血週邊區微血管數量，RT-PCR法檢測Notch1、Dl4的錶達。結果 BMSC組大鼠腦缺血週邊區的微血管數量明顯多于MCAO組及DAPT組（P＜0.05）；BMSC組大鼠Notch1、Dl 4錶達水平較MCAO組及DAPT組明顯增高（P＜0.05）。結論骨髓間充質榦細胞移植可能通過上調Notch1-Dl 4信號通路的錶達來促進大鼠腦缺血週邊區血管新生。
목적：탐토골수간충질간세포이식대대서뇌결혈후Notch1-Dl4신호통로적영향。방법60지SD대서수궤분위가수술조，MCAO조， BMSC조，DAPT조。제비대서MCAO모형，BMSC조급DAPT조분별우조모성공후24 h경측뇌실주입BMSCs혹함DAPT적BMSCs，술후4 d、7 d、14 d취뇌，계수뇌결혈주변구미혈관수량，RT-PCR법검측Notch1、Dl4적표체。결과 BMSC조대서뇌결혈주변구적미혈관수량명현다우MCAO조급DAPT조（P＜0.05）；BMSC조대서Notch1、Dl 4표체수평교MCAO조급DAPT조명현증고（P＜0.05）。결론골수간충질간세포이식가능통과상조Notch1-Dl 4신호통로적표체래촉진대서뇌결혈주변구혈관신생。
Objective To investigate the effects of BMSCs on the expression of Notch1-Dl4 in the brains of rats with focal cerebral ischemia. MethodSixty male adult Sprague-Dawley rats were randomly divided into 4 groups: Sham group, MCAO group, BMSC group, DAPT group. 24 hours after MCAO, BMSCs were injected into the lateral ventricle of rats in BMSC group, and the same dose of BMSCs contained DAPT was given to the rats in DAPT group. Rats are sacrificed at 4 d, 7 d and 14 d after MCAO. The amount of were counted, and the level of Notch1, Dl4 were detected by RT-PCR. ResultsThe amount of microvessels increased in BMSC group (P<0.05). Compared with the MCAO group, the Notch1, Dl4 expression in ischemic brain was upregulated (P<0.05).Conclusion BMSCs can improve the by upregulating the expression of Notch1-Dl4 signaling pathway after MCAO in rats.
