海峡药学
海峽藥學
해협약학
Strait Pharmaceutical Journal
2015年
9期
45-46
,共2页
虫草菌丝益肾胶囊%马钱苷%高效液相色谱法
蟲草菌絲益腎膠囊%馬錢苷%高效液相色譜法
충초균사익신효낭%마전감%고효액상색보법
Chongcao Junsi Yishen capsules%Loganin%HPLC
目的:建立HPLC法测定虫草菌丝益肾胶囊中马钱苷含量的方法。方法色谱柱为 Hypersil C18(250mm ×4.6mm,5μm),流动相为乙腈-水(15∶85),流速为1.0mL· min -1,检测波长为240nm。结果此法线性范围为0.0391~1.956μg(r=0.9999),平均回收率为102.50%, RSD=1.80%(n=6)。结论该方法简便、快速,重现性好,可用于虫草菌丝益肾胶囊的质量控制。
目的:建立HPLC法測定蟲草菌絲益腎膠囊中馬錢苷含量的方法。方法色譜柱為 Hypersil C18(250mm ×4.6mm,5μm),流動相為乙腈-水(15∶85),流速為1.0mL· min -1,檢測波長為240nm。結果此法線性範圍為0.0391~1.956μg(r=0.9999),平均迴收率為102.50%, RSD=1.80%(n=6)。結論該方法簡便、快速,重現性好,可用于蟲草菌絲益腎膠囊的質量控製。
목적:건립HPLC법측정충초균사익신효낭중마전감함량적방법。방법색보주위 Hypersil C18(250mm ×4.6mm,5μm),류동상위을정-수(15∶85),류속위1.0mL· min -1,검측파장위240nm。결과차법선성범위위0.0391~1.956μg(r=0.9999),평균회수솔위102.50%, RSD=1.80%(n=6)。결론해방법간편、쾌속,중현성호,가용우충초균사익신효낭적질량공제。
ABSTRACT:OBJECTIVE To establish a HPLC method for the determination of Loganin in Chongcao Junsi Yish-en capsules.METHODS The Hypersil C18 colum (250mm ×4.6mm,5μm) was used.The mobile phase consisted of acetonitrile-water(15∶85) with flow rate at 1.0mL· min -1.The detection wavelenghth was at 240nm.RESULTS The method was linear in the range of 0.0391~1.956μg( r=0.9999 ).The average recovery was 102.50% and RSD was 1.80%( n=6 ).CONCLUSION The method is efficient and accurate with a good repeatability and can be used for the control of Chongcao Junsi Yishen capsules.