CAJ | 학술논문

目的：建立GC-MS测定香砂平胃丸中乙酸龙脑酯含量的方法。方法采用 DB-5MS UI 毛细管柱，高纯氦气为载气，流速：1.0mL · min －1；进样口温度：250℃，程序升温模式，起始70℃，保持2min，以20℃／min的速率升温至210℃，保持1min；质谱检测器检测，溶剂延迟3min，选择离子监测（ SIM）模式，监测定量离子m／z 121、136；样品粉碎后采用无水乙醇超声提取。结果乙酸龙脑酯在0.205～8.216μg· mL －1（ r＝0.0.9998）范围内线性关系良好，平均回收率为103.71％、RSD为2.57％（n＝6），最低检出限为0.0082μg· mL －1。结论该方法简便可靠，灵敏度高，可控制香砂平胃丸的质量。
목적：건립GC-MS측정향사평위환중을산용뇌지함량적방법。방법채용 DB-5MS UI 모세관주，고순양기위재기，류속：1.0mL · min －1；진양구온도：250℃，정서승온모식，기시70℃，보지2min，이20℃／min적속솔승온지210℃，보지1min；질보검측기검측，용제연지3min，선택리자감측（ SIM）모식，감측정량리자m／z 121、136；양품분쇄후채용무수을순초성제취。결과을산용뇌지재0.205～8.216μg· mL －1（ r＝0.0.9998）범위내선성관계량호，평균회수솔위103.71％、RSD위2.57％（n＝6），최저검출한위0.0082μg· mL －1。결론해방법간편가고，령민도고，가공제향사평위환적질량。
ABSTRACT:OBJECTIVE To establish a method for the determination of bornyl acetate in Xiangshapingwei Pills by GC-MS.METHODS DB-5MS UI column was used with the carries gas of Helium and the flow rate of carries gas was 1.0 mL· min -1;the inlet temperature was 250℃;the column temperature rose by program:the initial temper-ature was 70℃and maintained for 2min,and rose at 20℃/min up to 210℃ for 1min.The MSD detector was used and the time of solvent delay was 3 min;selected ion monitorine mode was used with the target quantitative ion of m /z 121 ,136.Crushed samples were extracted by anhydrous ethanol.RESULTS The linearities of bornyl acetate was fairly good and linear ranges was 0.205~8.216μg · mL-1 ( r=0.0.9998 );the average recoveries was 103.71%with RSD of 2.57%(n=6);the limit of detection was 0.0082μg· mL-1.CONCLUSION The method is simple, reliable and high sensitive ,it can be used for quality control of Xiangshapingwei Pills.