中国当代医药
中國噹代醫藥
중국당대의약
China Modern Medicine
2015年
27期
14-16
,共3页
RNA干预%骨髓间充质干细胞%骨质疏松%骨密度%I型前胶原氨基端前肽%β-I型胶原羧基端肽
RNA榦預%骨髓間充質榦細胞%骨質疏鬆%骨密度%I型前膠原氨基耑前肽%β-I型膠原羧基耑肽
RNA간예%골수간충질간세포%골질소송%골밀도%I형전효원안기단전태%β-I형효원최기단태
RNA interference%Bone marrow mesenchymal stem cells%Osteoporosis%Bone mineral density%Procollagen typeⅠ N-terminal propeptide%β-C-terminal cross-linking telopeptide of typeⅠcollagen
目的：探讨抑制骨髓间充质干细胞（BMSCs）成脂分化能力对骨质疏松（OP）的早期疗效。方法选取雌性SD大鼠40只，采用背侧入路摘除卵巢法建立大鼠OP模型。随机分为4组：A组（假手术组）、B组（模型组）、C组（BMSCs治疗组）、D组（RNAi转染BMSCs治疗组），每组10只。造模后7 d经尾静脉分别注射生理盐水、10%胎牛血清的LG-DMEM培养液、BMSCs、RNAi转染BMSCs，BMSCs来自课题组从SD大鼠骨髓中提取并经过流式细胞仪和诱导分化染色鉴定的BMSCs，RNAi转染BMSCs来自课题组前一部分体外实验中使用腺病毒转染后的BMSCs。干预后，依照分组行骨密度（BMD）、骨转换标志物检测。比较4组大鼠的骨转换标志物在OP早期骨形成与骨吸收过程中的变化。结果D组的BMD显著高于B、C组（P<0.05），而与A组差异无统计学意义（P>0.05）；D组的PINP高于B、C组（P<0.05），而与A组差异无统计学意义（P>0.05）；D组的β-CTX低于B、C组（P<0.05），而与A组差异无统计学意义（P>0.05）。结论 RNAi转染BMSCs可提高去势大鼠的BMD，促进骨形成。
目的：探討抑製骨髓間充質榦細胞（BMSCs）成脂分化能力對骨質疏鬆（OP）的早期療效。方法選取雌性SD大鼠40隻，採用揹側入路摘除卵巢法建立大鼠OP模型。隨機分為4組：A組（假手術組）、B組（模型組）、C組（BMSCs治療組）、D組（RNAi轉染BMSCs治療組），每組10隻。造模後7 d經尾靜脈分彆註射生理鹽水、10%胎牛血清的LG-DMEM培養液、BMSCs、RNAi轉染BMSCs，BMSCs來自課題組從SD大鼠骨髓中提取併經過流式細胞儀和誘導分化染色鑒定的BMSCs，RNAi轉染BMSCs來自課題組前一部分體外實驗中使用腺病毒轉染後的BMSCs。榦預後，依照分組行骨密度（BMD）、骨轉換標誌物檢測。比較4組大鼠的骨轉換標誌物在OP早期骨形成與骨吸收過程中的變化。結果D組的BMD顯著高于B、C組（P<0.05），而與A組差異無統計學意義（P>0.05）；D組的PINP高于B、C組（P<0.05），而與A組差異無統計學意義（P>0.05）；D組的β-CTX低于B、C組（P<0.05），而與A組差異無統計學意義（P>0.05）。結論 RNAi轉染BMSCs可提高去勢大鼠的BMD，促進骨形成。
목적：탐토억제골수간충질간세포（BMSCs）성지분화능력대골질소송（OP）적조기료효。방법선취자성SD대서40지，채용배측입로적제란소법건립대서OP모형。수궤분위4조：A조（가수술조）、B조（모형조）、C조（BMSCs치료조）、D조（RNAi전염BMSCs치료조），매조10지。조모후7 d경미정맥분별주사생리염수、10%태우혈청적LG-DMEM배양액、BMSCs、RNAi전염BMSCs，BMSCs래자과제조종SD대서골수중제취병경과류식세포의화유도분화염색감정적BMSCs，RNAi전염BMSCs래자과제조전일부분체외실험중사용선병독전염후적BMSCs。간예후，의조분조행골밀도（BMD）、골전환표지물검측。비교4조대서적골전환표지물재OP조기골형성여골흡수과정중적변화。결과D조적BMD현저고우B、C조（P<0.05），이여A조차이무통계학의의（P>0.05）；D조적PINP고우B、C조（P<0.05），이여A조차이무통계학의의（P>0.05）；D조적β-CTX저우B、C조（P<0.05），이여A조차이무통계학의의（P>0.05）。결론 RNAi전염BMSCs가제고거세대서적BMD，촉진골형성。
Objective To explore the early curative effect of restraining fat differentiation ability of bone marrow mes-enchymal stem cells (BMSCs) in osteoporosis (OP). Methods 40 female SD rats were selected.Osteoporotic of rats model was established by dorsal approach ovary removal method.Rats were randomly divided into four groups:group A (sham-operated group),group B (model group),group C (BMSCs treatment group) and group D (RNAi transfection BMSCs treat-ment group).Each group has 10 rats.After 7 days moldeling,rats in four groups were injected saline,LG-DMEM culture contained 10% fetal bovine serum,BMSCs and RNAi transfection BMSCs.BMSCs were extracted from SD rat bone mar-row of and identified by flow cytometer and induced differentiation dyeing.RNAi transfection BMSCs were from the transfected BMSCs of adenovirus used for the former parts of in v itro experiment in research group.After intervention, bone mineral density (BMD) and markers of bone turnover were tested according to grouping.The change of bone turnover markers of rats in bone formation and bone resorption of the early osteoporosis was compared among four groups. Re-sults BMD in group D was higher than that of group B and C (P<0.05),but had no significant difference compared with group A (P>0.05).PINP in group D was higher than that of group B and C (P<0.05),but had no significant difference compared with group A (P>0.05).β-CTX in D group was lower than that of group B and C (P<0.05),but had no significant differ-ence compared with group A (P>0.05). Conclusion RNAi transfection BMSCs can improve BMD of castration rats and pro-mote bone formation.
