CAJ | 학술논문

通过过表达酿酒酵母异丁醇合成途径中的相关基因，以及缺失编码合成甘油和乙醇的关键基因，来提高异丁醇产率。过表达酿酒酵母异丁醇合成途径中，乙酰乳酸合酶（Ilv2）和支链氨基酸转氨酶（Bat2）并缺失编码甘油合成的关键基因GPD2得到菌株 HZAL-13（YEplac181-PGK1p-ILV2ORF ），其厌氧发酵异丁醇产率比出发菌株增加3．91倍；在 HZAL-13的基础上进一步缺失乙醇合成途径中编码丙酮酸脱羧酶的关键基因 PDC6，得到菌株 HZAL-14（YEplac181-PGK1p-ILV2ORF ），其厌氧发酵异丁醇产率较出发菌株增加8．97倍。研究结果表明减少副产物甘油和乙醇的生物合成有助于提高异丁醇的产率，利用基因工程方法缺失GPD2基因为提高异丁醇产率提供了新方向。
통과과표체양주효모이정순합성도경중적상관기인，이급결실편마합성감유화을순적관건기인，래제고이정순산솔。과표체양주효모이정순합성도경중，을선유산합매（Ilv2）화지련안기산전안매（Bat2）병결실편마감유합성적관건기인GPD2득도균주 HZAL-13（YEplac181-PGK1p-ILV2ORF ），기염양발효이정순산솔비출발균주증가3．91배；재 HZAL-13적기출상진일보결실을순합성도경중편마병동산탈최매적관건기인 PDC6，득도균주 HZAL-14（YEplac181-PGK1p-ILV2ORF ），기염양발효이정순산솔교출발균주증가8．97배。연구결과표명감소부산물감유화을순적생물합성유조우제고이정순적산솔，이용기인공정방법결실GPD2기인위제고이정순산솔제공료신방향。
Isobutanol is regarded as a next-generation biofuel for higher octane number and higher energy density.Through over-expressing related genes of isobutanol biosynthesis and deleting key genes encoding glycerol and ethanol biosynthesis of Saccharo-myces cerevisiae to improve isobutanol yield.The strain HZAL-13 (YEplac181-PGK1p-ILV2ORF )was constructed by overex-pressing acetolactate synthase (Ilv2)and branched chain amino acid transaminase (Bat2)and deleting GPD2 .Another strain HZAL-14 (YEplac181-PGK1p-ILV2ORF )was obtained on the basis of HZAL-13 by further deleting the key PDC6 gene of pyruvate decarboxylase.Anaerobic fermentations show that isobutanol yield of HZAL-13 is increased 3.91 folds than that of con-trol strain;and isobutanol yield of HZAL-14 is significantly increased 8.97 folds than that of control strain.These results demon-strated that decreasing glycerol and ethanol biosynthesis can improve isobutanol yield and using gene engineering technology to de-lete GPD2 in Saccharomyces cerevisiae may provide a new way to improve isobutanol production for future.