CAJ | 학술논문

为了提高燕麦分子育种的效率，旨在开发燕麦D基因组特异分子标记。先利用300条10碱基随机引物对9种燕麦种材料进行随机多态性扩增(random amplified polymorphic DNA，RAPD)，扩增出燕麦D基因组特异性条带，命名为OPAB011287，经Blast比对后发现该条带为一反转座子重复序列，根据此条带序列设计特异性引物，建立更简便稳定的SCAR标记。结果发现该SCAR标记可以准确特异的检测到9种试验材料中含有D基因组的燕麦品种，结论表明该标记可用于燕麦分子标记辅助育种，有效服务于燕麦优良品种培育。
위료제고연맥분자육충적효솔，지재개발연맥D기인조특이분자표기。선이용300조10감기수궤인물대9충연맥충재료진행수궤다태성확증(random amplified polymorphic DNA，RAPD)，확증출연맥D기인조특이성조대，명명위OPAB011287，경Blast비대후발현해조대위일반전좌자중복서렬，근거차조대서렬설계특이성인물，건립경간편은정적SCAR표기。결과발현해SCAR표기가이준학특이적검측도9충시험재료중함유D기인조적연맥품충，결론표명해표기가용우연맥분자표기보조육충，유효복무우연맥우량품충배육。
The research aims to improve the efficiency of molecular breeding and develop D-Genome-specific markers in oat. Firstly, random amplified polymorphic DNA (RAPD) analysis was performed on 9 oat varieties with 300 random 10-based primers. A repetitive sequence of 1287 bp, named OPAB011287 was identified in the D genome of oat. BLAST search revealed that the sequence of OPAB011287 was highly homologous to a part of a retrotransposon. Based on the sequence of OPAB011287, a pair of sequence-characterized amplified region primers (SCAR) was designed which was more convenient and stable. And the results showed that SCAR marker was specific to target oat with D genome, which provided a powerful tool for oat marker assisted breeding and the better service for the breeding of oat.