CAJ | 학술논문

针对传统电子染色方法可控性差、经验性强的弱点，提出了一种无需染色的“负像法”作为表征聚丙烯酸酯类胶乳的TEM 方法。该方法首先将稀释后的胶乳溶液在铜网上进行常规的滴液制样，然后利用聚丙烯酸酯类聚合物热黏、冷脆的结构特性，对载样铜网在40℃下进行短时烘烤后直接进行电镜观测。研究结果表明，以适度烘烤样品为基础的“负像法”具有衬度高、边界清晰、核壳结构可辨的观测优势，且样品后处理简单，制样灵活，浓度依赖性低，可以方便、快捷地表征聚丙烯酸酯类胶乳的形貌和结构，并为相关体系的电镜研究提供参考和借鉴。在满足低热输入、短时烘烤和倾斜加热等条件的前提下，该方法同样适用于其他含有低玻璃化转变温度组分的核壳结构纳米微粒的电镜观测。
침대전통전자염색방법가공성차、경험성강적약점，제출료일충무수염색적“부상법”작위표정취병희산지류효유적TEM 방법。해방법수선장희석후적효유용액재동망상진행상규적적액제양，연후이용취병희산지류취합물열점、랭취적결구특성，대재양동망재40℃하진행단시홍고후직접진행전경관측。연구결과표명，이괄도홍고양품위기출적“부상법”구유츤도고、변계청석、핵각결구가변적관측우세，차양품후처리간단，제양령활，농도의뢰성저，가이방편、쾌첩지표정취병희산지류효유적형모화결구，병위상관체계적전경연구제공삼고화차감。재만족저열수입、단시홍고화경사가열등조건적전제하，해방법동양괄용우기타함유저파리화전변온도조분적핵각결구납미미립적전경관측。
A novel “negative image”method was proposed for microscopic characterization on polyacrylate latex particles with transmission electron microscope (TEM)based on the structural characteristic of “sticky when heated and fragile when cooled”for polyacrylate.After being dropped onto carbon-coated copper grids and naturally dried,the samples of latex particles were heated at 40 ℃ for certain time before direct TEM observation.This “negative image”method possesses the virtues of flexible sample preparation,simplified post treatment,low dependence on concentration,and especially no need of electron staining. TEM photographs obtained by this method have the advantages of high contrast,distinct boundaries and discernible core-shell structures.Accordingly,the “negative image”method is able to take the place of traditional electron staining and serves as a qual-ified solution to quickly and conveniently characterize polyacrylate latex.If the prerequisites of low heat input,short-time heating and tilting incident illumination are met,this method could also be applied to similar systems with components of low glass transi-tion temperatures,where the crosslinking of the outer shell of latex particles are required if necessary.