CAJ | 학술논문

为了利用蛋白质组学技术，寻找可能与 ICF45存在相互作用的蛋白，从而为探讨 ICF45蛋白的生物学功能和作用机制提供依据，本文利用免疫共沉淀和 GST-pull down 技术对 Hela 细胞的 ICF45蛋白进行富集，通过 SDS-PAGE 电泳寻找差异条带，并联用质谱进行蛋白鉴定。结果表明：2种实验方法共同筛选得到线粒体定位的分子伴侣 HSPA9及 Annexin A2与 ICF45在体内可能存在相互作用。研究成果为进一步研究 ICF45在细胞增殖和调控中的作用奠定了实验基础。
위료이용단백질조학기술，심조가능여 ICF45존재상호작용적단백，종이위탐토 ICF45단백적생물학공능화작용궤제제공의거，본문이용면역공침정화 GST-pull down 기술대 Hela 세포적 ICF45단백진행부집，통과 SDS-PAGE 전영심조차이조대，병련용질보진행단백감정。결과표명：2충실험방법공동사선득도선립체정위적분자반려 HSPA9급 Annexin A2여 ICF45재체내가능존재상호작용。연구성과위진일보연구 ICF45재세포증식화조공중적작용전정료실험기출。
To identify the potential interacting proteins with ICF45 by proteomics analysis and investigate the biological role and the mechanism of ICF45,the interaction complex of ICF45 was enriched by co-immunoprecipitation and GST-pull down in Hela cells,which was then separated by SDS-PAGE.The candidate proteins were identified by mass spectrometry.The results indi-cate mitochondrial localization chaperones HSPA9 and Annexin A2 are identified as the possible interaction protein with ICF45 by two methods,respectively.The function of ICF45 and potential interacting proteins will provide experimental basis for identifying the role of ICF45 on cell proliferation and the regulation.