医学信息
醫學信息
의학신식
Medical Information
2015年
41期
52-53
,共2页
GICA%ELISA%手足口病%抗EV-71-IGM抗体
GICA%ELISA%手足口病%抗EV-71-IGM抗體
GICA%ELISA%수족구병%항EV-71-IGM항체
GICA%ELISA%Foot and mouth disease%Anti EV-71-IGM antibody
目的：探讨胶体金免疫层析法(GICA)和酶联免疫法(ELISA)用于手足口病早期患儿抗EV-71-IGM抗体检测临床价值差异。方法选取我院近期收治手足口病早期患儿共180例，依次行胶体金免疫层析法(GICA)和酶联免疫法(ELISA)进行抗EV-71-IGM抗体检测，比较两种方法检测手足口病早期患儿抗EV-71-IGM抗体阳性率及临床诊断一致性。结果 GICA和ELISA用于手足口病早期患者抗EV-71-IGM抗体检测符合率为76.67%（138/180），临床诊断一致性检测Kappa值=0.52；GICA和ELISA用于手足口病早期患者抗EV-71-IGM抗体检测阳性率分别为47.78%（86/180），26.67%（48/180）；GICA法用于手足口病早期患者抗EV-71-IGM抗体检测阳性率显著高于ELISA法，差异有统计学意义（<0.05）。结论相较于ELISA法，GICA用于手足口病早期患儿抗EV-71-IGM抗体检测阳性率更高，且对于两种方法均检测阴性患儿，应及时复查避免漏检。
目的：探討膠體金免疫層析法(GICA)和酶聯免疫法(ELISA)用于手足口病早期患兒抗EV-71-IGM抗體檢測臨床價值差異。方法選取我院近期收治手足口病早期患兒共180例，依次行膠體金免疫層析法(GICA)和酶聯免疫法(ELISA)進行抗EV-71-IGM抗體檢測，比較兩種方法檢測手足口病早期患兒抗EV-71-IGM抗體暘性率及臨床診斷一緻性。結果 GICA和ELISA用于手足口病早期患者抗EV-71-IGM抗體檢測符閤率為76.67%（138/180），臨床診斷一緻性檢測Kappa值=0.52；GICA和ELISA用于手足口病早期患者抗EV-71-IGM抗體檢測暘性率分彆為47.78%（86/180），26.67%（48/180）；GICA法用于手足口病早期患者抗EV-71-IGM抗體檢測暘性率顯著高于ELISA法，差異有統計學意義（<0.05）。結論相較于ELISA法，GICA用于手足口病早期患兒抗EV-71-IGM抗體檢測暘性率更高，且對于兩種方法均檢測陰性患兒，應及時複查避免漏檢。
목적：탐토효체금면역층석법(GICA)화매련면역법(ELISA)용우수족구병조기환인항EV-71-IGM항체검측림상개치차이。방법선취아원근기수치수족구병조기환인공180례，의차행효체금면역층석법(GICA)화매련면역법(ELISA)진행항EV-71-IGM항체검측，비교량충방법검측수족구병조기환인항EV-71-IGM항체양성솔급림상진단일치성。결과 GICA화ELISA용우수족구병조기환자항EV-71-IGM항체검측부합솔위76.67%（138/180），림상진단일치성검측Kappa치=0.52；GICA화ELISA용우수족구병조기환자항EV-71-IGM항체검측양성솔분별위47.78%（86/180），26.67%（48/180）；GICA법용우수족구병조기환자항EV-71-IGM항체검측양성솔현저고우ELISA법，차이유통계학의의（<0.05）。결론상교우ELISA법，GICA용우수족구병조기환인항EV-71-IGM항체검측양성솔경고，차대우량충방법균검측음성환인，응급시복사피면루검。
Objective To investigate clinical value dif erences of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease including col oidal gold immunochromatography assay (GICA) and enzyme linked immunosorbent assay (ELISA). Methods 180 cases of children with early stage of foot and mouth disease were chosen in recent year in our hospital and detected anti EV-71-IGM antibody by col oidal gold immunochromatography assay (GICA) and enzyme linked immunosorbent assay (ELISA); and the positive detection rate and clinical diagnosis consistency of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease was compared. Results The detected coincidence rate of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease including GICA and ELISA was 76.67%(138/180)and Kappa value of clinical diagnosis consistency was 0.52. The positive detection rate of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease including GICA and ELISA were separately 47.78%(86/180), 26.67%(48/180);the positive detection rate of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease by GICA was significantly higher than ELISA( <0.05). Conclusion Compared with ELISA method, GICA method for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease possess higher positive detection rate; and for children with negative detection results by two kinds of methods, it should be promptly review in order to avoid missing detection.
